胆南星中胆酸类成分含量测定及发酵前后含量比较
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摘要
目的:建立胆南星样品中猪胆酸、猪去氧胆酸和鹅去氧胆酸的含量测定方法,比较胆南星发酵前后这几种胆酸类成分的含量变化,为胆南星发酵原理、工艺规范化研究提供参考。方法:Waters C_(18)色谱柱(250 mm×4.6 mm,5μm),柱温为30℃;流动相为乙腈-0.5%冰乙酸溶液(60∶40);检测器:奥泰2000蒸发光散射检测器;流速:1.0 mL·min~(-1);漂移管温度:105℃,气体流速:3.0 mL·min~(-1)。结果:猪胆酸、猪去氧胆酸、鹅去氧胆酸均在定量范围内呈良好的线性关系,加样回收率分别为99.63%、100.04%、101.4%。结论:本方法操作简单,测定结果准确,胆南星发酵后猪胆酸、猪去氧胆酸和鹅去氧胆酸含量增加,可作为胆南星发酵程度及胆汁加入量的判定指标。
Objective:To develope an HPLC-ELSD method for determination of porcine cholic acid,hyodeoxycholic acid and chenodeoxycholic acid from Arisaema cum Bile,and compare the changes of the contents of these bile acids before and after the fermentation of Arisaema cum Bile,in order to provide a basis for the study of the principle and processing standardization of Arisaema cum Bile.Methods:Waters-C_(18)(250 mm×4.6 mm,5 μm)was employed and eluted at 30 ℃;with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid(60∶40);and the flow rate was 1.0 mL·min~(-1).The drift-tube temperature of ELSD was 105 ℃,and the flow rate of air was 3.0 mL·min~(-1).Results:The linearity of porcine cholic acid,hyodeoxycholic acid and chenodeoxycholic acid was good linear within the quantitative range,which recovery rates were 99.63%,100.04% and 101.4% respectively.Conclusion:The established method is simple and accurate,after the fermentation the acids increase,which can be used as the judgment of the fermentation degree.
Objective:To develope an HPLC-ELSD method for determination of porcine cholic acid,hyodeoxycholic acid and chenodeoxycholic acid from Arisaema cum Bile,and compare the changes of the contents of these bile acids before and after the fermentation of Arisaema cum Bile,in order to provide a basis for the study of the principle and processing standardization of Arisaema cum Bile.Methods:Waters-C_(18)(250 mm×4.6 mm,5 μm)was employed and eluted at 30 ℃;with a mobile phase consisting of acetonitrile-0.5% glacial acetic acid(60∶40);and the flow rate was 1.0 mL·min~(-1).The drift-tube temperature of ELSD was 105 ℃,and the flow rate of air was 3.0 mL·min~(-1).Results:The linearity of porcine cholic acid,hyodeoxycholic acid and chenodeoxycholic acid was good linear within the quantitative range,which recovery rates were 99.63%,100.04% and 101.4% respectively.Conclusion:The established method is simple and accurate,after the fermentation the acids increase,which can be used as the judgment of the fermentation degree.
引文
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[3] 赵启苗,单国顺,陈江宁,等.胆南星质量评价方法初探[J].中国实验方剂学杂志,2017,23(6):28-31.
[4] 陈江宁,单国顺,刘晓瑜,等.胆南星辅料成分分析及其清热作用[J].中国现代中药,2016,18(7):837-840.
[5] 蔡宇忆,叶永浩,杨丽莹,等.HPLC-ELSD法测定胆南星中胆酸类成分的含量[J].广东药学院学报,2016,32(3):311-314.
[6] 王光函,姜鸿,邹桂欣,等.HPLC-ELSD法测定胆南星中胆酸含量[J].亚太传统医药,2014,10(22):8-9.
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[8] 陈江宁.胆南星原辅料优选及发酵工艺研究[D].沈阳:辽宁中医药大学,2017.