超声波法提取凌云白毫茶中的咖啡碱
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摘要
目的探讨超声波法提取凌云白毫茶中咖啡碱的最佳提取方案。方法采用正交实验方法提取凌云白毫茶中的咖啡碱。结果本次研究得知咖啡碱最高提取量为每5g茶叶含咖啡碱66mg,咖啡碱的收率达到1.32%。咖啡碱提取最佳条件为:95%乙醇浓度,30min超声时间,120W超声功率,25∶1液固比。结论凌云白毫茶中含有的咖啡碱成分可以通过超声波方法提取,该方法操作简单,无污染且纯度较高,所得最佳方案可应用于工业化生产中。
Objective To explore the optimal scheme for extracting caffeine from Lingyun pekoe tea by ultrasonic method.Methods The orthogonal experiment method was used to extract the caffeine from Lingyun pekoe tea.Results This study showed that the maximum amount of caffeine extracted from 5 g of tea was 66 mg,the yield of caffeine reached 1.32%.The optimal extraction conditions were:95% ethanol concentration,30 min ultrasonic time,120 Wultrasonic power,and the ratio of liquid to solid was 25∶1.Conclusion The caffeine component contained in Lingyun pekoe tea can be extracted by ultrasonic method.This method is simple and without pollution and with higher purity,and the best extraction scheme obtained in this study can be applied to industrial production.
Objective To explore the optimal scheme for extracting caffeine from Lingyun pekoe tea by ultrasonic method.Methods The orthogonal experiment method was used to extract the caffeine from Lingyun pekoe tea.Results This study showed that the maximum amount of caffeine extracted from 5 g of tea was 66 mg,the yield of caffeine reached 1.32%.The optimal extraction conditions were:95% ethanol concentration,30 min ultrasonic time,120 Wultrasonic power,and the ratio of liquid to solid was 25∶1.Conclusion The caffeine component contained in Lingyun pekoe tea can be extracted by ultrasonic method.This method is simple and without pollution and with higher purity,and the best extraction scheme obtained in this study can be applied to industrial production.
引文
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[10]李泽夏琼,马莹,庞秀清,等.高效液相色谱法测定四种常见茶叶以及减肥茶中咖啡因的含量[J].食品安全导刊,2018(6):147-149.
[11]周芳,朱亚伟,位翠杰.正交试验法从茶叶中提取咖啡因的工艺研究[J].安徽农业科学,2015,43(19):274-275.
[2]陈然,孟庆佳,刘海新,等.不同种类茶叶多酚及生物碱含量特点分析[J].食品科技,2018,43(5):72-76.
[3]王子浩,尹鹏,刘威,等.信阳地区栽种的9种茶树品种加工白茶成分及品质分析[J].食品研究与开发,2018,39(6):143-145,168.
[4]郭叶,郝鹤.从茶叶中提取咖啡因实验改进[J].包头医学院学报,2017,33(4):111-112,118.
[5]陆青兰,陆海峰,黄世稳,等.壮药黄根中多糖含量的测定[J].右江民族医学院学报,2018,40(5):405-408.
[6]林瑶,谭冰,韦文荣,等.不同提取法对广金钱草总黄酮抗氧化活性的影响研究[J].右江民族医学院学报,2016,38(4):364-367.
[7]曹雅丽.茶叶中咖啡因提取方法的比较研究[J].现代园艺,2018(12):13-14.
[8]闫洪军,郑择,李涵汶,等.茶叶中咖啡因的提取及条件优化[J].安徽化工,2018,44(4):36-37.
[9]黄飞,徐慧敏,王杰,等.Box-Behnken设计-效应面法优化离子液体提取废次茶叶中咖啡因工艺[J].天然产物研究与开发,2017,29(3):489-496.
[10]李泽夏琼,马莹,庞秀清,等.高效液相色谱法测定四种常见茶叶以及减肥茶中咖啡因的含量[J].食品安全导刊,2018(6):147-149.
[11]周芳,朱亚伟,位翠杰.正交试验法从茶叶中提取咖啡因的工艺研究[J].安徽农业科学,2015,43(19):274-275.