眼点淀粉卵涡鞭虫包囊阶段生活史的观察及防治
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摘要
自发病的大黄鱼鳃上取得眼点淀粉卵涡鞭虫滋养体,置于凹玻片上连续观察其分裂过程。淀粉卵涡虫包囊的直径为61.3~112.7μm,平均(85.3±16.0)μm。以二分裂的方式分裂,每完成一次分裂细胞数量增加1倍。水温27~29℃时,大多数滋养体经8~12h后进入二细胞期,此后4~10h完成一次分裂。所观察的包囊中,最多的能完成8次分裂,最少的完成2次分裂。不同分裂次数的包囊均孵出涡孢子,滋养体脱落至涡孢子孵出需48~66h。硫酸铜硫酸亚铁合剂可以对大黄鱼苗鳃丝上的眼点淀粉卵涡鞭虫滋养体起到明显的驱除作用。
The theronts of parasite Amyloodinium ocellatum were collected from infected large yellow croaker,Larimichthys crocea,and placed on the concave glass for observation of the continuously splitting process.It was found that the theronts had diameter of 61.3-112.7μm,and reproduction with two division ways,one time increase in cell quantity by each completed cell division.Most of the theronts developed into two cell phase in 8-12 h,then completed a division every 4-10 hat water temperature of 27-29℃,with the maximum 8times and the minimum 2times.The spores were hatched from all the theronts with different division frequency in 48-66 h.The obvious removal of the theronts were observed in the gill filaments of the large yellow croaker exposed to mixture of copper sulfate and ferrous sulfate.
The theronts of parasite Amyloodinium ocellatum were collected from infected large yellow croaker,Larimichthys crocea,and placed on the concave glass for observation of the continuously splitting process.It was found that the theronts had diameter of 61.3-112.7μm,and reproduction with two division ways,one time increase in cell quantity by each completed cell division.Most of the theronts developed into two cell phase in 8-12 h,then completed a division every 4-10 hat water temperature of 27-29℃,with the maximum 8times and the minimum 2times.The spores were hatched from all the theronts with different division frequency in 48-66 h.The obvious removal of the theronts were observed in the gill filaments of the large yellow croaker exposed to mixture of copper sulfate and ferrous sulfate.
引文
[1]许斌福,林能锋,陈振海,等.淀粉卵甲藻的危害与防治[J].福建畜牧兽医,1999,21(6):28.
[2]何祥楷.大黄鱼淀粉卵涡鞭虫病的诊断与防治方法[J].福建水产,2006,35(6):475-478.
[3]徐绍刚,朱华,田照辉.漠斑牙鲆淀粉卵甲藻病的防治[J].科学养鱼,2008,24(7):48-49.
[4]杜庆红,陈栩,朱长寿,等.海马卵甲藻病的防治[J].海洋科学,2005,29(11):4-7.
[5]徐润林,江静波,陈毕生.刺激隐核虫生活史的光镜观查[J].海洋科学,1992,16(3):42-44.
[6]刘振勇,谢友佺.刺激隐核虫生活史的观查[J].福建水产,2010,26(1):46-48.
[7]徐润林.刺激隐核虫研究的过去与现在[J].南海研究与开发,1992(3):8-13.
[8]Dickerson H W.Ichthyophthirius multifiliis and Cryptocaryon irritans(Phylum Ciliophora)[J].Fish Diseases and Disorders,2006(1):116-153.
[9]Dan X M,Li A X,Lin X T,et al.A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus[J].Aquaculture,2006,258(1/4):127-133.
[10]周旻曦.大黄鱼刺激隐核虫病病原生物学特性的研究[D].宁波:宁波大学,2012:1-6.
[11]但学明.刺激隐核虫的传代、保存及刺激隐核虫病的防治研究[D].广州:暨南大学,2006:1-4.
[12]王大鹏,何安尤,谢达详,等.龙胆石斑刺激隐核虫病防治技术研究[J].水产科技情报,2013,40(1):22-26.
[13]邬阳,牛素芳,金媛,等.闽东海域刺激隐核虫的生活史观查及乙酸杀虫效果[J].厦门大学学报,2013,52(2):281-288.
[14]陈丽达,张其中.杀灭离体小瓜虫的药物试验[J].淡水渔业,2005,35(1):45-46.
[15]陈生生,何伟林.乙酸治疗小瓜虫病的初步试验[J].动物学杂志,1980,15(1):37-38.
[16]邹峰,苏永全,谭映雪,等.海水鱼类寄生虫刺激隐核虫趋化性研究[J].海洋与湖沼,2013,44(4):1003-1007.
[17]王印庚,刘志伟,林春媛,等.养殖大菱鲆隐核虫病及其治疗[J].水产学报,2011,35(7):1105-1111.
[18]王忠华,张辰仓,王印庚,等.网箱养殖大黄鱼刺激隐核虫病及其防治[J].水产科技情报,2012,39(6):293-298.
[2]何祥楷.大黄鱼淀粉卵涡鞭虫病的诊断与防治方法[J].福建水产,2006,35(6):475-478.
[3]徐绍刚,朱华,田照辉.漠斑牙鲆淀粉卵甲藻病的防治[J].科学养鱼,2008,24(7):48-49.
[4]杜庆红,陈栩,朱长寿,等.海马卵甲藻病的防治[J].海洋科学,2005,29(11):4-7.
[5]徐润林,江静波,陈毕生.刺激隐核虫生活史的光镜观查[J].海洋科学,1992,16(3):42-44.
[6]刘振勇,谢友佺.刺激隐核虫生活史的观查[J].福建水产,2010,26(1):46-48.
[7]徐润林.刺激隐核虫研究的过去与现在[J].南海研究与开发,1992(3):8-13.
[8]Dickerson H W.Ichthyophthirius multifiliis and Cryptocaryon irritans(Phylum Ciliophora)[J].Fish Diseases and Disorders,2006(1):116-153.
[9]Dan X M,Li A X,Lin X T,et al.A standardized method to propagate Cryptocaryon irritans on a susceptible host pompano Trachinotus ovatus[J].Aquaculture,2006,258(1/4):127-133.
[10]周旻曦.大黄鱼刺激隐核虫病病原生物学特性的研究[D].宁波:宁波大学,2012:1-6.
[11]但学明.刺激隐核虫的传代、保存及刺激隐核虫病的防治研究[D].广州:暨南大学,2006:1-4.
[12]王大鹏,何安尤,谢达详,等.龙胆石斑刺激隐核虫病防治技术研究[J].水产科技情报,2013,40(1):22-26.
[13]邬阳,牛素芳,金媛,等.闽东海域刺激隐核虫的生活史观查及乙酸杀虫效果[J].厦门大学学报,2013,52(2):281-288.
[14]陈丽达,张其中.杀灭离体小瓜虫的药物试验[J].淡水渔业,2005,35(1):45-46.
[15]陈生生,何伟林.乙酸治疗小瓜虫病的初步试验[J].动物学杂志,1980,15(1):37-38.
[16]邹峰,苏永全,谭映雪,等.海水鱼类寄生虫刺激隐核虫趋化性研究[J].海洋与湖沼,2013,44(4):1003-1007.
[17]王印庚,刘志伟,林春媛,等.养殖大菱鲆隐核虫病及其治疗[J].水产学报,2011,35(7):1105-1111.
[18]王忠华,张辰仓,王印庚,等.网箱养殖大黄鱼刺激隐核虫病及其防治[J].水产科技情报,2012,39(6):293-298.