不同药物麻醉的乳腺癌患者血清对乳腺癌MDA-MB-231细胞凋亡的影响
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摘要
目的:探究不同药物麻醉的乳腺癌患者血清对体外乳腺癌MDA-MB-231细胞凋亡的影响。方法:收集我院2016年2月至2017年12月期间采用丙泊酚麻醉的乳腺癌患者血液样本28例,七氟醚麻醉的乳腺癌患者血液标本32例,乳腺癌术后镇痛使用曲马多的患者血液样本25例,未使用任何麻醉药的普通乳腺癌患者血液样本20例。将血清作用于乳腺癌MDA-MB-231细胞24 h后,采用流式细胞法检测细胞的凋亡率,ELISA法检测各组细胞中半胱氨酸蛋白酶3(caspase-3)和半胱氨酸蛋白酶8(caspase-8)的表达情况。结果:流式细胞法检测结果显示,使用丙泊酚、七氟醚麻醉和曲马多术后镇痛的患者血清均能够显著提高乳腺癌MDA-MB-231细胞凋亡率; ELISA结果显示,使用丙泊酚、七氟醚麻醉和曲马多术后镇痛的患者血清均能够显著提高乳腺癌MDA-MB-231细胞caspase-3的表达(P <0. 05),且各组之间无统计学差异(P> 0. 05);而使用丙泊酚和七氟醚麻醉的患者血清均能够显著提高乳腺癌MDA-MB-231细胞caspase-8的表达(P <0. 05),曲马多却未能影响乳腺癌MDA-MB-231细胞caspase-8的表达(P> 0. 05),丙泊酚和七氟醚组与曲马多组对比,差异具有统计学意义(P <0. 05)。结论:使用丙泊酚、七氟醚麻醉和曲马多术后镇痛的患者血清均能够显著提高乳腺癌MDA-MB-231细胞凋亡率及caspase-3的表达;而使用丙泊酚和七氟醚麻醉的患者血清能够显著提高乳腺癌MDA-MB-231细胞caspase-8的表达,曲马多对乳腺癌MDAMB-231细胞caspase-8的表达没有明显影响。
Objective: To investigate the effect of serum from patients with breast cancer with different drugs anesthesia on the apoptosis of breast cancer MDA-MB-231 cells in vitro. Methods: 28 blood samples from patients with breast cancer anesthetized with propofol,32 patients with sevoflurane anesthetized,blood samples of 25 patients after tramadol analgesia after breast cancer operation and 20 patients with normal breast cancer without any anesthetics from February 2016 to December 2017 in our hospital were collected. After sera were treated with MDA-MB-231 cells for 24 h,the apoptosis rate of the cells was detected by flow cytometry. Expression of caspase-3 and caspase-8 were detected by ELISA. Results: Flow cytometry results showed that the use of propofol,sevoflurane anesthesia and tramadol postoperative analgesia can significantly increase the apoptosis of breast cancer MDA-MB-231 cells. ELISA results show that the use of different the serum levels of propofol,sevoflurane anesthesia,and tramadol postoperative analgesia significantly increased the expression of caspase-3 in breast cancer MDA-MB-231 cells( P < 0. 05),and there was no statistical difference between the groups( P > 0. 05). Serum from patients anesthetized with propofol and sevoflurane significantly increased the expression of caspase-8 in breast cancer MDA-MB-231 cells( P < 0. 05),but tramadol failed to affect the expression of caspase-8 in breast cancer MDA-MB-231 cells( P > 0. 05),the difference between propofol and sevoflurane groups and tramadol group were statistically significant( P < 0. 05). Conclusion: Patients treated with propofol,sevoflurane anesthesia,and postoperative analgesia with tramadol significantly increased the rate of apoptosis and caspase-3 expression in breast cancer MDA-MB-231 cells. The serum of patients with propofol,sevoflurane anesthesia can significantly increase the expression of caspase-8 in breast cancer MDA-MB-231 cells. Tramadol has no significant effect on the expression of caspase-8 in breast cancer cells MDA-MB-231.
Objective: To investigate the effect of serum from patients with breast cancer with different drugs anesthesia on the apoptosis of breast cancer MDA-MB-231 cells in vitro. Methods: 28 blood samples from patients with breast cancer anesthetized with propofol,32 patients with sevoflurane anesthetized,blood samples of 25 patients after tramadol analgesia after breast cancer operation and 20 patients with normal breast cancer without any anesthetics from February 2016 to December 2017 in our hospital were collected. After sera were treated with MDA-MB-231 cells for 24 h,the apoptosis rate of the cells was detected by flow cytometry. Expression of caspase-3 and caspase-8 were detected by ELISA. Results: Flow cytometry results showed that the use of propofol,sevoflurane anesthesia and tramadol postoperative analgesia can significantly increase the apoptosis of breast cancer MDA-MB-231 cells. ELISA results show that the use of different the serum levels of propofol,sevoflurane anesthesia,and tramadol postoperative analgesia significantly increased the expression of caspase-3 in breast cancer MDA-MB-231 cells( P < 0. 05),and there was no statistical difference between the groups( P > 0. 05). Serum from patients anesthetized with propofol and sevoflurane significantly increased the expression of caspase-8 in breast cancer MDA-MB-231 cells( P < 0. 05),but tramadol failed to affect the expression of caspase-8 in breast cancer MDA-MB-231 cells( P > 0. 05),the difference between propofol and sevoflurane groups and tramadol group were statistically significant( P < 0. 05). Conclusion: Patients treated with propofol,sevoflurane anesthesia,and postoperative analgesia with tramadol significantly increased the rate of apoptosis and caspase-3 expression in breast cancer MDA-MB-231 cells. The serum of patients with propofol,sevoflurane anesthesia can significantly increase the expression of caspase-8 in breast cancer MDA-MB-231 cells. Tramadol has no significant effect on the expression of caspase-8 in breast cancer cells MDA-MB-231.
引文
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[17]Yang Liyun. Effect of dexmedetomidine on proliferation,migration and apoptosis of MCF-7 breast cancer cells[D]. Guangxi Medical University,2013.[阳丽云.右美托咪定对MCF-7乳腺癌细胞增殖、迁移和凋亡的影响[D].广西医科大学,2013.]
[18]Wang Jinjing,Sun Baocun. Expression and significance of Caspase-3 and Caspase-9 in breast cancer[J]. Journal of Clinical and Experimental Pathology,2012,28(4):378-381.[王进京,孙保存.乳腺癌中Caspase-3和Caspase-9的表达及其意义[J].临床与实验病理学杂志,2012,28(4):378-381.]
[2]Rossaint J. Propofol anesthesia and remote ischemic preconditioning:An unfortunate relationship[J]. Anesthesia and Analgesia,2018,126(4):1118-1120.
[3]Araki R,Hayashi K,Sawa T. Dopamine D2-receptor antagonist droperidol deepens sevoflurane anesthesia[J]. Anesthesiology,2018,128(4):754-763.
[4]Chen Y,Liang M,Zhu Y,et al. The effect of propofol and sevoflurane on the perioperative immunity in patients under laparoscopic radical resection of colorectal cancer[J]. National Medical Journal of China,2015,95(42):3440-3444.
[5]Wenjing Yang,Jun Cai,Catherine Zabkiewicz,et al. The effects of anesthetics on recurrence and metastasis of cancer,and clinical implications[J]. World Journal of Oncology,2017,8(3):63-70.
[6]Bang JY,Lee JB,Oh J,et al. The influence of propofol and sevoflurane on acute kidney injury after colorectal surgery:A retrospective cohort study[J]. Anesthesia&Analgesia,2016,123(2):363-370.
[7]Chang Hailin. Effect of sevoflurane on hemodynamics of patients undergoing radical mastectomy for breast cancer[J]. Primary Health Care in China,2015,29(12):106-107.[常海林.七氟醚对乳腺癌根治术喉罩吸入麻醉患者血流动力学的影响[J].中国初级卫生保健,2015,29(12):106-107.]
[8]Liu Jian. Comparison of the effects of propofol and sevoflurane in anesthesia for patients with malignant tumors[J]. Modern Chinese Medicine,2016,10(04):146-147.[刘键.丙泊酚与七氟醚在恶性肿瘤疾病患者麻醉中的效果比较[J].中国现代药物应用,2016,10(04):146-147.]
[9]Xu Lanhua,Li Shuping,Wang Mei,et al. Anesthetic comparison of sevoflurane and propofol combined with remifentanil for radical mastectomy[J]. Heilongjiang Medical Science,2012,35(05):20-22.[徐兰华,李淑萍,王梅,等.七氟醚与丙泊酚复合瑞芬太尼用于乳癌根治术的麻醉比较[J].黑龙江医药科学,2012,35(05):20-22.]
[10]Chen X,Wu Q,You L,et al. Propofol attenuates pancreatic cancer malignant potential via inhibition of NMDA receptor[J]. Eur J Pharmacol,2017,795:150-159.
[11]Li H,Lu Y,Pang Y,et al. Propofol enhances the cisplatin-induced apoptosis on cervical cancer cells via EGFR/JAK2/STAT3pathway[J]. Biomedicine&Pharmacotherapy,2017,86:324-333.
[12]Ou W,Lv J,Zou X,et al. Propofol inhibits hepatocellular carcinoma growth and invasion through the HMGA2-mediated Wnt/beta-catenin pathway[J]. Exp Ther Med,2017,13(5):2501-2506.
[13]Kim R,Kawai A,Wakisaka M,et al. Differences in immune response to anesthetics used for day surgery versus hospitalization surgery for breast cancer patients[J]. Clinical and Translational Medicine,2017,6(1):34.
[14]Tian HT,Duan XH,Yang YF,et al. Effects of propofol or sevoflurane anesthesia on the perioperative inflammatory response,pulmonary function and cognitive function in patients receiving lung cancer resection[J]. European Review for Medical and Pharmacological Sciences,2017,21(23):5515-5522.
[15]Feng Juan,Wu Manwu. Effects of isoflurane on human breast cancer MDA-MB-231 cells and related mechanisms[J].Modern Chinese Doctor,2017,55(05):8-11.[冯娟,吴满武.异氟醚对人乳腺癌MDA-MB-231细胞的影响及相关机制探讨[J].中国现代医生,2017,55(05):8-11.]
[16]Cheng K,Giebaly D,Campbell A,et al. Systemic effects of polymethylmethycrylate in total knee replacement:A prospective casecontrol study[J]. Bone Joint Res,2014,3(4):108-116.
[17]Yang Liyun. Effect of dexmedetomidine on proliferation,migration and apoptosis of MCF-7 breast cancer cells[D]. Guangxi Medical University,2013.[阳丽云.右美托咪定对MCF-7乳腺癌细胞增殖、迁移和凋亡的影响[D].广西医科大学,2013.]
[18]Wang Jinjing,Sun Baocun. Expression and significance of Caspase-3 and Caspase-9 in breast cancer[J]. Journal of Clinical and Experimental Pathology,2012,28(4):378-381.[王进京,孙保存.乳腺癌中Caspase-3和Caspase-9的表达及其意义[J].临床与实验病理学杂志,2012,28(4):378-381.]
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