白花延龄草水提液对四氯化碳致小鼠肝纤维化的保护作用
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摘要
目的探讨白花延龄草水提液对肝纤维化作用及其作用机制。方法腹腔注射四氯化碳(CCl_4)构建雄性C57鼠肝纤维化模型,给药A组(白花延龄草水提液0. 5 g/kg)、给药B组(白花延龄草水提液1 g/kg)、阳性对照组(扶正化瘀胶囊0. 75 g/kg)分别进行灌胃给药,采用生化方法检测血清中的丙氨酸氨基转移酶(ALT)和天门冬酸氨基转移酶(AST)及肝脏组织中的超氧化物歧化酶(SOD)、脂质氧化终产物丙二醛(MDA)含量;苏木精-伊红(HE)染色法检测肝纤维化的变化;蛋白质印迹(Western Blot)法检测转化生长因子-β(TGF-β)蛋白表达水平。结果给药组和阳性对照组均能显著抑制CCl_4诱导的小鼠肝纤维化程度,与模型组相比,给药组和阳性对照组血清ALT,AST水平及肝脏组织中MDA水平明显降低(P <0. 01),SOD显著提高(P <0. 01);与模型组相比,给药组和阳性对照组TGF-β蛋白表达具有显著的抑制作用(P <0. 01)。结论白花延龄草水提液对肝纤维化有显著的改善作用。
Objective To investigate the protective effect of water extract of Trillium kamtschaticum on liver fibrosis. Methods The liver fibrosis model of male C57 mice was established by intraperitoneal injection of CCl_4. Group A(0. 5 g/kg water extract of Trillium kamtschaticum) and group B( 1 g/kg water extract of Trillium kamtschaticum) and positive control group( 0. 75 g/kg Fuzheng Huayu Capsules) were given intragastric administration respectively. The levels of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) in serum and the content of superoxide dismutase( SOD) and malondialdehyde( MDA) in liver tissue were detected by biochemical method. The change of liver fibrosis was measured by HE stain method. The expression level of transforming growth factor-β( TGF-β)protien was detected by Western Blot method. Results Both the drug group and the positive control group could significantly inhibit CCl_4-induced liver fibrosis in mice. Compared with the fibrosis model group,the levels of ALT,AST in serum and MDA in liver tissue in the drug group and the positive control group were significantly lower( P < 0. 01),while the levels of SOD were significantly higher( P < 0. 01). Compared with the model group,the expression level of TGF-β protein was significantly inhibited in the drug group and the positive control group( P < 0. 01). Conclusion The water extract of Trillium kamtschaticum has significant effect on improving liver fibrosis.
Objective To investigate the protective effect of water extract of Trillium kamtschaticum on liver fibrosis. Methods The liver fibrosis model of male C57 mice was established by intraperitoneal injection of CCl_4. Group A(0. 5 g/kg water extract of Trillium kamtschaticum) and group B( 1 g/kg water extract of Trillium kamtschaticum) and positive control group( 0. 75 g/kg Fuzheng Huayu Capsules) were given intragastric administration respectively. The levels of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) in serum and the content of superoxide dismutase( SOD) and malondialdehyde( MDA) in liver tissue were detected by biochemical method. The change of liver fibrosis was measured by HE stain method. The expression level of transforming growth factor-β( TGF-β)protien was detected by Western Blot method. Results Both the drug group and the positive control group could significantly inhibit CCl_4-induced liver fibrosis in mice. Compared with the fibrosis model group,the levels of ALT,AST in serum and MDA in liver tissue in the drug group and the positive control group were significantly lower( P < 0. 01),while the levels of SOD were significantly higher( P < 0. 01). Compared with the model group,the expression level of TGF-β protein was significantly inhibited in the drug group and the positive control group( P < 0. 01). Conclusion The water extract of Trillium kamtschaticum has significant effect on improving liver fibrosis.
引文
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[11]唐静,戴立里,呙琳琳,等.肝纤维化中丹参素对TGF-β1/Smads/ERK信号通路的影响及其相互关系[J].第三军医大学学报,2011,33(11):1159-1164.
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[13]邓家刚,周程艳,郑作文.黄根醇提物对肝纤维化大鼠肝组织中TGF-β1表达及COLI、COLⅢ水平的影响[J].北京中医药大学学报,2009,32(1):42-44.
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[15]王付,尚立芝,苗小玲,等.四逆散加味对肝纤维化大鼠肝功能、肝纤维化指标及病理变化的影响[J].中国实验方剂学杂志,2012,18(5):177-180.
[16]王永林.肝纤维化的病因病机与中医药治疗初探[J].现代中西医结合杂志,2011,20(36):4693-4694.
[2]胡明政,秦春宏.肝纤维化发病机制的研究进展[J].西南军医,2017,19(2):155-157.
[3]BREITHOPF K,HAAS S,WIERCINSKA E,et al.AntiTGF-beta strategies for the treatment of chronic liver disease[J].Alcohol Clin Exp Res,2005,29(Suppl 11):121S-131S.
[4]代东雪,潘志,唐成芳,等.消活络方对肝纤维化大鼠肝组织-SMA和TGF-1表达的影响[J].吉林中医药,2017,37(2):162-166.
[5]谢激扬.四逆散加减对酒精性肝纤维化患者TGF-β、肝纤维化指标及肝功能指标的影响[J].中华中医药学刊,2017,35(3):748-751.
[6]程敏,郑勇,陈卫刚,等.外源性硫化氢对肝星状细胞TGF-β1表达的影响[J].山东医药,2017,57(8):29-31.
[7]《全国中草药汇编》编写组.全国中草药汇编(上册)[M].第2版.北京:人民卫生出版社,1998:225-226.
[8]姜英,商云帅,刘继永.白花延龄草中8种无机营养元素测定分析[J].特产研究,2015,37(4):36-37.
[9]罗婧,赵卉,陈雪,等.不同采收期白花延龄草根茎中脂肪酸的GC-MS分析[J].中国林副特产,2015,138(5):16-20.
[10]刘继永,邵财,张浩,等.吉林省蛟河市濒危药用植物白花延龄草资源调查[J].中国林副特产,2014,132(5):73-75.
[11]唐静,戴立里,呙琳琳,等.肝纤维化中丹参素对TGF-β1/Smads/ERK信号通路的影响及其相互关系[J].第三军医大学学报,2011,33(11):1159-1164.
[12]曾建国,肖俐,王宇红,等.博落回提取物对实验性肝纤维化的防治作用[J].中国实验方剂学杂志,2012,18(1):134-150.
[13]邓家刚,周程艳,郑作文.黄根醇提物对肝纤维化大鼠肝组织中TGF-β1表达及COLI、COLⅢ水平的影响[J].北京中医药大学学报,2009,32(1):42-44.
[14]赵宗豪,梅俏,吴军,等.N-乙酰半胱氨酸保护大鼠肝纤维化的机制初步研究[J].安徽医学,2010,31(1):5-6.
[15]王付,尚立芝,苗小玲,等.四逆散加味对肝纤维化大鼠肝功能、肝纤维化指标及病理变化的影响[J].中国实验方剂学杂志,2012,18(5):177-180.
[16]王永林.肝纤维化的病因病机与中医药治疗初探[J].现代中西医结合杂志,2011,20(36):4693-4694.