马齿苋的多功效研究及安全性评价
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摘要
以马齿苋作为研究对象,通过多种方法对其抑菌、美白、抗衰老、抗炎、抗敏等功效进行研究并对其安全性进行评价。结果显示,当马齿苋提取液质量浓度为125 g/L时,对5种常见菌均有抑制效果;质量浓度为2.0 g/L时,对酪氨酸酶的抑制率为82.49%,对DPPH自由基的清除率达100%,对羟自由基的清除率为92.56%,均高于同质量浓度的VC;质量浓度为150 g/L时,对小鼠耳廓肿胀抑制率为55.74%;质量浓度为1.5 g/L时,对透明质酸酶的体外抑制率为72.37%;质量浓度为200 g/L时,RBC溶血率为45.78%,半数溶血量为230 g/L;质量浓度不超过150 g/L的家兔急性眼刺激和人体安全性评价等级为安全无刺激。马齿苋具有较好的抑菌、美白、抗衰老、抗炎、抗敏功效,是一种安全无刺激的化妆品原料。
Taking purslane as the research object,the study on its functions such as bacteriostatic,whitening,anti-aging,anti-inflammation and anti-allergy,and evaluation of its safety were carried out through a variety of methods. Results show that when the mass concentration of purslane extract is 125 g/L,it can inhibit five kinds of common bacteria. When the mass concentration is 2. 0 g/L,the inhibition rate of tyrosinase is82. 49%,the scavenging rate of DPPH radical is 100%,and the scavenging rate of hydroxyl radical is92. 56%,all higher than the same mass concentration of vitamin C. When the mass concentration is 150 g/L,the mice auricle swelling inhibition rate is 55. 74%. When the mass concentration is 1. 5 g/L,the inhibition rate of hyaluronidase in vitro is 72. 37%. When the mass concentration is 200 g/L,the RBC hemolytic ratio is45. 78%,and the half hemolytic dose is 230 g/L. Results of acute eye irritation test of rabbits and human safety rating are safe and non-irritating,when the mass concentration is not exceeded 150 g/L. Purslane has good bacteriostatic,whitening,anti-aging,anti-inflammatory,and anti-allergic functions,is a safe and non-irritating cosmetics raw materials.
Taking purslane as the research object,the study on its functions such as bacteriostatic,whitening,anti-aging,anti-inflammation and anti-allergy,and evaluation of its safety were carried out through a variety of methods. Results show that when the mass concentration of purslane extract is 125 g/L,it can inhibit five kinds of common bacteria. When the mass concentration is 2. 0 g/L,the inhibition rate of tyrosinase is82. 49%,the scavenging rate of DPPH radical is 100%,and the scavenging rate of hydroxyl radical is92. 56%,all higher than the same mass concentration of vitamin C. When the mass concentration is 150 g/L,the mice auricle swelling inhibition rate is 55. 74%. When the mass concentration is 1. 5 g/L,the inhibition rate of hyaluronidase in vitro is 72. 37%. When the mass concentration is 200 g/L,the RBC hemolytic ratio is45. 78%,and the half hemolytic dose is 230 g/L. Results of acute eye irritation test of rabbits and human safety rating are safe and non-irritating,when the mass concentration is not exceeded 150 g/L. Purslane has good bacteriostatic,whitening,anti-aging,anti-inflammatory,and anti-allergic functions,is a safe and non-irritating cosmetics raw materials.
引文
[1]董银卯,邓小锋.化妆品植物原料现状、应用与发展趋势[J].轻工学报,2016,31(4):30-38.
[2]高薪,王峥,王超,等.植物提取物在化妆品中的应用[J].吉林农业,2011(4):318-319.
[3]丁怀伟,姚佳琪,宋少江.马齿苋的化学成分和药理活性研究进展[J].沈阳药科大学学报,2008(10):831-838.
[4]Iranshahy M,Javadi B,Iranshahi M,et al.A review of traditional uses,phytochemistry and pharmacology of Portulaca oleracea L[J].Journal of Ethnopharmacology,2017,205:158-172.
[5]潘姝.马齿苋中多糖和黄酮的分离纯化及其抑菌作用的研究[D].长春:吉林农业大学,2015.
[6]苏锐,张红.马齿苋黄酮抗氧化活性研究[J].安徽农业科学,2010,38(8):4068-4070.
[7]朱晓宦,吴向阳,仰榴青,等.马齿苋粗多糖的提取及清除羟自由基活性作用[J].江苏大学学报(医学版),2007,17(1):57-60.
[8]罗新星.金花茶美白功能研究及其产品制备[D].大连:大连理工大学,2015.
[9]陈文伟,肖丽英.8种中草药对耐药金葡菌的最小抑菌质量浓度检测[J].广东药学,2005,15(3):72-73.
[10]贾之慎,邬建敏,唐孟成.比色法测定Fenton反应产生的羟自由基[J].生物化学与生物物理进展,1996(2):184-186,193.
[11]董秀玥.配对t检验与成组t检验优选方法研究[J].数理医药学杂志,2010,23(1):11-14.
[12]Nandhini M,Sivanandam S N.An improved predictive association rule based classifier using gain ratio and T-test for health care data diagnosis[J].Sadhana,2015,40(9):1683-1699.
[13]区梓聪,何秋星,陈佩,等.8种中药提取物在膏霜类化妆品中的抑菌效能研究[J].日用化学工业,2017,47(3):148-152,158.
[14]Kawasaki M,Toyoda M,Teshima R,et al.In vitro antiallergic activity of flavonoids in histamine release assay using rat basophilic leukemia(RBL-2H3)cells[J].J.Food Hyg.Soc.Japan,1994,35:497-503.
[15]陆泽安,郑赛华,马来记,等.绵羊红细胞溶血试验用于化妆品眼刺激试验的动物替代方法研究[J].日用化学品科学,2013,36(1):23-27.
[16]敖华英,程树军,谈伟君.不同哺乳动物红细胞溶血替代眼刺激实验的比较[J].中国组织工程研究,2012,16(12):2163-2166.
[2]高薪,王峥,王超,等.植物提取物在化妆品中的应用[J].吉林农业,2011(4):318-319.
[3]丁怀伟,姚佳琪,宋少江.马齿苋的化学成分和药理活性研究进展[J].沈阳药科大学学报,2008(10):831-838.
[4]Iranshahy M,Javadi B,Iranshahi M,et al.A review of traditional uses,phytochemistry and pharmacology of Portulaca oleracea L[J].Journal of Ethnopharmacology,2017,205:158-172.
[5]潘姝.马齿苋中多糖和黄酮的分离纯化及其抑菌作用的研究[D].长春:吉林农业大学,2015.
[6]苏锐,张红.马齿苋黄酮抗氧化活性研究[J].安徽农业科学,2010,38(8):4068-4070.
[7]朱晓宦,吴向阳,仰榴青,等.马齿苋粗多糖的提取及清除羟自由基活性作用[J].江苏大学学报(医学版),2007,17(1):57-60.
[8]罗新星.金花茶美白功能研究及其产品制备[D].大连:大连理工大学,2015.
[9]陈文伟,肖丽英.8种中草药对耐药金葡菌的最小抑菌质量浓度检测[J].广东药学,2005,15(3):72-73.
[10]贾之慎,邬建敏,唐孟成.比色法测定Fenton反应产生的羟自由基[J].生物化学与生物物理进展,1996(2):184-186,193.
[11]董秀玥.配对t检验与成组t检验优选方法研究[J].数理医药学杂志,2010,23(1):11-14.
[12]Nandhini M,Sivanandam S N.An improved predictive association rule based classifier using gain ratio and T-test for health care data diagnosis[J].Sadhana,2015,40(9):1683-1699.
[13]区梓聪,何秋星,陈佩,等.8种中药提取物在膏霜类化妆品中的抑菌效能研究[J].日用化学工业,2017,47(3):148-152,158.
[14]Kawasaki M,Toyoda M,Teshima R,et al.In vitro antiallergic activity of flavonoids in histamine release assay using rat basophilic leukemia(RBL-2H3)cells[J].J.Food Hyg.Soc.Japan,1994,35:497-503.
[15]陆泽安,郑赛华,马来记,等.绵羊红细胞溶血试验用于化妆品眼刺激试验的动物替代方法研究[J].日用化学品科学,2013,36(1):23-27.
[16]敖华英,程树军,谈伟君.不同哺乳动物红细胞溶血替代眼刺激实验的比较[J].中国组织工程研究,2012,16(12):2163-2166.