生长不同植物的刺槐树洞附生土中真菌群落组成的初步调查研究
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摘要
树洞是陆地生态系统特殊生境之一,目前对其研究报道不多。基于高通量测序,我们调查分析了贵州城市绿地一些生长有不同植物的刺槐树洞附生土样中真菌群落的组成。研究结果显示,在属水平上,植物为排风藤Solanum cathayanum、女贞Ligustrum lucidum、小藜Chenopodium serotinum、中国绣球Hydrangea chinensis、苦苣Cichorium endivia及牛膝Achyranthes bidentata的刺槐树洞附生土,其真菌群落优势属分别为绿僵菌属Metarhizium、粗糙孔菌属Trechispora、Fungi下一未鉴定属、团壳菌科Boliniaceae中一未定属、久浩酵母属Guehomyces及丛赤壳科Nectriaceae中一未定属,各优势属的相对多度分别为57.67%、42.96%、30.60%、86.45%、33.15%及12.26%。此外,样本中还存在许多昆虫及人类潜在病原菌和独特的真菌资源,值得进一步探索。
Tree hole is one special habitat of the terrestrial ecosystems, being insufficiently attracted by investigators. Based on high-throughput sequencing, the fungal community composition in some soil samples in tree holes of Robinia pseudoacacia grown with different plants in Guizhou urban green field was investigated. The plants growing in the tree hole soil include Solanum cathayanum, Ligustrum lucidum,Chenopodium serotinum, Hydrangea chinensis, Cichorium endivia and Achyranthes bidentata. The dominant genus of fungi is Metarhizium, Trechispora, an unknown fungus, an unclassified genus in Boliniaceae,Guehomyces and an unclassified genus in Nectriaceae, with relative abundance of 57.67%, 42.96%, 30.60%,86.45%, 33.15% and 12.26%, respectively. Some potential pathogens of insects and human beings and other unique fungi need further identification.
Tree hole is one special habitat of the terrestrial ecosystems, being insufficiently attracted by investigators. Based on high-throughput sequencing, the fungal community composition in some soil samples in tree holes of Robinia pseudoacacia grown with different plants in Guizhou urban green field was investigated. The plants growing in the tree hole soil include Solanum cathayanum, Ligustrum lucidum,Chenopodium serotinum, Hydrangea chinensis, Cichorium endivia and Achyranthes bidentata. The dominant genus of fungi is Metarhizium, Trechispora, an unknown fungus, an unclassified genus in Boliniaceae,Guehomyces and an unclassified genus in Nectriaceae, with relative abundance of 57.67%, 42.96%, 30.60%,86.45%, 33.15% and 12.26%, respectively. Some potential pathogens of insects and human beings and other unique fungi need further identification.
引文
Basset Y,2001.Invertebrates in the canopy of tropical rain forests how much do we really know?Plant Ecology,153(1-2):87-107
Che YS,2011.Discovery of new bioactive natural products from fungi of unique ecological niches.Journal of International Pharmaceutical Research,38(1):12-27(in Chinese)
Chen H,Hao HR,Xiong J,Qi XH,Zhang ZY,Lin WX,2007.Effects of successive cropping Rehmannia glutinosa on rhizosphere soil microbial flora and enzyme activities.Chinese Journal of Applied Ecology,18(12):2755-2759(in Chinese)
Chen WH,Zeng GP,Luo Y,Han YF,2017.Morphological traits and molecular analysis for Geomyces fujianensis sp.nov.from China.Mycosphere,8(1):38-43
Davies JL,Ngeleka M,Wobeser GA,2010.Systemic infection with Mortierella wolfii following abortion in a cow.The Canadian Veterinary Journal,51(12):1391-1393
Escudero N,Ferreira SR,Lopez-Moya F,Naramjo-Ortiz MA,Marin-Ortiz AI,Thornton CR,Lopez-Llorca LV,2016.Chitosan enhances parasitism of Meloidogyne javanica eggs by the nematophagous fungus Pochonia chlamydosporia.Fungal Biology,120(4):572-585
Fell JW,Scorzetti G,2004.Reassignment of the basidiomycetous yeasts Trichosporon pullulans to Guehomyces pullulans gen.nov.,comb.nov.and hyalodendron lignicola to Trichosporon lignicola comb.nov.International Journal of Systematic and Evolutionary Microbiology,54(3):995-998
Fouillaud M,Venkatachalam M,Llorente M,Magalon H,Cuet P,DufosséL,2017.Biodiversity of pigmented fungi isolated from marine environment in La Réunion island,Indian ocean:new resources for colored metabolites.Journal of Fungi,3(3):1895-1908
Gao J,Zhang YY,Wang K,Zhang J,Zhang G,Zhao J,2016.Identification of sunflower wilt pathogen and its biological characteristics.Chinese Journal of Oil Crop Science,38(2):214-222(in Chinese)
Geml J,Gravendeel B,van der Gaag KJ,Neilen M,Lammers Y,Raes N,Semenova TA,de Knijff P,Noordeloos ME,2014.The contribution of DNA metabarcoding to fungal conservation:diversity assessment,habitat partitioning and mapping red-listed fungi in protected coastal Salix repens communities in the Netherlands.PLoS One,9(6):e99852
Gloer JB,2007.15 applications of fungal ecology in the search for new bioactive natural products.Environmental and Microbial Relationships,4:257-283
Kerekes J,2014.Fungi up high:biodiversity and canopy soils.Mycena News,65:1-3(6)
Kurihara Y,Sukarno N,Ilyas M,Yuniarti E,Mangunwardoyo W,Saraswati R,Park JY,Inaba S,Widyastuti Y,Ando K,2008.Entomopathogenic fungi isolated from suspended-soil-inhabiting arthropods in East Kalimantan,Indonesia.Mycoscience,49(4):241-249
Liu CX,Song J,Wang XJ,Xiang WS,2016.Recruitment of defensive microbs and plant protection.Scientia Sinica(Vitae),46(5):491-497(in Chinese)
Man BY,2017.The diversity of fungal communities in Heshang cave(Hubei,China)and the potential role of fungal strains in calcium oxalate mineralization.PhDDissertation,China University of Geosciences,Wuhan.10-11(in Chinese)
Miao P,Wang HT,Li SJ,Kang YB,2014.First report of two pathogenic fungi on Tobacco in Henan Province.Chinese Tobacco Science,35(2):113-116(in Chinese)
Qin N,Li DF,Yang RF,2011.Next-generation sequencing technologies and the application in microbiology.Acta Microbiologica Sinica,51(4):445-457(in Chinese)
Reiter B,Pfeifer U,Schwab H,Sessitsch A,2002.Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp.atroseptica.Applied and Environmental Microbiology,68(5):2261-2268
Sang CP,Wan XH,Yu ZP,Wang MH,Lin Y,Huang ZQ,2017.Effects of litter and root exclusion on soil microbial community composition and function of four plantations in subtropical sandy coastal plain area,China.Chinese Journal of Applied Ecology,28(4):1184-1196(in Chinese)
Selvan PS,Jebanesan A,Reetha D,2016.Entomofaunal diversity of tree hole mosquitoes in western and eastern Ghats hill ranges of Tamilnadu,India.Acta Tropica,159:69-82
Shen X,Zhang ZY,Zheng H,Zou X,Han YF,Liang ZQ,2018.Influences of keratin-rich organic materials on fungal community composition in a hospital grassplot soil.Mycosystema,37(8):999-1005(in Chinese)
Stephenson SL,Landoit JC,2011.Dictyostelids from aerial“canopy soil”microhabitats.Fungal Ecology,4(3):191-195
Su XP,Hu GH,Wu J,Zou K,Guo ZY,Gong DC,2012.Isolation and antimicrobial activities of endophytic fungi in Solanum cathayanum.Lishizhen Medicine and Materia Medica Research,23(6):1337-1339(in Chinese)
Toledo AV,Virla E,Humber RA,Paradell SL,Lastra CCL,2006.First record of Clonostachys rosea(Ascomycota:Hypocreales)as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa(Hemiptera:Cicadellidae)in Argentina.Journal of Invertebrate Pathology,92(1):7-10
Wang JY,Xu JH,Wu LK,Wu HM,Zhu Q,Kong LF,Lin WX,2017.Analysis of physicochemical properties and microbial diversity in rhizosphere soil of Achyranthes bidentata under different cropping years.Acta Ecologica Sinica,37(17):5621-5629(in Chinese)
Winglee K,Howard AG,Sha W,Gharaubeh RZ,Liu J,Jin D,Fodorl AA,Gordon-Larsen P,2017.Recent urbanization in China is correlated with a westernized microbiome encoding increased virulence and antibiotic resistance genes.Microbiome,5(1):Doi:10.1186/s40168-017-0338-7
Xia J,2006.Studies on strains producing thermostable isoamylase from hot spring in Yunnan and the enzyme properties.Master Thesis,Kunming University of Science and Technology,Kunming.5(in Chinese)
Xu XL,Lin J,Yan RX,2018.Comparison of principle and application of the gene chip and high-throughput sequencing technologies.Chinese Journal of Biochemistry and Molecular Biology,34(11):1166-1174(in Chinese)
Zahouli JBZ,Koudou BG,Müller P,Malone D,Tano Y,Utzinger J,2017.Urbanization is a main driver for the larval ecology of Aedes mosquitoes in arbovirus-endemic settings in south-eastern C?te d’Ivoire.PLoS Neglected Tropical Diseases,11(7):e0005751
Zhalnina K,Louie KB,Hao Z,Mansoori N,da Rocha UN,Shi S,Cho H,Karaoz?U,LoquéD,Bowen BP,Firestone MK,Northen?TR,Brodie EL,2018.Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly.Nature Microbiology,3(4):470-480
Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2017.Community composition and diversity of culturable fungi from the soil suspended in tree holes of Robinia pseudoacacia.Mycosystema,36(5):625-632(in Chinese)
Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2018.Community composition and ecological functional structural analysis of the endophytic fungi in Robinia pseudoacacia.Mycosystema,37(2):256-263(in Chinese)
Zhou K,Guo WM,Xu YC,2004.Advances of research on allelopathic potential in compositae.Acta Ecologica Sinica,24(8):1780-1788(in Chinese)
Zhu LX,Zhang JE,Liu WG,2003.Review of studies on interactions between root exudates and rhizopheric microorganisms.Ecology and Environmental Sciences,12(1):102-105(in Chinese)
车永胜,2011.特殊生境真菌来源的新结构活性物质研究.国际药学研究杂志,38(1):12-27
陈慧,郝慧荣,熊君,齐晓辉,张重义,林文雄,2007.地黄连作对根际微生物区系及土壤酶活性的影响.应用生态学报,18(12):2755-2759
高婧,张园园,王凯,张键,张贵,赵君,2016.向日葵枯萎病菌的分离鉴定及其生物学特性.中国油料作物学报,38(2):214-222
刘重喜,宋佳,王相晶,向文胜,2016.植物招募防御微生物与植物保护.中国科学:生命科学,46(5):491-497
满百膺,2017.湖北清江和尚洞真菌群落多样性及真菌诱导草酸盐矿物的形成.中国地质大学博士论文,武汉.10-11
苗圃,王海涛,李淑君,康业兵,2014.两种病原真菌在河南危害烟草的首次报道.中国烟草科学,35(2):113-116
秦楠,栗东芳,杨瑞馥,2011.高通量测序技术及其在微生物学研究中的应用.微生物学报,51(4):445-457
桑昌鹏,万晓华,余再鹏,王民煌,林宇,黄志群,2017.凋落物和根系去除对滨海沙地土壤微生物群落组成和功能的影响.应用生态学报,28(4):1184-1196
沈鑫,张芝元,郑欢,邹晓,韩燕峰,梁宗琦,2018.富角蛋白有机物对医院绿地真菌群落组成的影响.菌物学报,37(8):999-1005
苏香萍,胡格华,吴军,邹坤,郭志勇,龚大春,2012.排风藤内生真菌的分离及抗菌活性的研究.时珍国医国药,23(6):1337-1339
王娟英,许佳慧,吴林坤,吴红淼,朱铨,孔露霏,林文雄,2017.不同连作年限怀牛膝根际土壤理化性质及微生物多样性.生态学报,37(17):5621-5629
夏静,2006.云南热泉栖热菌产耐热异淀粉酶的研究.昆明理工大学硕士论文,昆明.5
徐晓丽,林娟,鄢仁祥,2018.基因芯片与高通量测序技术的原理与应用的比较.中国生物化学与分子生物学报,34(11):1166-1174
郑欢,张芝元,韩燕峰,陈万浩,梁宗琦,2017.刺槐树洞悬土可培养真菌群落组成及其多样性分析.菌物学报,36(5):625-632
郑欢,张芝元,韩燕峰,陈万浩,梁宗琦,2018.刺槐内生真菌群落组成及其生态功能结构分析.菌物学报,37(2):256-263
周凯,郭维明,徐迎春,2004.菊科植物化感作用研究进展.生态学报,24(8):1780-1788
朱丽霞,章家恩,刘文高,2003.根系分泌物与根际微生物相互作用研究综述.生态环境学报,12(1):102-105
Che YS,2011.Discovery of new bioactive natural products from fungi of unique ecological niches.Journal of International Pharmaceutical Research,38(1):12-27(in Chinese)
Chen H,Hao HR,Xiong J,Qi XH,Zhang ZY,Lin WX,2007.Effects of successive cropping Rehmannia glutinosa on rhizosphere soil microbial flora and enzyme activities.Chinese Journal of Applied Ecology,18(12):2755-2759(in Chinese)
Chen WH,Zeng GP,Luo Y,Han YF,2017.Morphological traits and molecular analysis for Geomyces fujianensis sp.nov.from China.Mycosphere,8(1):38-43
Davies JL,Ngeleka M,Wobeser GA,2010.Systemic infection with Mortierella wolfii following abortion in a cow.The Canadian Veterinary Journal,51(12):1391-1393
Escudero N,Ferreira SR,Lopez-Moya F,Naramjo-Ortiz MA,Marin-Ortiz AI,Thornton CR,Lopez-Llorca LV,2016.Chitosan enhances parasitism of Meloidogyne javanica eggs by the nematophagous fungus Pochonia chlamydosporia.Fungal Biology,120(4):572-585
Fell JW,Scorzetti G,2004.Reassignment of the basidiomycetous yeasts Trichosporon pullulans to Guehomyces pullulans gen.nov.,comb.nov.and hyalodendron lignicola to Trichosporon lignicola comb.nov.International Journal of Systematic and Evolutionary Microbiology,54(3):995-998
Fouillaud M,Venkatachalam M,Llorente M,Magalon H,Cuet P,DufosséL,2017.Biodiversity of pigmented fungi isolated from marine environment in La Réunion island,Indian ocean:new resources for colored metabolites.Journal of Fungi,3(3):1895-1908
Gao J,Zhang YY,Wang K,Zhang J,Zhang G,Zhao J,2016.Identification of sunflower wilt pathogen and its biological characteristics.Chinese Journal of Oil Crop Science,38(2):214-222(in Chinese)
Geml J,Gravendeel B,van der Gaag KJ,Neilen M,Lammers Y,Raes N,Semenova TA,de Knijff P,Noordeloos ME,2014.The contribution of DNA metabarcoding to fungal conservation:diversity assessment,habitat partitioning and mapping red-listed fungi in protected coastal Salix repens communities in the Netherlands.PLoS One,9(6):e99852
Gloer JB,2007.15 applications of fungal ecology in the search for new bioactive natural products.Environmental and Microbial Relationships,4:257-283
Kerekes J,2014.Fungi up high:biodiversity and canopy soils.Mycena News,65:1-3(6)
Kurihara Y,Sukarno N,Ilyas M,Yuniarti E,Mangunwardoyo W,Saraswati R,Park JY,Inaba S,Widyastuti Y,Ando K,2008.Entomopathogenic fungi isolated from suspended-soil-inhabiting arthropods in East Kalimantan,Indonesia.Mycoscience,49(4):241-249
Liu CX,Song J,Wang XJ,Xiang WS,2016.Recruitment of defensive microbs and plant protection.Scientia Sinica(Vitae),46(5):491-497(in Chinese)
Man BY,2017.The diversity of fungal communities in Heshang cave(Hubei,China)and the potential role of fungal strains in calcium oxalate mineralization.PhDDissertation,China University of Geosciences,Wuhan.10-11(in Chinese)
Miao P,Wang HT,Li SJ,Kang YB,2014.First report of two pathogenic fungi on Tobacco in Henan Province.Chinese Tobacco Science,35(2):113-116(in Chinese)
Qin N,Li DF,Yang RF,2011.Next-generation sequencing technologies and the application in microbiology.Acta Microbiologica Sinica,51(4):445-457(in Chinese)
Reiter B,Pfeifer U,Schwab H,Sessitsch A,2002.Response of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp.atroseptica.Applied and Environmental Microbiology,68(5):2261-2268
Sang CP,Wan XH,Yu ZP,Wang MH,Lin Y,Huang ZQ,2017.Effects of litter and root exclusion on soil microbial community composition and function of four plantations in subtropical sandy coastal plain area,China.Chinese Journal of Applied Ecology,28(4):1184-1196(in Chinese)
Selvan PS,Jebanesan A,Reetha D,2016.Entomofaunal diversity of tree hole mosquitoes in western and eastern Ghats hill ranges of Tamilnadu,India.Acta Tropica,159:69-82
Shen X,Zhang ZY,Zheng H,Zou X,Han YF,Liang ZQ,2018.Influences of keratin-rich organic materials on fungal community composition in a hospital grassplot soil.Mycosystema,37(8):999-1005(in Chinese)
Stephenson SL,Landoit JC,2011.Dictyostelids from aerial“canopy soil”microhabitats.Fungal Ecology,4(3):191-195
Su XP,Hu GH,Wu J,Zou K,Guo ZY,Gong DC,2012.Isolation and antimicrobial activities of endophytic fungi in Solanum cathayanum.Lishizhen Medicine and Materia Medica Research,23(6):1337-1339(in Chinese)
Toledo AV,Virla E,Humber RA,Paradell SL,Lastra CCL,2006.First record of Clonostachys rosea(Ascomycota:Hypocreales)as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa(Hemiptera:Cicadellidae)in Argentina.Journal of Invertebrate Pathology,92(1):7-10
Wang JY,Xu JH,Wu LK,Wu HM,Zhu Q,Kong LF,Lin WX,2017.Analysis of physicochemical properties and microbial diversity in rhizosphere soil of Achyranthes bidentata under different cropping years.Acta Ecologica Sinica,37(17):5621-5629(in Chinese)
Winglee K,Howard AG,Sha W,Gharaubeh RZ,Liu J,Jin D,Fodorl AA,Gordon-Larsen P,2017.Recent urbanization in China is correlated with a westernized microbiome encoding increased virulence and antibiotic resistance genes.Microbiome,5(1):Doi:10.1186/s40168-017-0338-7
Xia J,2006.Studies on strains producing thermostable isoamylase from hot spring in Yunnan and the enzyme properties.Master Thesis,Kunming University of Science and Technology,Kunming.5(in Chinese)
Xu XL,Lin J,Yan RX,2018.Comparison of principle and application of the gene chip and high-throughput sequencing technologies.Chinese Journal of Biochemistry and Molecular Biology,34(11):1166-1174(in Chinese)
Zahouli JBZ,Koudou BG,Müller P,Malone D,Tano Y,Utzinger J,2017.Urbanization is a main driver for the larval ecology of Aedes mosquitoes in arbovirus-endemic settings in south-eastern C?te d’Ivoire.PLoS Neglected Tropical Diseases,11(7):e0005751
Zhalnina K,Louie KB,Hao Z,Mansoori N,da Rocha UN,Shi S,Cho H,Karaoz?U,LoquéD,Bowen BP,Firestone MK,Northen?TR,Brodie EL,2018.Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly.Nature Microbiology,3(4):470-480
Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2017.Community composition and diversity of culturable fungi from the soil suspended in tree holes of Robinia pseudoacacia.Mycosystema,36(5):625-632(in Chinese)
Zheng H,Zhang ZY,Han YF,Chen WH,Liang ZQ,2018.Community composition and ecological functional structural analysis of the endophytic fungi in Robinia pseudoacacia.Mycosystema,37(2):256-263(in Chinese)
Zhou K,Guo WM,Xu YC,2004.Advances of research on allelopathic potential in compositae.Acta Ecologica Sinica,24(8):1780-1788(in Chinese)
Zhu LX,Zhang JE,Liu WG,2003.Review of studies on interactions between root exudates and rhizopheric microorganisms.Ecology and Environmental Sciences,12(1):102-105(in Chinese)
车永胜,2011.特殊生境真菌来源的新结构活性物质研究.国际药学研究杂志,38(1):12-27
陈慧,郝慧荣,熊君,齐晓辉,张重义,林文雄,2007.地黄连作对根际微生物区系及土壤酶活性的影响.应用生态学报,18(12):2755-2759
高婧,张园园,王凯,张键,张贵,赵君,2016.向日葵枯萎病菌的分离鉴定及其生物学特性.中国油料作物学报,38(2):214-222
刘重喜,宋佳,王相晶,向文胜,2016.植物招募防御微生物与植物保护.中国科学:生命科学,46(5):491-497
满百膺,2017.湖北清江和尚洞真菌群落多样性及真菌诱导草酸盐矿物的形成.中国地质大学博士论文,武汉.10-11
苗圃,王海涛,李淑君,康业兵,2014.两种病原真菌在河南危害烟草的首次报道.中国烟草科学,35(2):113-116
秦楠,栗东芳,杨瑞馥,2011.高通量测序技术及其在微生物学研究中的应用.微生物学报,51(4):445-457
桑昌鹏,万晓华,余再鹏,王民煌,林宇,黄志群,2017.凋落物和根系去除对滨海沙地土壤微生物群落组成和功能的影响.应用生态学报,28(4):1184-1196
沈鑫,张芝元,郑欢,邹晓,韩燕峰,梁宗琦,2018.富角蛋白有机物对医院绿地真菌群落组成的影响.菌物学报,37(8):999-1005
苏香萍,胡格华,吴军,邹坤,郭志勇,龚大春,2012.排风藤内生真菌的分离及抗菌活性的研究.时珍国医国药,23(6):1337-1339
王娟英,许佳慧,吴林坤,吴红淼,朱铨,孔露霏,林文雄,2017.不同连作年限怀牛膝根际土壤理化性质及微生物多样性.生态学报,37(17):5621-5629
夏静,2006.云南热泉栖热菌产耐热异淀粉酶的研究.昆明理工大学硕士论文,昆明.5
徐晓丽,林娟,鄢仁祥,2018.基因芯片与高通量测序技术的原理与应用的比较.中国生物化学与分子生物学报,34(11):1166-1174
郑欢,张芝元,韩燕峰,陈万浩,梁宗琦,2017.刺槐树洞悬土可培养真菌群落组成及其多样性分析.菌物学报,36(5):625-632
郑欢,张芝元,韩燕峰,陈万浩,梁宗琦,2018.刺槐内生真菌群落组成及其生态功能结构分析.菌物学报,37(2):256-263
周凯,郭维明,徐迎春,2004.菊科植物化感作用研究进展.生态学报,24(8):1780-1788
朱丽霞,章家恩,刘文高,2003.根系分泌物与根际微生物相互作用研究综述.生态环境学报,12(1):102-105