高效液相色谱法检测大米中11种荧光增白剂
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摘要
建立高效液相色谱法检测大米中11种荧光增白剂含量的分析方法。1.000 g样品经1.00 mL 40%乙腈-水溶液(三乙胺调p H值至11.5)提取,氮吹浓缩,经C18柱分离,二极管阵列检测器检测,350 nm波长扫描,外标法定量。高效液相色谱法检测大米中的11种荧光增白剂的检出限均为0.1μg/g,当添加水平为0.5、0.8、1.0μg/mL时,方法回收率为59.3%～89.0%,相对标准偏差(relative standard deviations,RSD)(n=6)在1.1%～5.8%。
A high performance liauid chromatograhy method was established for the determination of 11 kinds of fluorescent whitening agent in rice. The rice was diluted by 40 % acetonitrile-water(adjusted pH to 11.5 by triethylamine),concentrated with nitrogen,separated by C18 column,and determined by photo-diode array at350 nm wavelength with external standard method for quantification. The limits of the detection(LOD)of the11 kinds of fluorescent whitening agent which were detected by high performance liquid chromatography in rice were all 0.1 μg/g. The recoveries of the 11 kinds of fluorescent whitening agent were all between 59.3 %-89.0 %when spiked at 0.5,0.8 and 1.0 μg/mL. The relative standard deviations(RSD)of the method were 1.1 %-5.8 %(n=6).
A high performance liauid chromatograhy method was established for the determination of 11 kinds of fluorescent whitening agent in rice. The rice was diluted by 40 % acetonitrile-water(adjusted pH to 11.5 by triethylamine),concentrated with nitrogen,separated by C18 column,and determined by photo-diode array at350 nm wavelength with external standard method for quantification. The limits of the detection(LOD)of the11 kinds of fluorescent whitening agent which were detected by high performance liquid chromatography in rice were all 0.1 μg/g. The recoveries of the 11 kinds of fluorescent whitening agent were all between 59.3 %-89.0 %when spiked at 0.5,0.8 and 1.0 μg/mL. The relative standard deviations(RSD)of the method were 1.1 %-5.8 %(n=6).
引文
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[12]张建莹,肖锋,叶刚,等.超高效液相色谱-串联质谱法检测食用菌中荧光增白剂[J].食品安全质量检测学报,2014(9):2682-2688
[13]张宪臣,李蓉,周艳萍,等.QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂[J].分析化学,2017,45(5):777-783
[14]李蓉,何春梅,薄艳娜,等.高效液相色谱-三重四极杆质谱法测定面粉中7种荧光增白剂[J].色谱,2017,35(2):162-168
[15]张云,陈泽宇,吕水源,等.高效液相色谱法测定食用油中10种荧光增白剂迁移量[J].食品工业科技,2015,36(24):82-84
[16]张云,陈泽宇,吕水源,等.高效液相色谱法测定食品模拟物中十种荧光增白剂迁移量[J].海峡科技与产业,2015(11):62-65
[2]王景国,容建明.国内外荧光增白剂的状况与展望[J].染料与染色, 2002, 39(1):9-13
[3]董仲生.荧光增白剂的实用性和经济性及其对人与环境的影响[J].中国洗涤用品工业, 2011(5):25-35
[4]唐丽丽,孙彬青,汤佳敏,等.食品用纸包装材料中荧光增白剂的检测与分析[J].包装世界, 2015(6):56-57
[5]黄建勤,肖剑.荧光增白剂在食品及餐饮行业中的应用及检测方法综述[J].食品工业, 2012(7):147-149
[6]Mishra H, Zhang Y, Geddes C D. Metal enhanced fluorescence of the fluorescent brightening agent Tinopal-CBX near silver island film[J]. Dyes&Pigments, 2011, 91(2):225-230
[7]黄蔷,蒋定国,柳洁,等.高效液相色谱法测定食品包装纸中的5种荧光增白剂[J].中国卫生检验杂志, 2015(20):3439-3441
[8]冼燕萍,郭新东,穆同娜,等.食品接触塑料容器中限用荧光增白剂184和393的迁移行为研究[J].质谱学报, 2014, 35(6):530-536
[9]U.S. Food and Drug Administration. Colorants for polymers:Title 21-ChapterⅠ-Subchapter B-Part 178-Subpart D-Section 178.3297[EB/OL]. Code of Federal Regulations,(1983-10-14)[2017-04-01]. http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch. cfmfr=178.3297
[10]中华人民共和国国家卫生和计划生育委员会.GB 9685-2016食品安全国家标准食品接触材料及制品用添加剂使用标准[S].北京:中国标准出版社,2016
[11]Dr Otto G Piringer,Dr Albert L Baner.Community Legislation on Materials and Articles Intended to Come into Contact with Foodstuffs[EB/OL].Plastic Packaging:Interactions with Food and Pharmaceuticals,Second Edition,2002,L 220/18-L 220/58[2008-02-12].https://doi.org/10.1002/9783527621422.ch13
[12]张建莹,肖锋,叶刚,等.超高效液相色谱-串联质谱法检测食用菌中荧光增白剂[J].食品安全质量检测学报,2014(9):2682-2688
[13]张宪臣,李蓉,周艳萍,等.QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂[J].分析化学,2017,45(5):777-783
[14]李蓉,何春梅,薄艳娜,等.高效液相色谱-三重四极杆质谱法测定面粉中7种荧光增白剂[J].色谱,2017,35(2):162-168
[15]张云,陈泽宇,吕水源,等.高效液相色谱法测定食用油中10种荧光增白剂迁移量[J].食品工业科技,2015,36(24):82-84
[16]张云,陈泽宇,吕水源,等.高效液相色谱法测定食品模拟物中十种荧光增白剂迁移量[J].海峡科技与产业,2015(11):62-65