油藏环境与物理模型中的微生物群落比较研究
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摘要
为揭示模拟油藏环境的岩心与真实油藏的微生物群落结构之间存在的差异程度,应用高通量分析方法,一方面分析具体油藏沾3区块注入水和6口油井的产出液,另一方面分析模拟沾3区块油藏条件的物模实验岩心产出液,对比分析注入水、油井产出液和物模实验岩心产出液三者细菌及古菌的群落结构.结果显示,同一油藏不同油井之间细菌群落结构差异明显,每口油井在不同时间其优势细菌变化较大,常见的细菌有Desulfacinum、Thermodesulforhabdus、Pseudomonas等属;而古菌群落结构保持相对稳定,常见的古菌有Methanosaeta和Methanomethylovorans等属.将注入水、油藏产出液和岩心产出液三者所有的样品分析结果在属水平上进行相对含量排序,对比三者细菌和古菌排序前15个属,结果表明油藏和岩心中的细菌群落结构差异明显,仅有4个共有属,分别是Pseudomonas、Syntrophomonas、Petrobacter和Acinetobacter,而两者主要古菌群落结构非常相近.本研究说明物理模型还不能完全模拟真实油藏,只能部分接近油藏环境.(图9表1参19)
This study aimed to reveal the difference between the microbial community structure of the reservoir environment and physical model environment of a simulated reservoir. The microbial community structures of the injection water and the produced fluid of the ‘Zhan3' block in the Shengli Oil Field, and the model output fluid were analyzed by a high-throughput sequencing technology. The results showed that there are apparent differences in bacterial community structure between different wells in the same block. The dominant bacteria also changed considerably in the same well at different sampling times. The common bacterial species in the Zhan3 oil reservoir are, among others, Desulfacinum sp., Thermodesulforhabdus sp., and Pseudomonas sp. Contrarily, the Archaea community structure in this block is relatively stable, mainly containing Methanosaeta sp. and Methanomethylovorans sp. The microbial community structures of the reservoir injection water, the oil well output fluid, and the physical model output fluid were recorded to genera-level. The 15 most abundant microorganisms were selected for comparison. The results showed that the bacterial community structures of the reservoir and the core samples were distinct: they only had four genera in common; Pseudomonas sp., Syntrophomonas sp., Petrobacter sp., and Acinetobacter sp. Their Archaea community structure, however, is similar. The results of this study confirmed that the physical model can approximate the reservoir conditions, but cannot adequately simulate the reservoir environment.
This study aimed to reveal the difference between the microbial community structure of the reservoir environment and physical model environment of a simulated reservoir. The microbial community structures of the injection water and the produced fluid of the ‘Zhan3' block in the Shengli Oil Field, and the model output fluid were analyzed by a high-throughput sequencing technology. The results showed that there are apparent differences in bacterial community structure between different wells in the same block. The dominant bacteria also changed considerably in the same well at different sampling times. The common bacterial species in the Zhan3 oil reservoir are, among others, Desulfacinum sp., Thermodesulforhabdus sp., and Pseudomonas sp. Contrarily, the Archaea community structure in this block is relatively stable, mainly containing Methanosaeta sp. and Methanomethylovorans sp. The microbial community structures of the reservoir injection water, the oil well output fluid, and the physical model output fluid were recorded to genera-level. The 15 most abundant microorganisms were selected for comparison. The results showed that the bacterial community structures of the reservoir and the core samples were distinct: they only had four genera in common; Pseudomonas sp., Syntrophomonas sp., Petrobacter sp., and Acinetobacter sp. Their Archaea community structure, however, is similar. The results of this study confirmed that the physical model can approximate the reservoir conditions, but cannot adequately simulate the reservoir environment.
引文
1胡婧,曹功泽,王文杰,吴晓玲,曹嫣镔,汪卫东.沾3内源微生物驱的生物特征变化及其对驱油效果的影响[J].中国石油大学学报(自然科学版),2017,41(4):174-179[Hu J,Cao GZ,Wang WJ,Wu XL,Cao YB,Wang WD.Change of biological features and its impact on flooding effect of indigenous MEOR in Zhan3 block[J].J China Univ Petrol(Ed Nat Sci),2017,41(4):174-179]
2汪卫东.微生物采油技术研究及试验[J].石油钻采工艺,2012,34(1):107-113[Wang WD.Laboratory research and field trials of microbial oil recovery technique[J].Oil Drilling Prod Technol,2012,34(1):107-113]
3 Ibrahimov KM,Guseynova NI,Abdullayeva FY.Experience of microbial enhanced oil recovery methods at Azerbaijan Fields[J].SPE,2015:10.2118/177377-MS
4汪卫东,魏斌,谭云贤,王修林.微生物采油需要进一步解决的问题[J].石油勘探与开发,2004,31(6):107-113[Wang WD,Wei B,Tan YX,Wang XL.Problems confronted in microbial enhanced oil recovery[J].Petrol Explor Dev,2004,31(6):107-113]
5 Orphan VJ,Taylor LT,Hafenbradl D.Culture-dependent and cultureindependent characterization of microbial assemblages associated with high-temperature petroleum reservoirs[J].Appl Environ Microbiol,2000,66(2):700-711
6 Bonch-Osmolovskaya EA,Miroshnichenko ML,Lebedinsky AV.Radioisotopic,culture-based,and oligonu cleotide microchip analyses of thermophilic microbial communities in a continental high-temperature petroleum reservoir[J].Appl Environ Microbial,2003,69:6143-6151
7 Nazina TN,Shestakova NM,Grigor’Yan AA.Phylogenetic diversity and activity of anaerobic microorganisms of high-temperature horizons of the Dagang oil field(P.R.China)[J].Mikrobiologiia,2006,75(1):55-65
8 Hughes DS.Revival of microbial enhanced oil recover y(MEOR)initiatives on UK continental shelf[A]//24th Annual Workshop&Sy mposium Collaborative Project on En hanced Oil Recover y International Energy Agency,Vienna,2003
9李彩风,李阳,曹嫣镔,宋永亭,李希明,汪卫东,包木太.油藏环境产脂肽类表面活性剂微生物的分布[J].石油学报,2015,36(9):1122-1126[Li CF,LI Y,Cao YB,Song YT,Li XM,Wang WD,Bao MT.Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment[J].Acta Petrol Sin,2015,36(9):1122-1126]
10 Youssef N,SimpsonDR,Duncan KE,Mcinerney MJ,Folmsbee M.Insitu biosurfactant production by injected Bacillus strains in a limestone petroleum reservoir[J].Appl Environ Microbiol,2007,73(4):1239-1247
11宋永亭.不同配气条件下微生物驱油生长代谢规律[J].应用与环境生物学报,2017,23(6):974-978[Song YT.Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions[J].Chin J Appl Environ Biol,2017,23(6):974-978]
12曹功泽,巴燕,刘涛,毕爱文,姚寒梅.沾3区块内源微生物驱油现场试验[J].特种油气藏,2014,21(1):145-147[Cao GZ,Ba Y,Liu T,Bi AW,Yao HM.Field pilot test of indigenous microbial flooding in Block Zhan 3[J].Special Oil Gas Reserv,2014,21(1):145-147]
13孙刚正,宋欣,吴晓玲,汪卫东,李希明,王增林.不同长效激活剂组分对沾3区块内源功能微生物激活研究[J].西安石油大学学报(自然科学版),2017,32(6):112-118[Sun GZ,Song X,Wu XL,Wang WD,Li XM,Wang ZL.Study on activation of different long-acting activators on endogenous functional microorganisms in Zhan 3 Block[J].J Xi’an Shiyou Univ(Nat Sci Ed),2017,32(6):112-118]
14 Nazina TN,SokolovaDSh,Shestakova NM.The phylogenetic diversity of aerobic organotrophic bacteria from the Dagang high-temperature oil field[J].Microbiology,2005,74(3):343-351
15薛燕芬,于波,马延和.油田系统应用微生物学与分子生物学[M].北京:化学工业出版社,2015:29[Xue YF,Yu T,Ma YH.Applied Microbiology and Molecular Biology in Oilfield Systems[M].Beijing:Chemical Industry Press,2015]
16胡婧,刘涛.激活剂用量对油藏内源微生物群落的影响[J].生物加工过程,2016,14(3):7-11[Hu J,Liu T.Effect of activator amount on endogenous microbial community in reservoirs[J].Chin J Bioproc Eng,2016,14(3):7-11]
17宋永亭,胡婧,吴晓玲.室温条件下油藏采出液微生物群落结构稳定性[J].应用与环境生物学报,2017,23(3):495-501[Song YT,Hu J,Wu XL.Stability of microbial community structure in reservoir water samples at room temperature[J].Chin J Appl Environ Biol,2017,23(3):495-501]
18王慧,宋智勇,郝滨,刘涛,高光军,郭省学,汪卫东.微生物驱产出液群落结构与现场生产动态的关系[J].石油学报,2013,34(3):535-539[Wang H,Song ZY,Hao B,Liu T,Gao GJ,Guo SX,Wang WD.Relationship between structures of microbial colony in produced fluids and oil production performance in microbial flooding oil field[J].Acta Petrol Sin,2013,34(3):535-539]
19曹功泽,徐登霆,张绍东,巴燕,刘涛,孙刚正.胜利油田沾3断块内源微生物现场激活试验及分析[J].石油天然气学报,2012,34(7):136-140[Cao GZ,Xu DT,Zhang SD,Ba Y,Liu T,Sun GZ.Experiment and analysis of indigenous microbe activation in Block Zhan 3 in Shengli Oilfield[J].J Oil Gas Technol,2012,34(7):136-140]
2汪卫东.微生物采油技术研究及试验[J].石油钻采工艺,2012,34(1):107-113[Wang WD.Laboratory research and field trials of microbial oil recovery technique[J].Oil Drilling Prod Technol,2012,34(1):107-113]
3 Ibrahimov KM,Guseynova NI,Abdullayeva FY.Experience of microbial enhanced oil recovery methods at Azerbaijan Fields[J].SPE,2015:10.2118/177377-MS
4汪卫东,魏斌,谭云贤,王修林.微生物采油需要进一步解决的问题[J].石油勘探与开发,2004,31(6):107-113[Wang WD,Wei B,Tan YX,Wang XL.Problems confronted in microbial enhanced oil recovery[J].Petrol Explor Dev,2004,31(6):107-113]
5 Orphan VJ,Taylor LT,Hafenbradl D.Culture-dependent and cultureindependent characterization of microbial assemblages associated with high-temperature petroleum reservoirs[J].Appl Environ Microbiol,2000,66(2):700-711
6 Bonch-Osmolovskaya EA,Miroshnichenko ML,Lebedinsky AV.Radioisotopic,culture-based,and oligonu cleotide microchip analyses of thermophilic microbial communities in a continental high-temperature petroleum reservoir[J].Appl Environ Microbial,2003,69:6143-6151
7 Nazina TN,Shestakova NM,Grigor’Yan AA.Phylogenetic diversity and activity of anaerobic microorganisms of high-temperature horizons of the Dagang oil field(P.R.China)[J].Mikrobiologiia,2006,75(1):55-65
8 Hughes DS.Revival of microbial enhanced oil recover y(MEOR)initiatives on UK continental shelf[A]//24th Annual Workshop&Sy mposium Collaborative Project on En hanced Oil Recover y International Energy Agency,Vienna,2003
9李彩风,李阳,曹嫣镔,宋永亭,李希明,汪卫东,包木太.油藏环境产脂肽类表面活性剂微生物的分布[J].石油学报,2015,36(9):1122-1126[Li CF,LI Y,Cao YB,Song YT,Li XM,Wang WD,Bao MT.Distribution of the lipopeptide biosurfactant-producing microbes in oil reservoir environment[J].Acta Petrol Sin,2015,36(9):1122-1126]
10 Youssef N,SimpsonDR,Duncan KE,Mcinerney MJ,Folmsbee M.Insitu biosurfactant production by injected Bacillus strains in a limestone petroleum reservoir[J].Appl Environ Microbiol,2007,73(4):1239-1247
11宋永亭.不同配气条件下微生物驱油生长代谢规律[J].应用与环境生物学报,2017,23(6):974-978[Song YT.Regulation of microbial growth and metabolism and oil displacement under different gas distribution conditions[J].Chin J Appl Environ Biol,2017,23(6):974-978]
12曹功泽,巴燕,刘涛,毕爱文,姚寒梅.沾3区块内源微生物驱油现场试验[J].特种油气藏,2014,21(1):145-147[Cao GZ,Ba Y,Liu T,Bi AW,Yao HM.Field pilot test of indigenous microbial flooding in Block Zhan 3[J].Special Oil Gas Reserv,2014,21(1):145-147]
13孙刚正,宋欣,吴晓玲,汪卫东,李希明,王增林.不同长效激活剂组分对沾3区块内源功能微生物激活研究[J].西安石油大学学报(自然科学版),2017,32(6):112-118[Sun GZ,Song X,Wu XL,Wang WD,Li XM,Wang ZL.Study on activation of different long-acting activators on endogenous functional microorganisms in Zhan 3 Block[J].J Xi’an Shiyou Univ(Nat Sci Ed),2017,32(6):112-118]
14 Nazina TN,SokolovaDSh,Shestakova NM.The phylogenetic diversity of aerobic organotrophic bacteria from the Dagang high-temperature oil field[J].Microbiology,2005,74(3):343-351
15薛燕芬,于波,马延和.油田系统应用微生物学与分子生物学[M].北京:化学工业出版社,2015:29[Xue YF,Yu T,Ma YH.Applied Microbiology and Molecular Biology in Oilfield Systems[M].Beijing:Chemical Industry Press,2015]
16胡婧,刘涛.激活剂用量对油藏内源微生物群落的影响[J].生物加工过程,2016,14(3):7-11[Hu J,Liu T.Effect of activator amount on endogenous microbial community in reservoirs[J].Chin J Bioproc Eng,2016,14(3):7-11]
17宋永亭,胡婧,吴晓玲.室温条件下油藏采出液微生物群落结构稳定性[J].应用与环境生物学报,2017,23(3):495-501[Song YT,Hu J,Wu XL.Stability of microbial community structure in reservoir water samples at room temperature[J].Chin J Appl Environ Biol,2017,23(3):495-501]
18王慧,宋智勇,郝滨,刘涛,高光军,郭省学,汪卫东.微生物驱产出液群落结构与现场生产动态的关系[J].石油学报,2013,34(3):535-539[Wang H,Song ZY,Hao B,Liu T,Gao GJ,Guo SX,Wang WD.Relationship between structures of microbial colony in produced fluids and oil production performance in microbial flooding oil field[J].Acta Petrol Sin,2013,34(3):535-539]
19曹功泽,徐登霆,张绍东,巴燕,刘涛,孙刚正.胜利油田沾3断块内源微生物现场激活试验及分析[J].石油天然气学报,2012,34(7):136-140[Cao GZ,Xu DT,Zhang SD,Ba Y,Liu T,Sun GZ.Experiment and analysis of indigenous microbe activation in Block Zhan 3 in Shengli Oilfield[J].J Oil Gas Technol,2012,34(7):136-140]
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