血清高迁移率族蛋白B1、可溶性CD40L、脂肪型脂肪酸结合蛋白和髓过氧化物酶在重度子痫前期患者中的表达及意义
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摘要
目的探讨血清高迁移率族蛋白B1 (HMGB1)、可溶性CD40L (sCD40L)、脂肪型脂肪酸结合蛋白(FABP4)、髓过氧化物酶(MPO)在重度子痫前期患者中的表达及意义,为临床诊断和病情评估提供参考依据。方法 2016年1-12月,选择67例重度子痫前期患者,其中早发型组28例和晚发型组39例以及同期正常妊娠者60例作为对照组。采用酶联免疫吸附法检测血清HMGB1、sCD40L、FABP4和MPO的表达水平。结果重度子痫前期组患者血清HMGB1、sCD40L、FABP4和MPO水平为(76. 23±21. 59) mmol/L、(6. 37±2. 23) ng/L、(158. 61±36. 68)μg/L、(15. 69±1. 28) ng/L,而对照组为(26. 39±6. 38) mmol/L、(3. 25±1. 26) ng/L、(89. 76±11. 39)μg/L、(11. 29±0. 62) ng/L,两组比较差异有统计学意义(均P<0. 05)。早发型组血清HMGB1、sCD40L、FABP4和MPO均高于晚发型组,两组比较差异有统计学意义(均P<0. 05)。四项指标联合检测的ROC曲线下面积为0. 859,明显高于HMGB1、sCD40L、FABP4和MPO各个指标单独检测,差异有统计学意义(Z=7. 861,8. 061,8. 672,7. 541,均P<0. 001)。Pearson积差相关性显示,重度子痫前期患者中HMGB1、sCD40L、FABP4和MPO表达均呈显著相关性,差异有统计学意义(P<0. 01)。结论血清HMGB1、sCD40L、FABP4和MPO的表达与重度子痫前期发生、发展密切相关,可作为血清标志物用于重度子痫前期的诊断和病情评估。
Objective To explore the expression and significance of serum high mobility group box protein B1 (HMGB1),soluble CD40 L (sCD40L),fatty acid binding protein 4 (FABP4),and myeloperoxidase (MPO) in severe preeclampsia patients,provide a reference basis for clinical diagnosis and evaluation of the disease. Methods From January to December in 2016,67 severe preeclampsia patients were selected and divided into early-onset preeclampsia group (28 patients) and late-onset preeclampsia group (39 patients),60 normal pregnant women during the same period were selected as control group. ELISA was used to detect the expression levels of serum HMGB1,sCD40L,FABP4,and MPO. Results The levels of serum HMGB1,sCD40L,FABP4,and MPO in severe preeclampsia group were (76. 23±21. 59) mmol/L, (6. 37±2. 23) ng/L, (158. 61±36. 68) μg/L,and (15. 69±1. 28) ng/L,respectively. The levels of serum HMGB1,sCD40L,FABP4,and MPO in control group were (26. 39± 6. 38) mmol/L, (3. 25± 1. 26) ng/L, (89. 76± 11. 39) μg/L,and (11. 29±0. 62) ng/L,respectively. There were statistically significant differences in the levels of serum HMGB1,sCD40L,FABP4,and MPO between the two groups (P<0. 05). The levels of serum HMGB1,sCD40L,FABP4,and MPO in early-onset preeclampsia group were statistically significantly higher than those in late-onset preeclampsia group (P<0. 05). The area under ROC of combination detection of four parameters was 0. 859,which was statistically significantly higher than those of HMGB1,sCD40L,FABP4,and MPO,respectively (Z= 7. 861,8. 061,8. 672,7. 541,P<0. 001). Pearson product-moment correlation showed that there were significant correlations among the four parameters (P<0. 01). Conclusion The expressions of serum HMGB1,sCD40L,FABP4,and MPO are closely correlated with onset and development of severe preeclampsia,which can be used for diagnosis and disease assessment of severe preeclampsia as serum markers.
Objective To explore the expression and significance of serum high mobility group box protein B1 (HMGB1),soluble CD40 L (sCD40L),fatty acid binding protein 4 (FABP4),and myeloperoxidase (MPO) in severe preeclampsia patients,provide a reference basis for clinical diagnosis and evaluation of the disease. Methods From January to December in 2016,67 severe preeclampsia patients were selected and divided into early-onset preeclampsia group (28 patients) and late-onset preeclampsia group (39 patients),60 normal pregnant women during the same period were selected as control group. ELISA was used to detect the expression levels of serum HMGB1,sCD40L,FABP4,and MPO. Results The levels of serum HMGB1,sCD40L,FABP4,and MPO in severe preeclampsia group were (76. 23±21. 59) mmol/L, (6. 37±2. 23) ng/L, (158. 61±36. 68) μg/L,and (15. 69±1. 28) ng/L,respectively. The levels of serum HMGB1,sCD40L,FABP4,and MPO in control group were (26. 39± 6. 38) mmol/L, (3. 25± 1. 26) ng/L, (89. 76± 11. 39) μg/L,and (11. 29±0. 62) ng/L,respectively. There were statistically significant differences in the levels of serum HMGB1,sCD40L,FABP4,and MPO between the two groups (P<0. 05). The levels of serum HMGB1,sCD40L,FABP4,and MPO in early-onset preeclampsia group were statistically significantly higher than those in late-onset preeclampsia group (P<0. 05). The area under ROC of combination detection of four parameters was 0. 859,which was statistically significantly higher than those of HMGB1,sCD40L,FABP4,and MPO,respectively (Z= 7. 861,8. 061,8. 672,7. 541,P<0. 001). Pearson product-moment correlation showed that there were significant correlations among the four parameters (P<0. 01). Conclusion The expressions of serum HMGB1,sCD40L,FABP4,and MPO are closely correlated with onset and development of severe preeclampsia,which can be used for diagnosis and disease assessment of severe preeclampsia as serum markers.
引文
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[15]杨秀凤,位菊峰,张文华.重度子痫前期病人血清FABP4的表达及意义[J].青岛大学医学院学报,2016,52(5):595-597.
[16]Pulli B,Wojtkiewicz G,Iwamoto Y,et al.Molecular MR imaging of myeloperoxidase distinguishes steatosis from steatohepatitis in nonalcoholic fatty liver disease[J].Radiology,2017,284(2):390-400.
[17]蒋惠玲,胥保梅,许颖.孕妇血清、脐血及胎盘组织中髓过氧化物酶水平和子痫前期发病的关系[J].中国妇幼保健,2016,31(19):3913-3915.
[2]Sircar M,Thadhani R,Karumanchi SA.Pathogenesis of preeclampsia[J].Curr Opin Nephrol Hypertens,2015,24(2):131-138.
[3]Smith TA,Kirkpatrick DR,Kovilam O,et al.Immunomodulatory role of vitamin D in the pathogenesis of preeclampsia[J].Expert Rev Clin Immunol,2015,11(9):1055-1063.
[4]刘扬,龚作炯.高迁移率族蛋白B1与肝脏疾病关系的研究进展[J].疑难病杂志,2017,16(9):959-962.
[5]Foglio E,Puddighinu G,Germani A,et al.HMGB1 inhibits apoptosis following MI and induces autophagy via m TORC1 inhibition[J].J Cell Physiol,2017,232(5):1135-1143.
[6]Tang L,Chai W,Ye F,et al.HMGB1 promotes differentiation syndrome by inducing hyperinflammation via MEK/ERK signaling in acute promyelocytic leukemia cells[J].Oncotarget,2017,8(16):27314-27327.
[7]金雅芳,曹敏恺,邹金芳.高迁移率族蛋白B1与子痫前期的相关性研究[J].现代医学,2015,43(3):349-351.
[8]郭凯,冯亚玲,赵敏,等.高迁移率族蛋白B1、白细胞介素-6和转化生长因子-β1在重度子痫前期患者外周血中的表达及意义[J].中国妇幼保健,2017,32(10):2065-2068.
[9]Xie JX,Alderson H,Ritchie J,et al.Circulating CD40 and scd40l predict changes in renal function in subjects with chronic kidney disease[J].Sci Rep,2017,7(1):7942.
[10]Simic D,Bogdan N,Teng F,et al.Blockingα5β1 integrin attenuates s CD40L-mediated platelet activation[J].Clin Appl Thromb Hemost,2017,23(6):607-614.
[11]周进福,朱文斌,罗金英,等.重度子痫前期患者血清可溶性CD40L的表达水平与意义[J].临床检验杂志,2014,32(3):204-206.
[12]Yan F,Shen N,Pang JX,et al.Fatty acid-binding protein FABP4 mechanistically links obesity with aggressive AML by enhancing aberrant DNA methylation in AML cells[J].Leukemia,2017,31(6):1434-1442.
[13]Guaita-Esteruelas S,Bosquet A,Saavedra P,et al.Exogenous FABP 4 increases breast cancer cell proliferation and activates the expression of fatty acid transport proteins[J].Mol Carcinog,2017,56(1):208-217.
[14]Harjes U,Bridges E,Gharpure KM,et al.Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4[J].Oncogene,2017,36(7):912-921.
[15]杨秀凤,位菊峰,张文华.重度子痫前期病人血清FABP4的表达及意义[J].青岛大学医学院学报,2016,52(5):595-597.
[16]Pulli B,Wojtkiewicz G,Iwamoto Y,et al.Molecular MR imaging of myeloperoxidase distinguishes steatosis from steatohepatitis in nonalcoholic fatty liver disease[J].Radiology,2017,284(2):390-400.
[17]蒋惠玲,胥保梅,许颖.孕妇血清、脐血及胎盘组织中髓过氧化物酶水平和子痫前期发病的关系[J].中国妇幼保健,2016,31(19):3913-3915.