燕窝多元素的分布及溯源信息研究
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摘要
收集不同来源的燕窝48盏,采用电感耦合等离子质谱(ICP-MS)对不同来源燕窝中的27种元素进行了分析,发现燕窝中含有5种呈Na>Ca>Mg>K>P分布的常量元素、生物体必需微量元素(Fe、Cu、Zn、Mn、Mo、Co、Se、Cr)、3种轻稀土(La、Ce、Nd)和2种重稀土(Tb和Lu)。燕窝的27种元素中,除Pt外均呈偏态分布且变异系数(COV)较大。基于SPSS 20.0的t-检验、MannWhitney U检验、个案排秩(Tukey正态得分)及双因素方差分析技术,从燕窝的产地、采收方式及产地×采收方式交互作用等方面对变异原因进行分析,筛选出采收方式溯源特征显著的元素B、Na、P、Ca、Mn、Cu、Sr、Mo、Ba、Nd和产地溯源特征显著的元素Mg、Al、Pt。研究证实了基于多元素分析和化学计量学模型的燕窝溯源研究的可行性。
Forth-eight cup-shaped edible bird's nest(EBN)harvesting from natural cave and man-made house were collected from Malaysia and Indonesia to study the distribution characteristics and traceability of 27 elements in EBN.The inductively coupled plasma mass spectrometry(ICP-MS)was applied for the detection of multi-elements in EBN,and five macro-elements which distributed according to the rule of Na>Ca> Mg>K>P were found in EBN.Besides,in EBN,some essential trace elements(Fe,Cu,Zn,Mn,Mo,Co,Se,Cr),3 light rare earth elements(LREE)and 2 heavy rare earth elements(HREE)also be detected.All of 27 elements in EBN is skewed distribution with bigger coefficient of variation(COV)except for Pt.The t-test,MannWhitney U,Rank Cases based on the Tukey formula and Double factor variance analysis was used to analyze the influence of geographical origin,harvesting method and their interactions on the distribution of multi-elements in EBN.B,Na,P,Ca,Mn,Cu,Sr,Mo,Ba and Nd were significantly relative with harvesting method of EBN,and Mg,Al and Pt were for geographical origin of EBN.This article not only verified the feasibility to trace the provenance of cup-shaped EBN,but also provided a reference for traceability research, Nutritional function development and quality grade assessment of EBN.
Forth-eight cup-shaped edible bird's nest(EBN)harvesting from natural cave and man-made house were collected from Malaysia and Indonesia to study the distribution characteristics and traceability of 27 elements in EBN.The inductively coupled plasma mass spectrometry(ICP-MS)was applied for the detection of multi-elements in EBN,and five macro-elements which distributed according to the rule of Na>Ca> Mg>K>P were found in EBN.Besides,in EBN,some essential trace elements(Fe,Cu,Zn,Mn,Mo,Co,Se,Cr),3 light rare earth elements(LREE)and 2 heavy rare earth elements(HREE)also be detected.All of 27 elements in EBN is skewed distribution with bigger coefficient of variation(COV)except for Pt.The t-test,MannWhitney U,Rank Cases based on the Tukey formula and Double factor variance analysis was used to analyze the influence of geographical origin,harvesting method and their interactions on the distribution of multi-elements in EBN.B,Na,P,Ca,Mn,Cu,Sr,Mo,Ba and Nd were significantly relative with harvesting method of EBN,and Mg,Al and Pt were for geographical origin of EBN.This article not only verified the feasibility to trace the provenance of cup-shaped EBN,but also provided a reference for traceability research, Nutritional function development and quality grade assessment of EBN.
引文
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[21]JR N M,AZMAN O,WAN N W.Preliminary study of the nutritional content of malaysian edible bird’s nest[J].Malaysian Journal of Nutrition,2010,16(3):389-396.
[22]简叶叶,李庆旺,黄灿灿,等.干燕窝与炖煮燕窝的营养成分分析与比较[J].福建轻纺,2016(3):32-38.
[23]乌日罕,陈颖,吴亚君,等.燕窝真伪鉴别方法及国内外研究进展[J].检验检疫学刊,2007,17(4):60-62.
[24]张在海,王淀佐,邱冠周,等.细菌浸矿的细菌学原理[J].湿法冶金,2000,19(3):16-21.
[25]KHAN A M,YUSOFF I,BAKAR N K A,et al.Accumulation,uptake and bioavailability of rare earth elements(REEs)in soil grown plants from ex-mining area in Perak,Malaysia[J].Applied Ecology&Environmental Research,2017,15(3):117-133.
[26]LOURIE S A,TOMPKINS D M.The diets of Malaysian swiftlets[J].Ibis,2010,142(4):596-602.
[2]林洁茹.燕窝DNA基原鉴定及抗病毒作用研究[D].广州:广州中医药大学,2010.
[3]王凤云,韩亮,赖小平.32种不同产地与品种商品燕窝的DNA基源鉴定[J].世界科学技术:中医药现代化,2015(9):1 876-1 882.
[4]HOBBS J J.Problems in the harvest of edible birds’nests in Sarawak and Sabah,Malaysian Borneo[J].Biodiversity&Conservation,2004,13(12):2 209-2 226.
[5]张北山.燕窝中三种含硫活性成分的提取和检测[D].济南:山东师范大学,2013:19-24.
[6]KONG Y C,KEUNG W M,T T Y,et al.Evidence that epidermal growth factor is present in swiftlet’s(Collocalia)nest[J].Comparative Biochemistry and Physiology B,Comparative Biochemistry,1987,2(87):221-226.
[7]NURFATIN M H,SYARMILA I K E,NUR’ALIAH D,et al.Effect of enzymatic hydrolysis on Angiotensin converting enzyme(ACE)inhibitory activity in swiftlet saliva[J].International Food Research Journal,2016,23(1):141-146.
[8]GUO C T,TAKAHASHI T,BUKAWA W,et al.Edible bird’s nest extract inhibits influenza virus infection[J].Antiviral Research,2006,70(3):140-146.
[9]CHAN G K L,WONG Z C F,LAM K Y C,et al.Edible Bird’s Nest,an Asian Health Food Supplement,Possesses Skin Lightening Activities:Identification of N-Acetylneuraminic Acid as Active Ingredient[J].Journal of Cosmetics,Dermatological Sciences and Applications,2015,5(4):262-274.
[10]CHUA K H,LEE T H,NAGANDRAN K,et al.Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee:in vitro study[J].BMC Complementary and Alternative Medicine,2013,13(1):19-27.
[11]孙利,蒋萍萍,王欣,等.进口燕窝溯源系统的构建和应用[J].食品安全质量检测学报,2017,8(5):1 894-1 898.
[12]曹妍,徐杰,高焱,等.白燕与血燕的营养成分分析和比较[J].食品工业科技,2011(10):414-417.
[13]QUEK M C,CHIN N L,YUSOF Y A,et al.Characterization of edible bird’s nest of different production,species and geographical origins using nutritional composition,physicochemical properties and antioxidant activities[J].Food Research International,2018,109:35-43.
[14]MARCONE M F.Characterization of the edible bird’s nest the“Caviar of the East”[J].Food Research International,2005,38(10):1 125-1 134.
[15]SAENGKRAJANG W,MATAN N,MATAN N.Nutritional composition of the farmed edible bird’s nest(Collocalia fuciphaga)in Thailand[J].Journal of Food Composition and Analysis,2013,31(1):41-45.
[16]郭秋兰,孙艺,宋美英.电感耦合等离子体质谱法同时测定燕窝中14种元素含量[J].粮食流通技术,2016,5(9):103-106.
[17]由艳燕,李兆杰,徐杰,等.6种市售燕窝营养成分分析[J].营养学报,2012,34(4):400-402.
[18]赵斌,邓仙梅,王羚郦,等.ICP-MS测定东南亚进口燕窝无机元素的主成分分析和聚类分析[J].中药材,2015,38(4):697-700.
[19]SEOW E K,IBRAHIM B,MUHAMMAD S A,et al.Discrimination between cave and house-farmed edible bird’s nest based on major mineral profiles[J].Pertanika Journal of Tropical Agricultural Science,2016,39(2):181-195.
[20]ZHANG Xiao-wen,WEI Shuai,SUN Qian-qian,et al.Source identification and spatial distribution of arsenic and heavy metals in agricultural soil around Hunan industrial estate by positive matrix factorization model,principle components analysis and geo statistical analysis[J].Ecotoxicology and Environmental Safety,2018,159:354-362.
[21]JR N M,AZMAN O,WAN N W.Preliminary study of the nutritional content of malaysian edible bird’s nest[J].Malaysian Journal of Nutrition,2010,16(3):389-396.
[22]简叶叶,李庆旺,黄灿灿,等.干燕窝与炖煮燕窝的营养成分分析与比较[J].福建轻纺,2016(3):32-38.
[23]乌日罕,陈颖,吴亚君,等.燕窝真伪鉴别方法及国内外研究进展[J].检验检疫学刊,2007,17(4):60-62.
[24]张在海,王淀佐,邱冠周,等.细菌浸矿的细菌学原理[J].湿法冶金,2000,19(3):16-21.
[25]KHAN A M,YUSOFF I,BAKAR N K A,et al.Accumulation,uptake and bioavailability of rare earth elements(REEs)in soil grown plants from ex-mining area in Perak,Malaysia[J].Applied Ecology&Environmental Research,2017,15(3):117-133.
[26]LOURIE S A,TOMPKINS D M.The diets of Malaysian swiftlets[J].Ibis,2010,142(4):596-602.
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