一种高效的蝴蝶兰花梗诱导丛生芽体系
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摘要
为提高蝴蝶兰组织培养效率,分别以幼嫩花梗和已开花蝴蝶兰花梗腋芽为外植体,采用两种消毒剂HgCl2、NaClO,3种消毒方式0.1%HgCl2,10min;10%NaClO,10min;5%NaClO,10min为处理,以VW(大量)+MS(微量、铁盐、有机)+100g·L-1马铃薯+0.1g·L-1 Ga3(PO4)2+6mg·L-16-BA+0.2mg·L-1 NAA为诱导基本培养基;VW(大量)+MS(铁盐、有机、微量)+100g·L-1马铃薯+3mg·L-16-BA+0.2mg·L-1 NAA为增殖分化培养基;1/2MS+0.1mg·L-1 NAA为生根培养基诱导丛生芽,拟建立以蝴蝶兰花梗腋芽为外植体的高效丛生芽诱导体系。结果表明:在0.1%HgCl2,10min消毒方式下,幼嫩花梗腋芽的污染率为0,丛生芽诱导率为95.6%;增殖率为216%;在生根培养基1/2MS+0.1mg·L-1 NAA上生根率为95.6%,建立了高效的蝴蝶兰幼嫩花梗腋芽诱导丛生芽。
In order to improve the efficiency of tissue culture of Phalaenopsis,we used the axillary buds of young and flowering butterfly orchids as explants,and adopted two disinfectants(HgCl2 and NaClO),and three disinfection methods(0.1%HgCl2,10 min;10%NaClO,10 min;5% NaClO,10 min)to compare the disinfection effects.The mediums were as follows,VW(A large number of elements)+ MS(trace,iron,organic)+100 g·L-1 potato+0.1 g·L-1 Ga3(PO4)2+6 mg·L-16-BA+0.2 mg·L-1 NAA as the basic medium,VW(A large number of elements)+MS(trace,iron,and organic)+ 100 g·L-1 potato+ 3 mg·L-16-BA+ 0.2 mg·L-1 NAA as proliferation and differentiation medium,and 1/2 MS +0.1 mg·L-1 NAA induced clustered buds for rooting medium.The results showed that the induction rates of pollution rate was 0,the young shoots of axillary buds were 95.6%,the reproducibility rate was 216%,and the rooting rate of 1/2 MS+0.1 mg·L-1 NAA in the rooting medium was 95.6%,under 0.1% HgCl210 min disinfection method indicated the use of two kinds of medium,disinfection methods and induced proliferation and differentiation,was suitable for inducing shoots of axillary buds of young peduncle.
In order to improve the efficiency of tissue culture of Phalaenopsis,we used the axillary buds of young and flowering butterfly orchids as explants,and adopted two disinfectants(HgCl2 and NaClO),and three disinfection methods(0.1%HgCl2,10 min;10%NaClO,10 min;5% NaClO,10 min)to compare the disinfection effects.The mediums were as follows,VW(A large number of elements)+ MS(trace,iron,organic)+100 g·L-1 potato+0.1 g·L-1 Ga3(PO4)2+6 mg·L-16-BA+0.2 mg·L-1 NAA as the basic medium,VW(A large number of elements)+MS(trace,iron,and organic)+ 100 g·L-1 potato+ 3 mg·L-16-BA+ 0.2 mg·L-1 NAA as proliferation and differentiation medium,and 1/2 MS +0.1 mg·L-1 NAA induced clustered buds for rooting medium.The results showed that the induction rates of pollution rate was 0,the young shoots of axillary buds were 95.6%,the reproducibility rate was 216%,and the rooting rate of 1/2 MS+0.1 mg·L-1 NAA in the rooting medium was 95.6%,under 0.1% HgCl210 min disinfection method indicated the use of two kinds of medium,disinfection methods and induced proliferation and differentiation,was suitable for inducing shoots of axillary buds of young peduncle.
引文
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[10]章玉平,刘成运,胡鸿钧,等.蝴蝶兰无菌萌发技术的研究[J].植物科学学报,2004,22(1):82-86.
[2]谈静,张英杰,郭文姣,等.大花型红花蝴蝶兰花梗腋芽诱导的影响因素[J].中国农学通报,2016,32(16):87-92.
[3]陈传红,蔡奇英,管毕财,等.多效唑在生姜组织培养中的应用研究[C].第二届全国植物组织培养、脱毒快繁及工厂化生产学术研讨会,2004:43-44.
[4]潘丽晶,陈继敏,张妙彬,等.蝴蝶兰根内生细菌的分离及可分泌IAA细菌的筛选[J].中国农学通报,2014,30(16):148-152.
[5]陈桂敏,郑羽书,杨佩华,等.不同外植体对蝴蝶兰组培快繁的影响[J].中国园艺文摘,2013(11):15-16.
[6]张彩玲.蝴蝶兰花梗组织培养与快速繁殖的研究[J].中国林副特产,2010(5):42-44.
[7]柏峰,高如宾,柏杨,等.蝴蝶兰组培快繁的应用研究[J].唐山师范学院学报,2007,29(2):41-42.
[8]王仁睿,李明福,韩林波,等.蝴蝶兰组织培养体系的建立及其关键技术研究[J].中国农学通报,2010,26(10):197-201.
[9]吕永杰,李仕贵,周晓禾.观赏兰科植物组培快繁及遗传转化的研究进展[J].中国生物工程杂志,2003,23(10):42-46.
[10]章玉平,刘成运,胡鸿钧,等.蝴蝶兰无菌萌发技术的研究[J].植物科学学报,2004,22(1):82-86.
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