IgA肾病动物模型研究现状
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摘要
目前普遍认为IgA肾病与遗传因素、免疫因素等有关,其动物模型尚无统一的造模方法。免疫复合诱导建立动物模型的方法操作简单,易于重复,经济适用,但存在较高的失败风险,且免疫因素造成的损伤需要与动物自身的恢复能力对抗,模型病情的轻重与动物的适应性、耐受性等有较大关系,且造模所需的时间一般较长。自发性IgA肾病动物模型临床与病理表现确定,出现IgA肾病的时间大多固定,大鼠的发病时间较早,造模失败的风险较低,与遗传因素有关,这与人类IgA肾病的家族聚集特点类似。此外,动物模型的建立还需要熟练的操作手法,稳定的饲养环境以及合理的饮食。
At present,it is generally believed that IgA nephropathy is related to genetic factors,immune factors and so on. There is no unified method for establishing animal models of IgA nephropathy. The method of establishing animal model by immune complex induction is simple,easy to repeat,economical and applicable,but it has high risk of failure,and the damage caused by immune factors needs to be confronted with the recovery ability of the animal itself. The severity of the disease of the model is closely related to the adaptability and tolerance of the animal,and the time needed to build the model is generally longer. The clinical and pathological manifestations of spontaneous IgA nephropathy animal model have been confirmed. Most of the time of IgA nephropathy has been fixed. Rats have earlier onset of IgA nephropathy,and the risk of model failure is lower. It is related to genetic factors,which is similar to the family clustering characteristics of human IgA nephropathy. In addition,the establishment of animal models also requires skilled manipulation,stable feeding environment,and a reasonable diet.
At present,it is generally believed that IgA nephropathy is related to genetic factors,immune factors and so on. There is no unified method for establishing animal models of IgA nephropathy. The method of establishing animal model by immune complex induction is simple,easy to repeat,economical and applicable,but it has high risk of failure,and the damage caused by immune factors needs to be confronted with the recovery ability of the animal itself. The severity of the disease of the model is closely related to the adaptability and tolerance of the animal,and the time needed to build the model is generally longer. The clinical and pathological manifestations of spontaneous IgA nephropathy animal model have been confirmed. Most of the time of IgA nephropathy has been fixed. Rats have earlier onset of IgA nephropathy,and the risk of model failure is lower. It is related to genetic factors,which is similar to the family clustering characteristics of human IgA nephropathy. In addition,the establishment of animal models also requires skilled manipulation,stable feeding environment,and a reasonable diet.
引文
[1]陈峰,刘学永,甘志洲,等.黄芪注射液穴位注射治疗Ig A肾病蛋白尿的临床研究[J].河北中医,2014,36(12):1852-1853.
[2] IMAI H,NAKAMOTO Y,ASAKURA K,et al. Spontaneous glomerular Ig A deposition in ddY mice:an animal model of Ig A nephritis[J]. Kidney Int,1985,27(5):756-761.
[3] OKAZAKI K,SUZUKI Y,OTSUJI M,et al. Development of a model of early-onset Ig A nephropathy[J]. J Am Soc Nephrol,2012,23(8):1364-1374.
[4] BERTHELOT L,PAPISTA C,MACIEL TT,et al. Transglutaminase is essential for Ig A nephropathy development acting through Ig A receptors[J]. J Exp Med,2012,209(4):793-806.
[5]王乾了,李贵森.动物模型与Ig A肾病的发病机制研究[J].中华肾病研究电子杂志,2014,3(2):77-81.
[6]万洁,许崇泰.两种方法复制家兔急性肾衰模型的对比研究[J].科技创新导报,2010,7(14):6-8.
[7]陶琦.肾脏病动物模型对中西医结合临床治疗指导作用的研究[D].南京:南京中医药大学,2013.
[8]李根林,张娟,张振凌,等.以腺嘌呤对慢性肾功能衰竭尿毒症期大鼠造模的方法研究[J].河南中医,2010,30(5):445-446.
[9]费梅,熊佩华,周丽霞,等.两种Ig A肾病大鼠模型的比较[J].江苏医药,2016,42(13):1435-1438.
[10]邹佳楠. PPAR-γ在Ig A肾病发生中的作用及其机理研究[D].上海:复旦大学,2014.
[11]李冬.大鼠肝衰竭前期模型建立及辅助性T淋巴细胞17/调节性T淋巴细胞失衡的研究[J].临床肝胆病杂志,2016,32(5):928-932.
[12]高明,张连栋,张莉,等.改良大鼠Ig A肾病模型建立及氧化应激状态评估[J].临床肾脏病杂志,2018,18(4):243-247.
[2] IMAI H,NAKAMOTO Y,ASAKURA K,et al. Spontaneous glomerular Ig A deposition in ddY mice:an animal model of Ig A nephritis[J]. Kidney Int,1985,27(5):756-761.
[3] OKAZAKI K,SUZUKI Y,OTSUJI M,et al. Development of a model of early-onset Ig A nephropathy[J]. J Am Soc Nephrol,2012,23(8):1364-1374.
[4] BERTHELOT L,PAPISTA C,MACIEL TT,et al. Transglutaminase is essential for Ig A nephropathy development acting through Ig A receptors[J]. J Exp Med,2012,209(4):793-806.
[5]王乾了,李贵森.动物模型与Ig A肾病的发病机制研究[J].中华肾病研究电子杂志,2014,3(2):77-81.
[6]万洁,许崇泰.两种方法复制家兔急性肾衰模型的对比研究[J].科技创新导报,2010,7(14):6-8.
[7]陶琦.肾脏病动物模型对中西医结合临床治疗指导作用的研究[D].南京:南京中医药大学,2013.
[8]李根林,张娟,张振凌,等.以腺嘌呤对慢性肾功能衰竭尿毒症期大鼠造模的方法研究[J].河南中医,2010,30(5):445-446.
[9]费梅,熊佩华,周丽霞,等.两种Ig A肾病大鼠模型的比较[J].江苏医药,2016,42(13):1435-1438.
[10]邹佳楠. PPAR-γ在Ig A肾病发生中的作用及其机理研究[D].上海:复旦大学,2014.
[11]李冬.大鼠肝衰竭前期模型建立及辅助性T淋巴细胞17/调节性T淋巴细胞失衡的研究[J].临床肝胆病杂志,2016,32(5):928-932.
[12]高明,张连栋,张莉,等.改良大鼠Ig A肾病模型建立及氧化应激状态评估[J].临床肾脏病杂志,2018,18(4):243-247.
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