IL-33基因rs4740840G/A、rs16924159G/A多态性在广西黑衣壮儿童的分布特点
|
摘要
目的探讨IL-33基因rs4740840G/A、rs16924159G/A两位点的多态性在广西黑衣壮儿童中的分布特点及其与不同种族人群的比较。方法采用多重连接酶检测反应(improved multi-ligase detection reaction,im LDR)技术对251例受试者(黑衣壮儿童121人,汉族儿童130人)的IL-33基因rs4740840G/A、rs16924159G/A位点多态性进行基因分型。用统计学方法分析其分布频率及组间差异。结果 rs4740840G/A存在AA、AG、GG三种基因型,分布频率分别为21.5%、46.3%、32.2%。其各基因型及等位基因频率在广西黑衣壮儿童、汉族儿童中分布差异有统计学意义(P<0.05)。广西黑衣壮儿童基因型和等位基因频率与人类基因组计划公布的欧洲人相比差异有统计学意义(P<0.01),与北京汉族人、日本人、非洲黑人相比差异无统计学意义(P>0.05)。rs16924159G/A存在AA、AG、GG三种基因型,分布频率分别为5.8%、34.7%、59.5%。其各基因型及等位基因频率在广西黑衣壮儿童、汉族儿童之间差异有统计学意义(P<0.01)。广西黑衣壮儿童基因型及等位基因分布频率与人类基因组计划公布的北京汉族、非洲黑人相比差异有统计学意义(P<0.05),与日本人、欧洲人相比差异无统计学意义(P>0.05)。结论 IL-33基因rs4740840G/A、rs16924159G/A在广西黑衣壮儿童分布存在多态性,同时还存在着不同程度的种族和地区差异。
Objective To explore the distribution characteristics of rs4740840 G/A and rs16924159 G/A polymorphisms of interleukin-33( IL-33) gene in Black-clothes Zhuang children in Guangxi and its comparison with different ethnic groups.Methods The polymorphisms of rs4740840 G/A and rs16924159 G/A of IL-33 gene in 251 subjects( 121 Black-clothes Zhuang children and 130 Han children) was genotyped by improved multi-ligase detection reaction( im-LDR) technique.And distribution frequencies and differences were analyzed by statistical methods between groups.Results Three genotypes( AA,AG and GG) were found in rs4740840 G/A with frequency of 21.5%,46.3% and 32.2%,respectively.The genotype and allele frequencies of rs4740840 G/A showed statistically significant difference between Black-clothes Zhuang children and Han children in Guangxi( P<0.05).The frequency of genotypes and alleles in the Black-clothes Zhuang children in Guangxi was statistically different from that of the Europeans published by Human Genome Project( P<0.01),but it was not statistically different from those of Beijing Han,Japanese and African( P > 0. 05). Three genotypes( AA,AG and GG) were found in rs16924159 G/A with frequency of 5.8%,34.7% and 59.5%,respectively.The genotype and allele frequencies of 16924159 G/A showed statistically significant difference between Black-clothes Zhuang children and Han children in Guangxi( P< 0.01).The distribution frequency of genotypes and alleles in Black-clothes Zhuang children in Guangxi was statistically significant compared with those in Beijing Han and African published by the Human Genome Project( P<0.05),but there was no statistically significant difference between the Japanese and the Europeans( P>0.05).Conclusion The distribution of rs4740840 G/A and rs16924159 G/A of IL-33 gene shows polymorphisms in Black-clothes Zhuang children in Guangxi,which exists varying degrees of racial and regional differences
Objective To explore the distribution characteristics of rs4740840 G/A and rs16924159 G/A polymorphisms of interleukin-33( IL-33) gene in Black-clothes Zhuang children in Guangxi and its comparison with different ethnic groups.Methods The polymorphisms of rs4740840 G/A and rs16924159 G/A of IL-33 gene in 251 subjects( 121 Black-clothes Zhuang children and 130 Han children) was genotyped by improved multi-ligase detection reaction( im-LDR) technique.And distribution frequencies and differences were analyzed by statistical methods between groups.Results Three genotypes( AA,AG and GG) were found in rs4740840 G/A with frequency of 21.5%,46.3% and 32.2%,respectively.The genotype and allele frequencies of rs4740840 G/A showed statistically significant difference between Black-clothes Zhuang children and Han children in Guangxi( P<0.05).The frequency of genotypes and alleles in the Black-clothes Zhuang children in Guangxi was statistically different from that of the Europeans published by Human Genome Project( P<0.01),but it was not statistically different from those of Beijing Han,Japanese and African( P > 0. 05). Three genotypes( AA,AG and GG) were found in rs16924159 G/A with frequency of 5.8%,34.7% and 59.5%,respectively.The genotype and allele frequencies of 16924159 G/A showed statistically significant difference between Black-clothes Zhuang children and Han children in Guangxi( P< 0.01).The distribution frequency of genotypes and alleles in Black-clothes Zhuang children in Guangxi was statistically significant compared with those in Beijing Han and African published by the Human Genome Project( P<0.05),but there was no statistically significant difference between the Japanese and the Europeans( P>0.05).Conclusion The distribution of rs4740840 G/A and rs16924159 G/A of IL-33 gene shows polymorphisms in Black-clothes Zhuang children in Guangxi,which exists varying degrees of racial and regional differences
引文
[1]Schmitz J,Owyang A,Oldham E,et al.IL-33,an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines[J].Immunity,2005,23(5):479-490.
[2]Rostan O,Arshad MI,Piquet-Pellorce C,et al.Crucial and diverse role of the interleukin-33/ST2 axis in infectious diseases[J].Infect Immun,2015,83(5):1738-1748.
[3]蔡旭龙,林娜.白介素-9基因多态性及其血清水平与黑衣壮儿童哮喘发病的关系[J].广西医学,2017,39(1):15-17.
[4]Koca SS,Pehlivan Y,Kara M,et al.The IL-33 gene is related to increased susceptibility to systemic sclerosis[J].Rheumatol Int,2016,36(4):579-584.
[5]Yang JH,Wu FQ,Wen Q,et al.Association of IL33/ST2signal pathway gene polymorphisms with myocardial infarction in a Chinese Han population[J].J Huazhong Univ Sci Technolog Med Sci,2015,35(1):16-20.
[6]Guo J,Xiang Y,Peng YF,et al.The association of novel IL-33 polymorphisms with s IL-33 and risk of systemic lupus erythematosus[J].Mol Immunol,2016,77:1-7.
[7]Fan D,Ding N,Yang T,et al.Single nucleotide polymorphisms of the interleukin-33(IL-33)gene are associated with ankylosing spondylitis in Chinese individuals:a case-control pilot study[J].Scand J Rheumatol,2014,43(5):374-379.
[8]Tu X,Nie S,Liao Y,et al.The IL-33-ST2L pathway is associated with coronary artery disease in a Chinese Han population[J].Am J Hum Genet,2013,93(4):652-660.
[9]Charrad R,Kaabachi W,Berraies A,et al.IL-33 gene variants and protein expression in pediatric Tunisian asthmatic patients[J].Cytokine,2018,104:85-91.
[10]Zhong X,Liu MY,He M,et al.Association of interleukin-33 gene polymorphisms with susceptibility to late onset Alzheimer's disease:a meta-analysis[J].Neuropsychiatr Dis Treat,2017,13:2275-2284.
[11]Xu W,Liu Y,Ye D.Association between IL-33 Gene Polymorphisms(rs1929992,rs7044343)and Systemic Lupus Erythematosus in a Chinese Han Population[J].Immunol Invest,2016,45(7):575-583.
[12]Kordi-Tamandani DM,Bahrami AR,Sabbaghi-Ghale-No R,et al.Analysis of IL-33 gene polymorphism(rs11792633 C/T)and risk of schizophrenia[J].Mol Biol Res Commun,2016,5(1):45-48.
[13]Sun XL,Law BY,de Seabra RDI,et al.Pathogenesis of thromboangiitis obliterans:Gene polymorphism and immunoregulation of human vascular endothelial cells[J].Atherosclerosis,2017,265:258-265.
[14]Yue J,Tong Y,Xie L,et al.Genetic variant in IL-33 is associated with idiopathic recurrent miscarriage in Chinese Han population[J].Sci Rep,2016,6:23806.
[15]Ren X,Guo M,Liu C,et al.A Case-Control Study Indicates that no Association Exists Between Polymorphisms of IL-33 and IL-1RL1 and Preeclampsia[J].Cell Physiol Biochem,2016,38(4):1406-1414.
[16]Queiroz GA,Costa RS,Alcantara-Neves NM,et al.IL33and IL1RL1 variants are associated with asthma and atopy in a Brazilian population[J].Int J Immunogenet,2017,44(2):51-61.
[17]Angeles-Martínez J,Posadas-Sanchez R,Llorente L,et al.The rs7044343 Polymorphism of the Interleukin 33 Gene Is Associated with Decreased Risk of Developing Premature Coronary Artery Disease and Central Obesity,and Could Be Involved in Regulating the Production of IL-33[J].PLo S One,2017,12(1):e168828.
[18]郭静,向阳,黄华佗,等.广西人群中白介素-33基因rsl891385A/C、rs10975519C/T多态性的分布特点[J].南方医科大学学报,2016,36(7):964-968.
[19]郭静,向阳,彭友帆,等.广西人群中IL-33基因rs10975521A/T、rs1929992A/G多态性的分布特点[J].中国免疫学杂志,2016,32(6):849-852.
[20]吴晓静,钟丹妮,叶德志,等.广西黑衣壮族高胆红素血症新生儿UGT1A1基因突变分析[J].中国当代儿科杂志,2014,16(5):483-488.
[2]Rostan O,Arshad MI,Piquet-Pellorce C,et al.Crucial and diverse role of the interleukin-33/ST2 axis in infectious diseases[J].Infect Immun,2015,83(5):1738-1748.
[3]蔡旭龙,林娜.白介素-9基因多态性及其血清水平与黑衣壮儿童哮喘发病的关系[J].广西医学,2017,39(1):15-17.
[4]Koca SS,Pehlivan Y,Kara M,et al.The IL-33 gene is related to increased susceptibility to systemic sclerosis[J].Rheumatol Int,2016,36(4):579-584.
[5]Yang JH,Wu FQ,Wen Q,et al.Association of IL33/ST2signal pathway gene polymorphisms with myocardial infarction in a Chinese Han population[J].J Huazhong Univ Sci Technolog Med Sci,2015,35(1):16-20.
[6]Guo J,Xiang Y,Peng YF,et al.The association of novel IL-33 polymorphisms with s IL-33 and risk of systemic lupus erythematosus[J].Mol Immunol,2016,77:1-7.
[7]Fan D,Ding N,Yang T,et al.Single nucleotide polymorphisms of the interleukin-33(IL-33)gene are associated with ankylosing spondylitis in Chinese individuals:a case-control pilot study[J].Scand J Rheumatol,2014,43(5):374-379.
[8]Tu X,Nie S,Liao Y,et al.The IL-33-ST2L pathway is associated with coronary artery disease in a Chinese Han population[J].Am J Hum Genet,2013,93(4):652-660.
[9]Charrad R,Kaabachi W,Berraies A,et al.IL-33 gene variants and protein expression in pediatric Tunisian asthmatic patients[J].Cytokine,2018,104:85-91.
[10]Zhong X,Liu MY,He M,et al.Association of interleukin-33 gene polymorphisms with susceptibility to late onset Alzheimer's disease:a meta-analysis[J].Neuropsychiatr Dis Treat,2017,13:2275-2284.
[11]Xu W,Liu Y,Ye D.Association between IL-33 Gene Polymorphisms(rs1929992,rs7044343)and Systemic Lupus Erythematosus in a Chinese Han Population[J].Immunol Invest,2016,45(7):575-583.
[12]Kordi-Tamandani DM,Bahrami AR,Sabbaghi-Ghale-No R,et al.Analysis of IL-33 gene polymorphism(rs11792633 C/T)and risk of schizophrenia[J].Mol Biol Res Commun,2016,5(1):45-48.
[13]Sun XL,Law BY,de Seabra RDI,et al.Pathogenesis of thromboangiitis obliterans:Gene polymorphism and immunoregulation of human vascular endothelial cells[J].Atherosclerosis,2017,265:258-265.
[14]Yue J,Tong Y,Xie L,et al.Genetic variant in IL-33 is associated with idiopathic recurrent miscarriage in Chinese Han population[J].Sci Rep,2016,6:23806.
[15]Ren X,Guo M,Liu C,et al.A Case-Control Study Indicates that no Association Exists Between Polymorphisms of IL-33 and IL-1RL1 and Preeclampsia[J].Cell Physiol Biochem,2016,38(4):1406-1414.
[16]Queiroz GA,Costa RS,Alcantara-Neves NM,et al.IL33and IL1RL1 variants are associated with asthma and atopy in a Brazilian population[J].Int J Immunogenet,2017,44(2):51-61.
[17]Angeles-Martínez J,Posadas-Sanchez R,Llorente L,et al.The rs7044343 Polymorphism of the Interleukin 33 Gene Is Associated with Decreased Risk of Developing Premature Coronary Artery Disease and Central Obesity,and Could Be Involved in Regulating the Production of IL-33[J].PLo S One,2017,12(1):e168828.
[18]郭静,向阳,黄华佗,等.广西人群中白介素-33基因rsl891385A/C、rs10975519C/T多态性的分布特点[J].南方医科大学学报,2016,36(7):964-968.
[19]郭静,向阳,彭友帆,等.广西人群中IL-33基因rs10975521A/T、rs1929992A/G多态性的分布特点[J].中国免疫学杂志,2016,32(6):849-852.
[20]吴晓静,钟丹妮,叶德志,等.广西黑衣壮族高胆红素血症新生儿UGT1A1基因突变分析[J].中国当代儿科杂志,2014,16(5):483-488.