利谷隆致SD雄性子代大鼠的生殖发育毒性影响
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摘要
目的:探索利谷隆致SD雄性子代大鼠的生殖发育毒性影响。方法:分别于SD大鼠孕期妊娠期连续灌服花生油和利谷隆染毒后,获得子鼠。实验分对照组和利谷隆组,子代雄性大鼠生长至性成熟后,行精子分析,取生殖组织进行HE染色,睾丸行透射电镜观察。结果:与对照组比较,利谷隆组子代雄性小鼠精子数量显著降低(P<0.01)。利谷隆组睾丸组织经HE染色发现部分生精小管结构破坏,生精细胞及精子溢出,电镜发现间质细胞线粒体肿胀,内质网扩张。结论:利谷隆通过损伤睾丸组织影响SD雄性子代大鼠的生殖发育。
Objective: To explore the reproductive toxicity of linuron on F1 male rats. Method: Pregnant female rats were exposed to linuron and peanut oil everyday during the period of gestation day ( GD) ,and were randomly divided into linuron group and control group. After maturation,the semen from F1 male rats was analyzed and HE stain and transmission electron microscopy were used to detecte the tissue development of testicle,prostate,epididymis,spermatica from F1 male rats. Result: Semen analysis showed that in the linuron group,the number of sperm significantly decreased ( P < 0. 01) . There were some structural damage in seminiferous tubules,some spermatogenic cells and sperm overflowed and some plasma cells infiltrated in the testicle. Conclusion: Our results demonstrated that linuron inducing testicle development malformation,significant decrease of sperm and increase of sperm malformation in F1 male rats.
Objective: To explore the reproductive toxicity of linuron on F1 male rats. Method: Pregnant female rats were exposed to linuron and peanut oil everyday during the period of gestation day ( GD) ,and were randomly divided into linuron group and control group. After maturation,the semen from F1 male rats was analyzed and HE stain and transmission electron microscopy were used to detecte the tissue development of testicle,prostate,epididymis,spermatica from F1 male rats. Result: Semen analysis showed that in the linuron group,the number of sperm significantly decreased ( P < 0. 01) . There were some structural damage in seminiferous tubules,some spermatogenic cells and sperm overflowed and some plasma cells infiltrated in the testicle. Conclusion: Our results demonstrated that linuron inducing testicle development malformation,significant decrease of sperm and increase of sperm malformation in F1 male rats.
引文
1C E.Beste.Weed Science Society of America.Herbicide Handbook.5th ed.Champaign,Illinois:Weed Science Society of America,1983:286.
2Chen CD,Welsbie DS,Tran C,et al.Molecular determinants of resistance to antiandrogen therapy.Nat Med,2004,10(1):33-39.
3Kang IH,Kim HS,Shin JH,et al.Comparison of anti-androgenic activity of flutamide,vinclozolin,procymidone,linuron,and p,p'-DDE in rodent10-day Hershberger assay.Toxicology,2004,199(2-3):145-159.
4Lambright C,Ostby J,Bobseine K,et al.Cellular and molecular mecha-nisms of action of linuron:an antiandrogenic herbicide that produces repro-ductive malformations in male rats.Toxicol Sci,2000,56(2):389-399.
5McIntyre BS,Barlow NJ,Sar M,et al.Effects of in utero linuron exposure on rat Wolffian duct development.Reprod Toxicol,2002,16(2):131-139.
6芦冉,张波,张鑫,王东威.3,4-二氯苯胺对雄性大鼠精子顶体酶活性和顶体反应的影响.中国工业医学杂志,2008(2):99-101.
7芦冉.3,4-二氯苯胺对雄性大鼠生殖系统的毒性效应[D].首都师范大学,2008.
2Chen CD,Welsbie DS,Tran C,et al.Molecular determinants of resistance to antiandrogen therapy.Nat Med,2004,10(1):33-39.
3Kang IH,Kim HS,Shin JH,et al.Comparison of anti-androgenic activity of flutamide,vinclozolin,procymidone,linuron,and p,p'-DDE in rodent10-day Hershberger assay.Toxicology,2004,199(2-3):145-159.
4Lambright C,Ostby J,Bobseine K,et al.Cellular and molecular mecha-nisms of action of linuron:an antiandrogenic herbicide that produces repro-ductive malformations in male rats.Toxicol Sci,2000,56(2):389-399.
5McIntyre BS,Barlow NJ,Sar M,et al.Effects of in utero linuron exposure on rat Wolffian duct development.Reprod Toxicol,2002,16(2):131-139.
6芦冉,张波,张鑫,王东威.3,4-二氯苯胺对雄性大鼠精子顶体酶活性和顶体反应的影响.中国工业医学杂志,2008(2):99-101.
7芦冉.3,4-二氯苯胺对雄性大鼠生殖系统的毒性效应[D].首都师范大学,2008.