HPLC同时测定心可宁胶囊中羟基红花黄色素A和丹酚酸B的含量
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摘要
目的建立同时测定心可宁胶囊中羟基红花黄色素A和丹酚酸B含量的方法。方法采用高效液相色谱法(HPLC),色谱柱为Agilent Zorbax Extend C_(18)柱(250 mm×4.6 mm,5μm),柱温30℃,流动相为甲醇-0.5%磷酸(35:65),流速1.0 ml/min,检测波长403 nm(羟基红花黄色素A)、286 nm(丹酚酸B),进样量10μl。结果羟基红花黄色素A在0.1184～0.8880μg的范围内呈良好的线性关系(r=0.9994),丹酚酸B在0.8390～6.2925μg的范围内呈良好的线性关系(r=0.9998);羟基红花黄色素A平均加样回收率为99.17%(n=6),RSD为0.87%,丹酚酸B平均加样回收率为99.41%,RSD为0.87%。结论该方法操作简便、准确、重复性好,可用于心可宁胶囊的质量控制。
Objective To establish a method for the simultancous determination of hydroxysafflor yellow A and salvianolic aicd B in Xinkening Capsules by HPLC. Methods HPLC was performed on an Agilent Zorbax Extend C_(18)(250 mm×4.6 mm, 5 μm) with the column temperature of 30 ℃. The moblic phase was methanol-0.5 % phosphoric acid(35:65) at a ?ow rate of 1.0 ml/min, the detection wavelength was 403 nm(for hydroxysaf?or yellow A) and 286 nm(for salvianolic aicd B) and the injection volume was 10 μl. Results Hydroxysaf?or yellow A presented a good linearity in the concentration range of 0.1184-0.8880 μg(r=0.9994). Salvianolic aicd B presented a good linearity in the concentration range of 0.8390-6.2925 μg(r=0.9998). The average rate recovery of hydroxysaf?or yellow A was99.17 %(n=6), with RSD of 0.87 %. The average rate recovery aof salvianolic aicd B was 99.41 %, with RSD of 0.87 %. Conclusion This method is simple, accurate and reproducible, thus can be used for quality control of Xinkening Capsules.
Objective To establish a method for the simultancous determination of hydroxysafflor yellow A and salvianolic aicd B in Xinkening Capsules by HPLC. Methods HPLC was performed on an Agilent Zorbax Extend C_(18)(250 mm×4.6 mm, 5 μm) with the column temperature of 30 ℃. The moblic phase was methanol-0.5 % phosphoric acid(35:65) at a ?ow rate of 1.0 ml/min, the detection wavelength was 403 nm(for hydroxysaf?or yellow A) and 286 nm(for salvianolic aicd B) and the injection volume was 10 μl. Results Hydroxysaf?or yellow A presented a good linearity in the concentration range of 0.1184-0.8880 μg(r=0.9994). Salvianolic aicd B presented a good linearity in the concentration range of 0.8390-6.2925 μg(r=0.9998). The average rate recovery of hydroxysaf?or yellow A was99.17 %(n=6), with RSD of 0.87 %. The average rate recovery aof salvianolic aicd B was 99.41 %, with RSD of 0.87 %. Conclusion This method is simple, accurate and reproducible, thus can be used for quality control of Xinkening Capsules.
引文
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[7]邵先周,徐志成,施务务.HPLC法测定心可宁胶囊羟基红花黄色素A的含量[J].宿州学院学报, 2011, 26(2):37-39.
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[9]国家药典委员会.中国药典2015年版一部[S].北京:中国医药科技出版社, 2015:151.
[2]田静,李奇,闻琍毓.心可宁胶囊中非法添加物金橙Ⅱ和樟脑的检测研究[J].天津药学, 2015, 27(1):1-5.
[3]卫生部药品标准中药成分制剂第九册心可宁胶囊[S].1994:48-49.
[4]董凤彩.丹参成分及其药理作用[J].中国药物经济学, 2015,(5):99-100.
[5]易善勇,官丽莉,杨晶,等.红花药理作用及其开发与应用研究进展[J].北方园艺, 2015,(5):191-195.
[6]朱莉红.红花药理分析及临床应用分析[J].中国现代药物应用,2016, 10(16):286-287.
[7]邵先周,徐志成,施务务.HPLC法测定心可宁胶囊羟基红花黄色素A的含量[J].宿州学院学报, 2011, 26(2):37-39.
[8]钟萌,韩杰.HPLC测定心可宁胶囊中丹酚酸B的含量[J].中国药师, 2009, 12(12):1759-1761.
[9]国家药典委员会.中国药典2015年版一部[S].北京:中国医药科技出版社, 2015:151.