蒸汽渗透技术用于细辛挥发油含油水体分离的可行性研究
|
摘要
目的验证蒸汽渗透技术分离细辛挥发油含油水体的可行性,进而将蒸汽渗透技术应用于更多中药挥发油含油水体的分离。方法以聚二甲基硅氧烷/聚偏氟乙烯(PDMS/PVDF)复合平板膜和PVDF平板膜为膜材料,采用蒸汽渗透技术分离细辛挥发油含油水体,计算2种膜的透油率,同时通过气相色谱-质谱(GC-MS)联用技术分析比较过膜前后挥发油成分种类和含量的变化。结果以PVDF膜为膜材料进行蒸汽渗透实验时,其挥发油的透油率明显高于PDMS/PVDF膜,GC-MS定性分析结果显示,2种膜的渗透液中挥发油的成分与传统水蒸气蒸馏法得到的挥发油中的成分基本一致,以双内标法对其中的α-蒎烯、β-蒎烯、3,5-二甲氧基甲苯和甲基丁香酚4种成分进行含量测定,结果发现PVDF膜渗透液中各成分的含量明显高于PDMS/PVDF膜渗透液。结论蒸汽渗透膜技术分离细辛挥发油含油水体是可行的,且与PDMS/PVDF膜相比,PVDF膜更适用于分离细辛挥发油含油水体。
Objective To verify the feasibility of vapor-permeable membrane technology for the separation of water bodies containing essential oil of Asari Radix et Rhizoma(ARR) essential oil, and then to apply vapor permeate technology to the separation of more essential oils of traditional Chinese medicine. Methods The polydimethylsiloxane/polyvinylidene fluoride(PDMS/PVDF) composite flat membrane and polyvinylidene fluoride(PVDF) flat membrane were collected as the membrane material. The oil-bearing water body of ARR volatile oil was separated by vapor permeate technology, and the oil penetration rate of two kinds of membranes was calculated. At the same time, the changes of the composition and content of the essential oil before and after the membrane were analyzed by gas chromatography-mass spectrometry(GC-MS). Results The results showed that the essential oil penetration rate was significantly higher than that of PDMS/PVDF membrane when PVDF membrane was used as membrane material. GC-MS qualitative analysis results showed that the composition of the essential oil in the penetrants of the two membranes was basically the same as that of the essential oil obtained by the traditional steam distillation method. The content of α-pinene, β-pinene, 3,5-dimethoxytoluene, and methyl eugenol were determined by double internal standard method. The results showed that the content of each component in the PVDF membrane permeation was significantly higher than that of the PDMS/PVDF membrane permeation solution. Conclusion It is feasible to separate the oil containing water from the essential oil of ARR by vapor permeation membrane technology. Compared with the PDMS/PVDF membrane, the PVDF membrane is more suitable for separating the oil containing water of the essential oil of ARR.
Objective To verify the feasibility of vapor-permeable membrane technology for the separation of water bodies containing essential oil of Asari Radix et Rhizoma(ARR) essential oil, and then to apply vapor permeate technology to the separation of more essential oils of traditional Chinese medicine. Methods The polydimethylsiloxane/polyvinylidene fluoride(PDMS/PVDF) composite flat membrane and polyvinylidene fluoride(PVDF) flat membrane were collected as the membrane material. The oil-bearing water body of ARR volatile oil was separated by vapor permeate technology, and the oil penetration rate of two kinds of membranes was calculated. At the same time, the changes of the composition and content of the essential oil before and after the membrane were analyzed by gas chromatography-mass spectrometry(GC-MS). Results The results showed that the essential oil penetration rate was significantly higher than that of PDMS/PVDF membrane when PVDF membrane was used as membrane material. GC-MS qualitative analysis results showed that the composition of the essential oil in the penetrants of the two membranes was basically the same as that of the essential oil obtained by the traditional steam distillation method. The content of α-pinene, β-pinene, 3,5-dimethoxytoluene, and methyl eugenol were determined by double internal standard method. The results showed that the content of each component in the PVDF membrane permeation was significantly higher than that of the PDMS/PVDF membrane permeation solution. Conclusion It is feasible to separate the oil containing water from the essential oil of ARR by vapor permeation membrane technology. Compared with the PDMS/PVDF membrane, the PVDF membrane is more suitable for separating the oil containing water of the essential oil of ARR.
引文
[1]中国药典[S].一部.2015.
[2]覃梦瑶,阮文懿,翟苑好,等.三伏贴中芥子碱及细辛挥发油促进HaCaT细胞摄取延胡索乙素的作用及其机制研究[J].中草药,2018,49(2):400-405.
[3]刘海燕,范婧,高微微,等.细辛挥发油对植物病原真菌的抑制作用研究[J].中草药,2007,38(12):1878-1881.
[4]Wang B,Yuan D.Comparative study of chemical composition,antinociceptive effect and acute toxicity of the essential oils of three Asarum drugs[J].J Chin Pharm Sci,2014,23(7):480-489.
[5]费可,胡瑜瑕,周璐,等.细辛临床应用与药理作用研究进展[J].上海中医药大学学报,2010,24(6):87-90.
[6]唐锋,梁少瑜,田元新,等.细辛挥发油抗过敏性鼻炎有效成分及靶点预测的研究[J].中国实验方剂学杂志,2015,21(24):126-131.
[7]刘东吉,刘春生.不同产地栽培辽细辛的挥发油研究[J].中国实验方剂学杂志,2010,16(9):79-82.
[8]杜成智,陈玉萍,覃洁萍,等.不同产地细辛挥发油的GC-MS分析[J].中国实验方剂学杂志,2011,17(7):57-59.
[9]梁学清,李丹丹.细辛药理作用研究进展[J].河南科技大学学报:医学版,2011,29(4):318-320.
[10]潘磊,郑柳,牛卉,等.细辛挥发油成分提取与甲基丁香酚含量测定的研究进展[J].时珍国医国药,2015,26(4):967-970.
[11]Senatore F,Arnold N A,Bruno M.Essential components of Centaurea eryngiodes Lam.and Centaurea iberica Trev.var.hermonis Bois.Lam.,two Asteraceae growing wild in Lebanon[J].Nat Prod Res,2005,19(8):749-754.
[12]陆钊,高凯,潘淑霞,等.加速溶剂萃取/气相色谱-质谱法分析朝鲜淫羊藿挥发油成分[J].哈尔滨工业大学学报,2011,43(8):145-148.
[13]Kim M R,El-Aty A M,Choi J H,et al.Identification of essential components in Angelica species using supercritical-CO2 fluid extraction and solid phase microextraction coupled to gas chromatography-mass spectrometry[J].Biomed Chromatogr,2006,20(11):1267-1273.
[14]何颖.中药挥发油提取方法分析[J].天津药学,2015,27(1):47-50.
[15]伍振峰,王赛君,杨明,等.中药挥发油提取工艺与装备现状及问题分析[J].中国实验方剂学杂志,2014,20(14):224-228.
[16]郭立玮,邢卫红,朱华旭,等.中药膜技术的“绿色制造”特征、国家战略需求及其关键科学问题与应对策略[J].中草药,2017,48(16):3267-3279.
[17]陈翠仙,韩宾兵,朗宁·威.渗透蒸发和蒸汽渗透[M].北京:化学工业出版社,2004.
[18]伍利华,黄英,刘婷,等.陶瓷膜分离技术应用于中药口服液的研究进展[J].药物评价研究,2014,37(2):184-187.
[19]朱华旭,李益群,徐丽,等.陶瓷膜微滤过程中小檗碱与高分子物质相互作用的初步研究[J].中草药,2018,49(18):4250-4258.
[20]中国药典[S].四部.2015.
[21]朱华旭,郭立玮,潘永兰,等.一种提取柴胡挥发油的装置:中国,201520608397.2[P].2015-08-13.
[22]龙观洪.面向应用的中药挥发油的蒸汽渗透膜分离过程的初步研究[D].南京:南京中医药大学,2016.
[23]张淑蓉,裴晓丽,王华阳.不同采收期连翘挥发油中α-蒎烯和β-蒎烯含量的比较[J].中国药房,2013,24(47):4469-4471.
[24]樊文玲,郭立玮,林瑛,等.丁香等8种中药含油水体的溶液环境对体系通量和收油率影响的研究[J].中国中药杂志,2013,38(19):3277-3281.
[25]沈洁,韩志峰,郭立玮,等.超滤膜富集干姜挥发油的工艺优化研究[J].中草药,2012,43(8):1526-1530.
[26]龙观洪,李博,朱华旭,等.膜分离技术富集中药挥发油的可行性及其工艺过程初探-以中药青皮为例[J].膜科学与技术,2016,36(3):124-130.
[2]覃梦瑶,阮文懿,翟苑好,等.三伏贴中芥子碱及细辛挥发油促进HaCaT细胞摄取延胡索乙素的作用及其机制研究[J].中草药,2018,49(2):400-405.
[3]刘海燕,范婧,高微微,等.细辛挥发油对植物病原真菌的抑制作用研究[J].中草药,2007,38(12):1878-1881.
[4]Wang B,Yuan D.Comparative study of chemical composition,antinociceptive effect and acute toxicity of the essential oils of three Asarum drugs[J].J Chin Pharm Sci,2014,23(7):480-489.
[5]费可,胡瑜瑕,周璐,等.细辛临床应用与药理作用研究进展[J].上海中医药大学学报,2010,24(6):87-90.
[6]唐锋,梁少瑜,田元新,等.细辛挥发油抗过敏性鼻炎有效成分及靶点预测的研究[J].中国实验方剂学杂志,2015,21(24):126-131.
[7]刘东吉,刘春生.不同产地栽培辽细辛的挥发油研究[J].中国实验方剂学杂志,2010,16(9):79-82.
[8]杜成智,陈玉萍,覃洁萍,等.不同产地细辛挥发油的GC-MS分析[J].中国实验方剂学杂志,2011,17(7):57-59.
[9]梁学清,李丹丹.细辛药理作用研究进展[J].河南科技大学学报:医学版,2011,29(4):318-320.
[10]潘磊,郑柳,牛卉,等.细辛挥发油成分提取与甲基丁香酚含量测定的研究进展[J].时珍国医国药,2015,26(4):967-970.
[11]Senatore F,Arnold N A,Bruno M.Essential components of Centaurea eryngiodes Lam.and Centaurea iberica Trev.var.hermonis Bois.Lam.,two Asteraceae growing wild in Lebanon[J].Nat Prod Res,2005,19(8):749-754.
[12]陆钊,高凯,潘淑霞,等.加速溶剂萃取/气相色谱-质谱法分析朝鲜淫羊藿挥发油成分[J].哈尔滨工业大学学报,2011,43(8):145-148.
[13]Kim M R,El-Aty A M,Choi J H,et al.Identification of essential components in Angelica species using supercritical-CO2 fluid extraction and solid phase microextraction coupled to gas chromatography-mass spectrometry[J].Biomed Chromatogr,2006,20(11):1267-1273.
[14]何颖.中药挥发油提取方法分析[J].天津药学,2015,27(1):47-50.
[15]伍振峰,王赛君,杨明,等.中药挥发油提取工艺与装备现状及问题分析[J].中国实验方剂学杂志,2014,20(14):224-228.
[16]郭立玮,邢卫红,朱华旭,等.中药膜技术的“绿色制造”特征、国家战略需求及其关键科学问题与应对策略[J].中草药,2017,48(16):3267-3279.
[17]陈翠仙,韩宾兵,朗宁·威.渗透蒸发和蒸汽渗透[M].北京:化学工业出版社,2004.
[18]伍利华,黄英,刘婷,等.陶瓷膜分离技术应用于中药口服液的研究进展[J].药物评价研究,2014,37(2):184-187.
[19]朱华旭,李益群,徐丽,等.陶瓷膜微滤过程中小檗碱与高分子物质相互作用的初步研究[J].中草药,2018,49(18):4250-4258.
[20]中国药典[S].四部.2015.
[21]朱华旭,郭立玮,潘永兰,等.一种提取柴胡挥发油的装置:中国,201520608397.2[P].2015-08-13.
[22]龙观洪.面向应用的中药挥发油的蒸汽渗透膜分离过程的初步研究[D].南京:南京中医药大学,2016.
[23]张淑蓉,裴晓丽,王华阳.不同采收期连翘挥发油中α-蒎烯和β-蒎烯含量的比较[J].中国药房,2013,24(47):4469-4471.
[24]樊文玲,郭立玮,林瑛,等.丁香等8种中药含油水体的溶液环境对体系通量和收油率影响的研究[J].中国中药杂志,2013,38(19):3277-3281.
[25]沈洁,韩志峰,郭立玮,等.超滤膜富集干姜挥发油的工艺优化研究[J].中草药,2012,43(8):1526-1530.
[26]龙观洪,李博,朱华旭,等.膜分离技术富集中药挥发油的可行性及其工艺过程初探-以中药青皮为例[J].膜科学与技术,2016,36(3):124-130.