西山焦枣多糖的超声辅助法提取工艺和抗氧化研究
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摘要
为了进一步开发我国传统食品的功能成分,以池州西山焦枣为研究对象,在超声条件下探讨了焦枣多糖的提取条件,采用单因素和三水平四因素正交表L9(34)设计实验,优化了提取条件,经分析计算确定枣多糖的最佳工艺提取条件:温度90℃,超声功率90w,浸提时间25min,料液比1:30,提取2次,枣多糖的产率可达到5.58mg/L;经红外光谱图分析,枣多糖为β吡喃葡聚糖。
In order to explore the functional component of traditional Chinese food, the polysaccharide is extracted by ultrasonic from Xishan dates.The influence factors of temperature,extraction time,ratio of material to solution,the power of ultrasonic on the extraction ratio are investigated by means of orthogonal experiment of the four factors and three levels orthogonal table L9(34), The results shows that the optimum conditions with the extraction ratio reaches 5.58 mg/L with 2 times extraction, the temperature is 90 ℃,the time is 25 min,the ratio of material to solution is 1:30,the power of ultrasonic is 90 W. The polysaccharide is β-pyran glucan with analysis of infrared spectrogram.
In order to explore the functional component of traditional Chinese food, the polysaccharide is extracted by ultrasonic from Xishan dates.The influence factors of temperature,extraction time,ratio of material to solution,the power of ultrasonic on the extraction ratio are investigated by means of orthogonal experiment of the four factors and three levels orthogonal table L9(34), The results shows that the optimum conditions with the extraction ratio reaches 5.58 mg/L with 2 times extraction, the temperature is 90 ℃,the time is 25 min,the ratio of material to solution is 1:30,the power of ultrasonic is 90 W. The polysaccharide is β-pyran glucan with analysis of infrared spectrogram.
引文
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[11]刘芳,陈贵堂,胡秋辉,等.金针菇锌多糖分离纯化及其结构特征[J].食品科学,2014,35(5):1-7.
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[14]孙汉巨,潘丽军,靳锁.油菜花多糖的提取及纯化研究[J].食品科学,2006,27(12):432-436.
[15]高蒙蒙,赵峡,王清池,等.太平洋牡蛎多糖的提取、分离和结构分析[J].中国海洋药物,2014,33(4):8-14.
[16]李宏燕,樊君.大枣多糖的提取分离及纯化研究[J].宁夏工程技术,2006,5(2):145-147.
[17]潘剑用,汪志平,党江波,等.夏桑叶的体外抗氧化活性及其主要功能成分研究[J].核农学报,2011,25(4):754-759,778.
[18]Valent?o P,Fernandes E,Carvalho F,Andrade P B,Seabra R M,Bastos M L. Antioxidative Properties of Cardoon(Cynara cardunculus L.)Infusion Against Superoxide Radical,Hydroxyl Radical, and Hypochlorous Acid[J].Journal of Agricultural and Food Chemistry,2002,50(17):4989-4993.
[19]Parejo I,Viladomat F,Bastida J,Rosas-Romero A,Saavedra G,Murcia M A,Jiménez A M,Codina C. Investigation of Bolivian plant extracts for their radical scavenging activity and antioxidant activity[J].Life Sciences,2003,73(13):1667-1681.
[2]Zhao Z,Liu M,Tu P. Characterization of water soluble polysaccharides from organs of Chinese Jujube(Ziziphus jujuba Mill. cv.Dongzao)[J].European Food Research and Technology,2008,226(5):985-989.
[3]Jiang J-G,Huang X-J,Chen J,Lin Q-S. Comparison of the sedative and hypnotic effects of flavonoids,saponins,and polysaccharides extracted from Semen Ziziphus jujube[J].Natural Product Research,2007,21(4):310-320.
[4]Shengjing L X L. Extraction of Jujube Ploysaccharide and Its Effect of Cleaning Oxygen Radical[J]. GUANGXI SCIENCES,2000(1):016.
[5]林勤保,高大维,于淑娟,等.大枣多糖的分离和纯化[J].食品工业科技,1998(4):22-23.
[6]张秀梅,骆党委,朱祝英,等.3种热带果树叶子甲醇粗提物的抗氧化活性比较[J].核农学报,2015,29(1):95-100.
[7]蒲彪,岳金玫,陈安均,等.响应面法优化块菌多糖的超声波辅助提取工艺[J].核农学报,2013,27(7):996-1002.
[8]杨娜,王鸿飞,宋佳敏,等.超声波辅助提取裂褶菌多糖及分离纯化的研究[J].核农学报,2014,28(11):2015-2024.
[9]李满秀,刘美云.山楂中熊果酸的超声提取及抗氧化性研究[J].食品工业科技,2010,31(4):280-282.
[10]鲁吉珂,古国峰,汪瑞,等.蝉花多糖提取工艺优化研究[J].食品工业科技,2013,34(22):196-199.
[11]刘芳,陈贵堂,胡秋辉,等.金针菇锌多糖分离纯化及其结构特征[J].食品科学,2014,35(5):1-7.
[12]Schempp H,Weiser D,Elstner E F. Biochemical model reactions indicative of inflammatory processes[J].Arzneimittelforschung,2000,50(4):362-372.
[13]Janisch K,Hippeli S,Dornisch K,Kern S,Elstner E F. Determination of the antioxidative potential of human plasma after supplementation with pycnogenol and whey[J].Food research international,2002,35(2):257-266.
[14]孙汉巨,潘丽军,靳锁.油菜花多糖的提取及纯化研究[J].食品科学,2006,27(12):432-436.
[15]高蒙蒙,赵峡,王清池,等.太平洋牡蛎多糖的提取、分离和结构分析[J].中国海洋药物,2014,33(4):8-14.
[16]李宏燕,樊君.大枣多糖的提取分离及纯化研究[J].宁夏工程技术,2006,5(2):145-147.
[17]潘剑用,汪志平,党江波,等.夏桑叶的体外抗氧化活性及其主要功能成分研究[J].核农学报,2011,25(4):754-759,778.
[18]Valent?o P,Fernandes E,Carvalho F,Andrade P B,Seabra R M,Bastos M L. Antioxidative Properties of Cardoon(Cynara cardunculus L.)Infusion Against Superoxide Radical,Hydroxyl Radical, and Hypochlorous Acid[J].Journal of Agricultural and Food Chemistry,2002,50(17):4989-4993.
[19]Parejo I,Viladomat F,Bastida J,Rosas-Romero A,Saavedra G,Murcia M A,Jiménez A M,Codina C. Investigation of Bolivian plant extracts for their radical scavenging activity and antioxidant activity[J].Life Sciences,2003,73(13):1667-1681.
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