海洋环境中细菌耐药性研究进展
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摘要
细菌对抗生素的耐药性作为一个潜在威胁健康的问题已成为严重的全球性公共卫生问题,细菌耐药性持续存在是在相当长的时间内必须面对和认真研究解决的难题。海洋环境也已逐步成为耐药性细菌的重要储存库。因此,本文针对海洋环境中细菌耐药性这一问题,结合国内外最新文献,对海洋环境中细菌耐药性研究进展进行了综述,简单介绍了细菌耐药性在海洋环境中的产生原因、流行现状、传播途径及影响因素,并对未来重点研究方向进行了展望。
Antimicrobial resistance in pathogenic bacteria has become a major threat to human health worldwide and is also a difficult obstacle to overcome in treating resistant bacterial infections. Marine environment has been a reservoir for antimicrobial resistant strain. Therefore, this article reviews the research progress of bacterial resistance in marine environment by summarizing and comparing with many relative studies. And this review primarily introduces the emergence, dissemission, antibiotic resistance mechanism and the future research directions of bacterial resistance in the marine environment.
Antimicrobial resistance in pathogenic bacteria has become a major threat to human health worldwide and is also a difficult obstacle to overcome in treating resistant bacterial infections. Marine environment has been a reservoir for antimicrobial resistant strain. Therefore, this article reviews the research progress of bacterial resistance in marine environment by summarizing and comparing with many relative studies. And this review primarily introduces the emergence, dissemission, antibiotic resistance mechanism and the future research directions of bacterial resistance in the marine environment.
引文
[1]Marti E,Variatza E,Balcazar J L.The role of aquatic ecosystems as reservoirs of antibiotic resistance[J].Trends Microbil,2014,22(1):36-41.
[2]Centers for Disease Control and Prevention(CDC)Biggest threats[EB/OL].Available athttps://www.cdc.gov/drugresistance/biggest_threats.html.
[3]朱永官,陈青林,苏建强,等.环境中抗生素与抗性基因组的研究[J].科学观察,2017,(6):60-62.
[4]Turgeon P,Michel P,Levallois P,et al.Agroenvironmental determinants associated with the presence of antimicrobialresistant Escherichia coli in beach waters in Quebec,Canada[J].Zoonoses Public Hlth,2011,58(6):432-439.
[5]Yang J,Feng J.Marine sediment bacteria harbor antibiotic resistance genes highly similar to those found in human pathogens[J].Microb Ecol,2013,65(4):975-981.
[6]Kumar S,Tripathi V R,Garg S K.Antibiotic resistance and genetic diversity in water-borne;Enterobacteriaceae isolates from recreational and drinking water sources[J].Int J Environ S,2013,10(4):789-798.
[7]邓玉,倪福全.水环境中抗生素残留及其危害[J].南水北调与水利科技,2011,9(3):96-100.
[8]刘伟,王慧,陈小军,等.抗生素在环境中降解的研究进展[J].动物医学进展,2009,30(3):89-94.
[9]彭金菊,马驿,杨明,等.诺氟沙星诱导水生细菌产生耐药性的最低选择浓度的调查[J].中国兽药杂志,2009,43(6):12-14.
[10]Chen B,Yang Y,Liang X,et al.Metagenomic profiles of antibiotic resistance genes(ARGs)between human impacted estuary and deep ocean sediments[J].Environ Sci Technol,2013,47(22):12753-12760.
[11]佟娟,魏源送.污水处理厂削减耐药菌与抗性基因的研究进展[J].环境科学学报,2012,32(11):2650-2659.
[12]Cheesanford J C,Aminov R I,Krapac I J,et al.Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities[J].Appl Environ Microbiol,2001,67(4):1494-1502.
[13]Burridge L,Weis J S,Cabello F,et al.Chemical use in salmon aquaculture:A review of current practices and possible environmental effects[J].Aquaculture,2010,306(1):7-23.
[14]Romero J,FeijoóC G,Navarrete P.Antibiotics in aquaculture-use,abuse and alternatives[M].Health and Environment in Aquaculture.IntechOpen,2012.
[15]Cabello F C.Heavy use of prophylactic antibiotics in aquaculture:a growing problem for human and animal health and for the environment[J].Environ Microbiol,2006,8(7):1137-1144.
[16]Sarmah A K,Meyer M T,Boxall A B.A global perspective on the use,sales,exposure pathways,occurrence,fate and effects of veterinary antibiotics(VAs)in the environment[J].Chemosphere,2006,65(5):725-759.
[17]Kümmerer K.Antibiotics in the aquatic environment-a review-part II[J].Chemosphere,2009,75(4):435-441.
[18]Szekeres E,Baricz A,Chiriac C M,et al.Abundance of antibiotics,antibiotic resistance genes and bacterial community composition in wastewater effluents from different Romanian hospitals[J].Environ Pollut,2017,225:304-315.doi:10.1016/j.envpol.2017.01.054.
[19]蒋魁,徐力文,苏友禄,等.2012年-2014年南海海水养殖鱼类病原菌哈维弧菌分离株的耐药性分析[J].南方水产科学,2016,12(6):99-107.
[20]龙文芳,李天娇,黄飞羽,等.海水浴场革兰阴性菌的检测及耐药性研究[J].环境与健康杂志,2016,33(3):226-228.
[21]王玥,苏洁,明红霞,等.浴场海水中大肠埃希菌耐药性及其耐药基因研究[J].海洋学报,2015,37(12):123-128.
[22]Ghaderpour A,Ho W S,Chew L L,et al.Diverse and abundant multi-drug resistant E.coli in Matang mangrove estuaries,Malaysia[J].Front Microbiol,2015,6(1):47.
[23]Odeyemi O A,Stratev D.Occurrence of antimicrobial resistant or pathogenic Vibrio parahaemolyticus in seafood.A mini review[J].Revue De Médecine Vétérinaire,2016,167(3-4):93-98.
[24]Odeyemi O A,Ahmad A.Population dynamics,antibiotics resistance and biofilm formation of aeromonas,and vibrio,species isolated from aquatic sources in Northern Malaysia[J].Microb Pathogenesis,2017,103:178-185.
[25]Tanil G B,Radu S,Nishibuchi M,et al.Characterization of Vibrio parahaemolyticus isolated from coastal seawater in Peninsular Malaysia[J].Se Asian J Trop Med,2005,36(4):940.
[26]You K G,Bong C W,Lee C W.Antimicrobial resistance in bacteria isolated from tropical coastal waters of Peninsular Malaysia[J].Malays J Sci,2012,31(2):111-120.
[27]You K G,Bong C W,Lee C W.Antibiotic resistance and plasmid profiling of Vibrio spp.in tropical waters of Peninsular Malaysia[J].Environ Monit Assess,2016,188(3):171.doi:10.1007/s10661-016-5163-0.
[28]Alves M S,Pereira A,Araújo S M,et al.Seawater is a reservoir of multi-resistant Escherichia coli,including strains hosting plasmid-mediated quinolones resistance and extended-spectrum beta-lactamases genes[J].Front Microbiol,2014,5(426):426.doi:10.3389/fmicb.2014.00426.
[29]Hatosy S M,Martiny A C.The ocean as a global reservoir of antibiotic resistance genes[J].Appl Environ Microbiol,2015,81(21):7593-7599.
[30]Zhu Y G,Zhao Y,Li B,et al.Continental-scale pollution of estuaries with antibiotic resistance genes[J].Nat Microbiol,2017,2:16270.
[31]邹世春,朱春敬,贺竹梅,等.北江河水中抗生素抗性基因污染初步研究[J].生态毒理学报,2009,4(5):655-660.
[32]Niu Z G,Zhang K,Zhang Y.Occurrence and distribution of antibiotic resistance genes in the coastal area of the Bohai Bay,China[J].Mar Pollut Bull,2016,107(1):245-250.
[33]Gao H,Zhang L,Lu Z,et al.Complex migration of antibiotic resistance in natural aquatic environments[J].Environ Pollut,2017,232:1-9.
[34]Gao Q,Li Y,Qi Z,et al.Diverse and abundant antibiotic resistance genes from mariculture sites of China's coastline[J].Sci Total Environ,2018,630:117-125.
[35]Nathani N M,Mootapally C,Dave B P.Antibiotic resistance genes allied to the pelagic sediment microbiome in the Gulf of Khambhat and Arabian Sea[J].Sci Total Environ,2018,653:446-454.
[36]Shao S,Hu Y,Cheng J,et al.Research progress on distribution,migration,transformation of antibioticsand antibiotic resistance genes(ARGs)in aquatic environment[J].Crit Rev Biotechnol,2018,38(8):1195-1208.
[37]Tenover F C.Mechanisms of antimicrobial resistance in bacteria[J].Am J Med,2006,119(6):S3-S10.
[38]Courvalin P.Transfer of antibiotic resistance genes between Gram-positive and Gram-negative bacteria[J].Antimicrob Agents Chemother,1994,38(7):1447-1451.
[39]蓝惠华,张玲,王厚照.水平基因转移介导抗菌素耐药性传播机制的研究进展[J].微生物学杂志,2017,37(4):93-97.
[40]赵莎.大连付家庄海水浴场中大肠埃希菌耐药性及质粒介导的耐药性传递研究[D].大连:大连海洋大学,2017.
[41]de Vries J,Wackernagel W.Microbial horizontal gene transfer and the DNA release from transgenic crop plants[J].Plant Soil,2005,266(1-2):91-104.
[42]张宁,李淼,刘翔.土壤中抗生素抗性基因的分布及迁移转化[J].中国环境科学,2018,38(7):2609-2617.
[43]Karl D M,Bailiff M D.The measurement and distribution of dissolved nucleic acids in aquatic environments[J].Limnol Oceanogr,1989,34(3):543-558.
[44]Lang A S,Beatty J T.Genetic analysis of a bacterial genetic exchange element:The gene transfer agent of Rhodobacter capsulatus[J].P Natl Acad Sci USA,2000,97(2):859-864.
[45]Zinder N D,Lederberg J.Genetic exchange in Salmonella[J].J Bacteriol,1952,64(5):679-699.
[46]蔡海元.基因转移因子--一类在海洋中广泛存在的介导水平基因转移的新型生物因子[J].微生物学报,2012,52(1):12-21.
[47]Jiang S C,Paul J H.Gene transfer by transduction in the marine environment[J].Appl Environ Microb,1998,64(8):2780-2787.
[48]Marrs B.Genetic recombination in Rhodopseudomonas capsulata[J].P Natl Acad Sci USA,1974,71(3):971-973.
[49]Paul J H.Prophages in marine bacteria:dangerous molecular time bombs or the key to survival in the seas[J].Isme J,2008,2(6):579-589.
[50]Biers E J,Wang K,Pennington C,et al.Occurrence and expression of gene transfer agent genes in marine bacterioplankton[J].Appl Environ Microbiol,2008,74(10):2933-2939.
[51]Pruden A,Pei R,Storteboom H,et al.Antibiotic resistance genes as emerging contaminants:Studies in Northern Colorado[J].Environ Sci Technol,2006,40(23):7445.
[52]Su H C,Ying G G,Tao R,et al.Class 1 and 2 integrons,sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River,South China[J].Environ Pollut,2012,169(15):42-49.
[53]Pereira A,Santos A,Tac?o M,et al.Genetic diversity and antimicrobial resistance of Escherichia coli from Tagus estuary(Portugal)[J].Sci Total Environ,2013,461-462(7):65-71.
[54]Garner E,Benitez R,Von W E,et al.Stormwater loadings of antibiotic resistance genes in an urban stream[J].Water Res,2017,123:144-152.
[55]Ahmed W,Zhang Q,Lobos A,et al.Precipitation influences pathogenic bacteria and antibiotic resistance gene abundance in storm drain outfalls in coastal sub-tropical waters[J].Environ Int,2018,116:308-318.
[56]Lu Z,Na G,Gao H,et al.Fate of sulfonamide resistance genes in estuary environment and effect of anthropogenic activities[J].Sci Total Environ,2015,527-528:429-438.
[57]Yang C W,Chang Y T,Chao W L,et al.An investigation of total bacterial communities,culturable antibiotic-resistant bacterial communities and integrons in the river water environments of Taipei city[J].J Hazard Mater,2014,277(4):159-168.
[58]郭业彬,舒为群,常晓松,等.温度与pH值对长江水系产超广谱β-内酰胺酶大肠埃希菌耐药基因转移影响分析[J].微生物学杂志,2010,30(5):29-35.
[59]何良英.典型畜禽养殖环境中抗生素耐药基因的污染特征与扩散机理研究[D].北京:中国科学院大学,2016.
[60]Frost I,Smith W,Mitri S,et al.Cooperation,competition and antibiotic resistance in bacterial colonies[J].Isme J,2018,12(6):1582-1593.
[2]Centers for Disease Control and Prevention(CDC)Biggest threats[EB/OL].Available athttps://www.cdc.gov/drugresistance/biggest_threats.html.
[3]朱永官,陈青林,苏建强,等.环境中抗生素与抗性基因组的研究[J].科学观察,2017,(6):60-62.
[4]Turgeon P,Michel P,Levallois P,et al.Agroenvironmental determinants associated with the presence of antimicrobialresistant Escherichia coli in beach waters in Quebec,Canada[J].Zoonoses Public Hlth,2011,58(6):432-439.
[5]Yang J,Feng J.Marine sediment bacteria harbor antibiotic resistance genes highly similar to those found in human pathogens[J].Microb Ecol,2013,65(4):975-981.
[6]Kumar S,Tripathi V R,Garg S K.Antibiotic resistance and genetic diversity in water-borne;Enterobacteriaceae isolates from recreational and drinking water sources[J].Int J Environ S,2013,10(4):789-798.
[7]邓玉,倪福全.水环境中抗生素残留及其危害[J].南水北调与水利科技,2011,9(3):96-100.
[8]刘伟,王慧,陈小军,等.抗生素在环境中降解的研究进展[J].动物医学进展,2009,30(3):89-94.
[9]彭金菊,马驿,杨明,等.诺氟沙星诱导水生细菌产生耐药性的最低选择浓度的调查[J].中国兽药杂志,2009,43(6):12-14.
[10]Chen B,Yang Y,Liang X,et al.Metagenomic profiles of antibiotic resistance genes(ARGs)between human impacted estuary and deep ocean sediments[J].Environ Sci Technol,2013,47(22):12753-12760.
[11]佟娟,魏源送.污水处理厂削减耐药菌与抗性基因的研究进展[J].环境科学学报,2012,32(11):2650-2659.
[12]Cheesanford J C,Aminov R I,Krapac I J,et al.Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities[J].Appl Environ Microbiol,2001,67(4):1494-1502.
[13]Burridge L,Weis J S,Cabello F,et al.Chemical use in salmon aquaculture:A review of current practices and possible environmental effects[J].Aquaculture,2010,306(1):7-23.
[14]Romero J,FeijoóC G,Navarrete P.Antibiotics in aquaculture-use,abuse and alternatives[M].Health and Environment in Aquaculture.IntechOpen,2012.
[15]Cabello F C.Heavy use of prophylactic antibiotics in aquaculture:a growing problem for human and animal health and for the environment[J].Environ Microbiol,2006,8(7):1137-1144.
[16]Sarmah A K,Meyer M T,Boxall A B.A global perspective on the use,sales,exposure pathways,occurrence,fate and effects of veterinary antibiotics(VAs)in the environment[J].Chemosphere,2006,65(5):725-759.
[17]Kümmerer K.Antibiotics in the aquatic environment-a review-part II[J].Chemosphere,2009,75(4):435-441.
[18]Szekeres E,Baricz A,Chiriac C M,et al.Abundance of antibiotics,antibiotic resistance genes and bacterial community composition in wastewater effluents from different Romanian hospitals[J].Environ Pollut,2017,225:304-315.doi:10.1016/j.envpol.2017.01.054.
[19]蒋魁,徐力文,苏友禄,等.2012年-2014年南海海水养殖鱼类病原菌哈维弧菌分离株的耐药性分析[J].南方水产科学,2016,12(6):99-107.
[20]龙文芳,李天娇,黄飞羽,等.海水浴场革兰阴性菌的检测及耐药性研究[J].环境与健康杂志,2016,33(3):226-228.
[21]王玥,苏洁,明红霞,等.浴场海水中大肠埃希菌耐药性及其耐药基因研究[J].海洋学报,2015,37(12):123-128.
[22]Ghaderpour A,Ho W S,Chew L L,et al.Diverse and abundant multi-drug resistant E.coli in Matang mangrove estuaries,Malaysia[J].Front Microbiol,2015,6(1):47.
[23]Odeyemi O A,Stratev D.Occurrence of antimicrobial resistant or pathogenic Vibrio parahaemolyticus in seafood.A mini review[J].Revue De Médecine Vétérinaire,2016,167(3-4):93-98.
[24]Odeyemi O A,Ahmad A.Population dynamics,antibiotics resistance and biofilm formation of aeromonas,and vibrio,species isolated from aquatic sources in Northern Malaysia[J].Microb Pathogenesis,2017,103:178-185.
[25]Tanil G B,Radu S,Nishibuchi M,et al.Characterization of Vibrio parahaemolyticus isolated from coastal seawater in Peninsular Malaysia[J].Se Asian J Trop Med,2005,36(4):940.
[26]You K G,Bong C W,Lee C W.Antimicrobial resistance in bacteria isolated from tropical coastal waters of Peninsular Malaysia[J].Malays J Sci,2012,31(2):111-120.
[27]You K G,Bong C W,Lee C W.Antibiotic resistance and plasmid profiling of Vibrio spp.in tropical waters of Peninsular Malaysia[J].Environ Monit Assess,2016,188(3):171.doi:10.1007/s10661-016-5163-0.
[28]Alves M S,Pereira A,Araújo S M,et al.Seawater is a reservoir of multi-resistant Escherichia coli,including strains hosting plasmid-mediated quinolones resistance and extended-spectrum beta-lactamases genes[J].Front Microbiol,2014,5(426):426.doi:10.3389/fmicb.2014.00426.
[29]Hatosy S M,Martiny A C.The ocean as a global reservoir of antibiotic resistance genes[J].Appl Environ Microbiol,2015,81(21):7593-7599.
[30]Zhu Y G,Zhao Y,Li B,et al.Continental-scale pollution of estuaries with antibiotic resistance genes[J].Nat Microbiol,2017,2:16270.
[31]邹世春,朱春敬,贺竹梅,等.北江河水中抗生素抗性基因污染初步研究[J].生态毒理学报,2009,4(5):655-660.
[32]Niu Z G,Zhang K,Zhang Y.Occurrence and distribution of antibiotic resistance genes in the coastal area of the Bohai Bay,China[J].Mar Pollut Bull,2016,107(1):245-250.
[33]Gao H,Zhang L,Lu Z,et al.Complex migration of antibiotic resistance in natural aquatic environments[J].Environ Pollut,2017,232:1-9.
[34]Gao Q,Li Y,Qi Z,et al.Diverse and abundant antibiotic resistance genes from mariculture sites of China's coastline[J].Sci Total Environ,2018,630:117-125.
[35]Nathani N M,Mootapally C,Dave B P.Antibiotic resistance genes allied to the pelagic sediment microbiome in the Gulf of Khambhat and Arabian Sea[J].Sci Total Environ,2018,653:446-454.
[36]Shao S,Hu Y,Cheng J,et al.Research progress on distribution,migration,transformation of antibioticsand antibiotic resistance genes(ARGs)in aquatic environment[J].Crit Rev Biotechnol,2018,38(8):1195-1208.
[37]Tenover F C.Mechanisms of antimicrobial resistance in bacteria[J].Am J Med,2006,119(6):S3-S10.
[38]Courvalin P.Transfer of antibiotic resistance genes between Gram-positive and Gram-negative bacteria[J].Antimicrob Agents Chemother,1994,38(7):1447-1451.
[39]蓝惠华,张玲,王厚照.水平基因转移介导抗菌素耐药性传播机制的研究进展[J].微生物学杂志,2017,37(4):93-97.
[40]赵莎.大连付家庄海水浴场中大肠埃希菌耐药性及质粒介导的耐药性传递研究[D].大连:大连海洋大学,2017.
[41]de Vries J,Wackernagel W.Microbial horizontal gene transfer and the DNA release from transgenic crop plants[J].Plant Soil,2005,266(1-2):91-104.
[42]张宁,李淼,刘翔.土壤中抗生素抗性基因的分布及迁移转化[J].中国环境科学,2018,38(7):2609-2617.
[43]Karl D M,Bailiff M D.The measurement and distribution of dissolved nucleic acids in aquatic environments[J].Limnol Oceanogr,1989,34(3):543-558.
[44]Lang A S,Beatty J T.Genetic analysis of a bacterial genetic exchange element:The gene transfer agent of Rhodobacter capsulatus[J].P Natl Acad Sci USA,2000,97(2):859-864.
[45]Zinder N D,Lederberg J.Genetic exchange in Salmonella[J].J Bacteriol,1952,64(5):679-699.
[46]蔡海元.基因转移因子--一类在海洋中广泛存在的介导水平基因转移的新型生物因子[J].微生物学报,2012,52(1):12-21.
[47]Jiang S C,Paul J H.Gene transfer by transduction in the marine environment[J].Appl Environ Microb,1998,64(8):2780-2787.
[48]Marrs B.Genetic recombination in Rhodopseudomonas capsulata[J].P Natl Acad Sci USA,1974,71(3):971-973.
[49]Paul J H.Prophages in marine bacteria:dangerous molecular time bombs or the key to survival in the seas[J].Isme J,2008,2(6):579-589.
[50]Biers E J,Wang K,Pennington C,et al.Occurrence and expression of gene transfer agent genes in marine bacterioplankton[J].Appl Environ Microbiol,2008,74(10):2933-2939.
[51]Pruden A,Pei R,Storteboom H,et al.Antibiotic resistance genes as emerging contaminants:Studies in Northern Colorado[J].Environ Sci Technol,2006,40(23):7445.
[52]Su H C,Ying G G,Tao R,et al.Class 1 and 2 integrons,sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River,South China[J].Environ Pollut,2012,169(15):42-49.
[53]Pereira A,Santos A,Tac?o M,et al.Genetic diversity and antimicrobial resistance of Escherichia coli from Tagus estuary(Portugal)[J].Sci Total Environ,2013,461-462(7):65-71.
[54]Garner E,Benitez R,Von W E,et al.Stormwater loadings of antibiotic resistance genes in an urban stream[J].Water Res,2017,123:144-152.
[55]Ahmed W,Zhang Q,Lobos A,et al.Precipitation influences pathogenic bacteria and antibiotic resistance gene abundance in storm drain outfalls in coastal sub-tropical waters[J].Environ Int,2018,116:308-318.
[56]Lu Z,Na G,Gao H,et al.Fate of sulfonamide resistance genes in estuary environment and effect of anthropogenic activities[J].Sci Total Environ,2015,527-528:429-438.
[57]Yang C W,Chang Y T,Chao W L,et al.An investigation of total bacterial communities,culturable antibiotic-resistant bacterial communities and integrons in the river water environments of Taipei city[J].J Hazard Mater,2014,277(4):159-168.
[58]郭业彬,舒为群,常晓松,等.温度与pH值对长江水系产超广谱β-内酰胺酶大肠埃希菌耐药基因转移影响分析[J].微生物学杂志,2010,30(5):29-35.
[59]何良英.典型畜禽养殖环境中抗生素耐药基因的污染特征与扩散机理研究[D].北京:中国科学院大学,2016.
[60]Frost I,Smith W,Mitri S,et al.Cooperation,competition and antibiotic resistance in bacterial colonies[J].Isme J,2018,12(6):1582-1593.