接种好氧硝化污泥的CRI滤池厌氧氨氧化启动性能研究
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摘要
为解决CRI滤池因碳源缺乏导致的TN去除效率低的问题,考察了接种好氧硝化污泥后CRI滤池启动厌氧氨氧化的可行性及启动性能。结果表明,经过适应期(1～20 d)、活性迟滞期(21～49 d)、活性提高期(50～132 d)和活性稳定期(133～137 d)后可成功启动厌氧氨氧化,活性稳定期NH3-N、 NO2--N、 TN平均去除率分别可达到94.7%、 94.2%、 87.0%。运行至137 d时,生物膜量(VS)、胞外多聚物(EPS)、比厌氧氨氧化活性(SAA)、血红素(Heme)分别达到92.82 mg/g[滤料]、 115.82 mg/g[VS]、 79.68 mg[N]/(g[VS]·d)、 0.93μmol/g[VS], CRI滤池已具有良好的厌氧氨氧化活性。16S rRNA高通量测序结果表明,在厌氧氨氧化活性稳定期,菌群丰度和多样性均随滤料深度的增加而增大,厌氧氨氧化菌属Candidatus Brocadia的相对丰度为1.09%～5.27%,在检出的菌属中占绝对优势,为CRI滤池内发生厌氧氨氧化脱氮提供了基础。
To solve the problem of low TN removal efficiency caused by lack of carbon source in rapid infiltration(CRI) filter,the feasibility and startup performance of anaerobic ammonia oxidation(ANAMMOX) in CRI filter inoculated with aerobic nitrifying sludge were investigated.The results showed that ANAMMOX process could be successfully initiated after adaptation period(1-20 d),activity retardation period(21-49 d),activity enhancement period(50-132 d) and activity stabilization period(133-137 d).The average removal rates of NH3-N,NO2--N and TN could reach 94.7%,94.2% and 87.0% respectively.When running to day 137,the volatile solid(VS),extracellular polymeric substances(EPS),specific ANAMMONX activity(SAA) and heme reached 92.82 mg/g [filter material],115.82 mg/g[VS],79.68 mg[N]/(g[VS]·d),and 0.93 μmol/g[VS] respectively,which indicating that CRI filter had good ANAMMOX activity.16 S rRNA high-throughput sequencing results showed that,the abundance and diversity of microbial community increased with the increase of filter material depth during the stabilization period of ANAMMOX activity.The relative abundance of Candidatus Brocadia was 1.09%-5.27%,which was the absolute predominant bacteria in the detected bacteria and provided basis for ANAMMOX denitrification in CRI filter.
To solve the problem of low TN removal efficiency caused by lack of carbon source in rapid infiltration(CRI) filter,the feasibility and startup performance of anaerobic ammonia oxidation(ANAMMOX) in CRI filter inoculated with aerobic nitrifying sludge were investigated.The results showed that ANAMMOX process could be successfully initiated after adaptation period(1-20 d),activity retardation period(21-49 d),activity enhancement period(50-132 d) and activity stabilization period(133-137 d).The average removal rates of NH3-N,NO2--N and TN could reach 94.7%,94.2% and 87.0% respectively.When running to day 137,the volatile solid(VS),extracellular polymeric substances(EPS),specific ANAMMONX activity(SAA) and heme reached 92.82 mg/g [filter material],115.82 mg/g[VS],79.68 mg[N]/(g[VS]·d),and 0.93 μmol/g[VS] respectively,which indicating that CRI filter had good ANAMMOX activity.16 S rRNA high-throughput sequencing results showed that,the abundance and diversity of microbial community increased with the increase of filter material depth during the stabilization period of ANAMMOX activity.The relative abundance of Candidatus Brocadia was 1.09%-5.27%,which was the absolute predominant bacteria in the detected bacteria and provided basis for ANAMMOX denitrification in CRI filter.
引文
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[16]邹晓凤,洪卫,刘勃,等.焦化废水生化处理有机营养剂增效及菌群结构变化研究[J].工业用水与废水,2017,48(6):22-26.
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[19]汪瑶琪,张敏,姜滢,等.厌氧氨氧化启动过程及微生物群落结构特征[J].环境科学,2017,38(12):5184-5191.
[20]曹雁,王桐屿,秦玉洁,等.厌氧氨氧化反应器脱氮性能及细菌群落多样性分析[J].环境科学,2017,38(4):1544-1550.
[2]陈佼,张建强,许文来,等.人工快速渗滤系统处理含盐生活污水可行性初探[J].水处理技术,2016,42(4):80-83.
[3]骆灵喜,许晓繁,张美娟,等.人工快渗工艺在工业园区污水深度处理中的应用[J].广东化工,2018,45(3):143-145.
[4]蒲小东,魏世清,宋立,等.强化CRI系统布水方式处理猪场废水厌氧消化液[J].中国沼气,2014,32(6):49-50.
[5]刘甜甜,杨培,刘牡,等.盐度负荷冲击对晚期垃圾渗滤液自养脱氮的影响[J].工业用水与废水,2018,49(3):12-16.
[6]MA B,WANG S,CAO S,et al.Biological nitrogen removal from sewage via anammox:Recent advances[J].Bioresource Technology,2016,200:981-990.
[7]于德爽,王晓霞,李津,等.不同污泥源条件下ANAMMOX启动及其与反硝化协同脱氮对比[J].环境工程学报,2013,7(7):2487-2493.
[8]付昆明,苏雪莹,王会芳,等.内回流对厌氧氨氧化UASB反应器脱氮性能的影响[J].中国环境科学,2016,36(12):3560-3566.
[9]张志强,关笑,吕锋,等.悬浮填料对厌氧氨氧化MBR运行的影响特性及机理[J].中国环境科学,2018,38(3):929-934.
[10]DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determining sugars and related substances[J].Analytical Chemistry,2002,28(3):350-356.
[11]SEDMAK J J,GROSSBERG S E.A rapid,sensitive,and versatile assay for protein using Coomassie brilliant blue G250[J].Analytical Biochemistry,1977,79(1-2):544-552.
[12]陈佼.人工快渗系统PN-ANAMMOX耦合脱氮性能及机理研究[D].成都:西南交通大学,2018.
[13]BERRY E A,TRUMPOWER B L.Simultaneous determination of hemes a,b,and c from pyridine hemochrome spectra[J].Analytical Biochemistry,1987,161(1):1-15.
[14]冯莉,于德爽,李津,等.海洋厌氧氨氧化菌的富集培养及其脱氮特性[J].环境科学,2017,38(6):2435-2443.
[15]胡俊,郑江玲,吴越新,等.生物滴滤塔降解甲苯废气长期运行生物膜相特性研究[J].环境科学,2012,33(9):2979-2986.
[16]邹晓凤,洪卫,刘勃,等.焦化废水生化处理有机营养剂增效及菌群结构变化研究[J].工业用水与废水,2017,48(6):22-26.
[17]VIANCELLI A,KUNZ A,ESTEVES P A,et al.Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge[J].Brazilian Archives of Biology&Technology,2011,54(5):1035-1041.
[18]GONZALEZ-MARTINEZ A,OSORIO F,RODRIGUEZ-SANCHEZ A,et al.Bacterial community structure of a lab-scale anammox membrane bioreactor[J].Biotechnology Progress,2015,31(1):186-193.
[19]汪瑶琪,张敏,姜滢,等.厌氧氨氧化启动过程及微生物群落结构特征[J].环境科学,2017,38(12):5184-5191.
[20]曹雁,王桐屿,秦玉洁,等.厌氧氨氧化反应器脱氮性能及细菌群落多样性分析[J].环境科学,2017,38(4):1544-1550.
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