早胜牛微卫星多态性及其与生长性状的关联分析
|
摘要
为了更好地了解早胜牛的遗传信息,为早胜牛的选育保种提供理论依据,本试验选取13对微卫星引物,应用PCR扩增和毛细管电泳的方法对48头早胜牛进行遗传多样性分析,并对13个位点不同基因型与早胜牛的初生重、6月龄重、成年体重、体高、体长、胸围和管围7个性状进行关联分析。结果显示,13个微卫星位点平均等位基因数目为9.54,平均有效等位基因数为4.792,平均观测杂合度为0.498,平均期望杂合度为0.783,平均多态信息含量为0.712。关联分析发现,与初生重存在关联的位点有D12S4和D15S10;与6月龄重存在关联的位点有D18S5和D12S4;与成年体重存在关联的位点有D14S31和D11S15;与体高存在关联的位点有D18S5、D15S10和D19S2;与体长存在关联的位点有D15S10、D14S31和D19S2;与胸围存在关联的位点有D18S5、D12S4和D19S2;与管围存在关联的位点有D18S5和D19S2。研究发现,选取的13个位点中存在关联的位点有6个:D18S5、D12S4、D15S10、D14S31、D19S2和D11S15。本试验所选微卫星位点在早胜牛群体中存在较丰富的多态性,并与经济性状具有关联性,可用于早胜牛遗传资源评价及早期选育改良。
To better understand the genetic information and provide theoretical basis for the breeding and conservation of Zaosheng cattle,13 pairs of microsatellite primers were used to analyze the genetic diversity by PCR amplification and capillary electrophoresis in 48 Zaosheng cattle.The correlation between different genotypes at 13 microsatellite loci and seven traits(birth weight,6-month-old weight,adult weight,body height,body length,chest circumference and cannon circumference) of Zaosheng cattle were analyzed.The results showed that the average number of alleles at 13 microsatellite polymorphic loci was 9.54,the average effective allele was 4.792,the average observed heterozygosity was 0.498,the average expected heterozygosity was 0.783,and the average polymorphic information content was 0.712.The correlation analysis results showed that D12 S4 and D15 S10 were correlated with birth weight;D18 S5 and D12 S4 were correlated with 6-month-old weight;D14 S31 and D11 S15 were correlated with adult weight;D18 S5,D15 S10 and D19 S2 were correlated with body height;D15 S10,D14 S31 and D19 S2 were correlated with body length;D18 S5,D12 S4 and D19 S2 were correlated with chest circumference;D18 S5 and D19 S2 were correlated with cannon circumference.This results found that 6 loci(D18 S5,D12 S4,D15 S10,D14 S31,D19 S2 and D11 S15) were associated with the traits of Zaosheng cattle among the selected 13 microsatellite loci.The microsatellite loci selected in this experiment had rich polymorphisms in Zaosheng cattle population,and were associated with economic traits,which could be used for genetic resources evaluation and early breeding improvement of Zaosheng cattle.
To better understand the genetic information and provide theoretical basis for the breeding and conservation of Zaosheng cattle,13 pairs of microsatellite primers were used to analyze the genetic diversity by PCR amplification and capillary electrophoresis in 48 Zaosheng cattle.The correlation between different genotypes at 13 microsatellite loci and seven traits(birth weight,6-month-old weight,adult weight,body height,body length,chest circumference and cannon circumference) of Zaosheng cattle were analyzed.The results showed that the average number of alleles at 13 microsatellite polymorphic loci was 9.54,the average effective allele was 4.792,the average observed heterozygosity was 0.498,the average expected heterozygosity was 0.783,and the average polymorphic information content was 0.712.The correlation analysis results showed that D12 S4 and D15 S10 were correlated with birth weight;D18 S5 and D12 S4 were correlated with 6-month-old weight;D14 S31 and D11 S15 were correlated with adult weight;D18 S5,D15 S10 and D19 S2 were correlated with body height;D15 S10,D14 S31 and D19 S2 were correlated with body length;D18 S5,D12 S4 and D19 S2 were correlated with chest circumference;D18 S5 and D19 S2 were correlated with cannon circumference.This results found that 6 loci(D18 S5,D12 S4,D15 S10,D14 S31,D19 S2 and D11 S15) were associated with the traits of Zaosheng cattle among the selected 13 microsatellite loci.The microsatellite loci selected in this experiment had rich polymorphisms in Zaosheng cattle population,and were associated with economic traits,which could be used for genetic resources evaluation and early breeding improvement of Zaosheng cattle.
引文
[1] 徐振飞,赫春杰,张有龙,等.庆阳肉牛产业发展现状、存在问题及对策建议[J].中国牛业科学,2015,41(5):80-83.XU Z F,HE C J,ZHANG Y L,et al.The development status,existential problems and countermeasures of beef cattle industry in Qingyang city[J].China Cattle Science,2015,41(5):80-83.(in Chinese)
[2] 张力,朱玲玲,王彩莲,等.早胜牛资源现状及发展建议[J].甘肃农业科技,2017,11:83-86.ZHANG L,ZHU L L,WANG C L,et al.Current situation and development suggestions of Zaosheng cattle resources[J].Gansu Agricultural Science and Technology,2017,11:83-86.(in Chinese)
[3] 徐建峰,容维中,王珂,等.早胜牛种质资源现状调查与分析[J].中国牛业科学,2016,41(2):69-72.XU J F,RONG W Z,WANG K,et al.Investigation and analysis on current situation of germplasm resources in Zaosheng cattle[J].China Cattle Science,2016,41(2):69-72.(in Chinese)
[4] 王晓静,霍生东,陈士恩,等.‘早胜牛’微卫星基因位点遗传多样性分析[J].中国农学通报,2015,31(2):1-7.WANG X J,HUO S D,CHEN S E,et al.The analysis of genetic diversity of microsatellite loci in ‘Zaosheng cattle’[J].Chinese Agricultural Science Bulletin,2015,31(2):1-7.(in Chinese)
[5] 王洪程,昝林森,宋付标,等.秦川牛微卫星基因位点遗传多样性分析[J].中国农学通报,2011,27(11):10-14.WANG H C,ZAN L S,SONG F B,et al.The analysis of genetic diversity of microsatellite loci in Qinchuan cattle[J].Chinese Agricultural Science Bulletin,2011,27(11):10-14.(in Chinese)
[6] 李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答[J].作物学报,2010,36(6):918-931.LI H H,ZHANG L Y,WANG J K,Analysis and answers to frequently asked questions in quantitative trait locus mapping[J].Acta Agronomica Sinica,2010,36(6):918-931.(in Chinese)
[7] TóTH G,GáSPáRI Z,JURKA J.Microsatellites in different eukaryotic genomes:Survey and analysis[J].Genome Research,2000,10(7):967-981.
[8] SRINIVASA J,ARUN B,MISHRA V K,et al.Zinc and iron concentration QTL mapped in a Triticum spelta×T.aestivum[J].Theoretical and Applied Genetics,2014,127(7):1643-1651.
[9] GOLDSTEIN D B,LINARES A R,CAVALLI-SFORZA L L,et al.An evaluation of genetic distances for use with microsatellite loci[J].Genetics,1995,139(1):463-471.
[10] NAPOLITANO F,LEONE P,PUPPO S,et al.Exploitation of microsatellites as genetic markers of beef-performance traits in Piemontese×Chianina crossbred cattle[J].Journal of Animal Breeding & Genetics,1996,113(1-6):157-162.
[11] 曹红鹤,王雅春,陈幼春,等.南阳、皮埃蒙特及其杂交牛的血液生化遗传标记与生长性状关系[J].畜牧兽医学报,1999,30(6):496-503.CAO H H,WANG Y C,CHEN Y C,et al.Association of biochemical genetic marker with performance traits in Nanyang,Piemontese and their hybrid[J].Acta Veterinaria et Zooteachnica Sinica,1999,30(6):496-503.(in Chinese)
[12] BARON B,POIRIER C,SIMONCHAZOTTES D,et al.A new strategy useful for rapid identification of microsatellites from DNA libraries with large size inserts[J].Nucleic Acids Research,1992,20(14):3665-3669.
[13] 赵宗胜,郭继刚,王遵宝.7个微卫星标记与中国荷斯坦奶牛产奶性状的关联性研究[J].黑龙江畜牧兽医,2010,10:19-22.ZHAO Z S,GUO J G,WANG Z B.The correlated research between seven microsatellite markers and latation trait of Holstein[J].Heilongjiang Animal Science and Veterinary Medicine,2010,10:19-22.(in Chinese)
[14] 成述儒,王华,樊庆山,等.甘肃高山细毛羊DRB1基因内含子2微卫星多态性与生长速度的关联分析[J].浙江农业学报,2016,28(12):2021-2027.CHENG S R,WANG H,FAN Q S,et al.Correlation analysis of DRB1 gene intron 2 microsatellite polymorphism and growth rate on Gansu Alpine Merino[J].Acte Agriculturae Zhejiangensis,2016,28(12):2021-2027.(in Chinese)
[15] 张子军,凌英会,任春环,等.甘肃5个肉用绵羊品种的微卫星遗传多样性及其与生产性能的关联性分析[J].西北农林科技大学学报(自然科学版),2013,41(8):31-37.ZHANG Z J,LING Y H,REN C H,et al.Microsatellite markers genetic diversity and growth traits correlation of five meat sheep breeds in Gansu[J].Journal of Northwest A & F University(Natural Science Edition),2013,41(8):31-37.(in Chinese)
[16] ARRANZE J J,BAYóN Y,SAN P F.Comparison of protein markers and microsatellites in differentiation of cattle populations[J].Animal Genetics,2010,27(6):415-419.
[17] YANG Y,LIU N,ZUO Q,et al.The screening of microsatellite DNA loci for the identification of individual Tibetan foxes,using copro-DNA samples[J].Acta Theriologica Sinica,2014,34(4):387-392.
[18] 王斌,昝林森,杜宝民,等.西镇牛微卫星DNA与生长发育性状的关联性分析[J].西北农林科技大学学报(自然科学版),2017,45(3):7-16.WANG B,ZAN L S,DU B M,et al.Association of microsatellite DNA with growth traits of Xizhen cattle[J].Journal of Northwest A & F University(Natural Science Edition),2017,45(3):7-16.(in Chinese)
[19] 赵梦然,陈强,张金霞,等.IGS2-RFLP、SCoT和ISSR在白灵侧耳遗传多样性分析中的比较研究[J].菌物学报,2013,32(4):682-689.ZHAO M R,CHEN Q,ZHANG J X,et al.Comparison studies of genetic diversity of Pleurous eryngii var.tuoliensis by IGS2-RFLP,SCoT and ISSR markers[J].Mycosystema,2013,32(4):682-689.(in Chinese)
[20] 汪桂玲,袁一鸣,李家乐.中国五大湖三角帆蚌群体遗传多样性及亲缘关系的SSR分析[J].水产学报,2007,31(2):152-158.WANG G L,YUAN Y M,LI J L.SSR analysis of genetic diversity and phylogenetic relationships among different populations of Hyriopsis cumingii from the five lakes of China[J].Journal of Fisheries of China,2007,31(2):152-158.(in Chinese)
[21] 陈腾,张琳琳,赖江华,等.中国东乡族9个STR基因座遗传多态性研究[J].遗传,2002,24(6):247-250.CHEN T,ZHANG L L,LAI J H,et al.Genetic polymorphisms of 9 STR loci in Dongxiang ethnic group of China[J].Hereditas,2002,24(6):247-250.(in Chinese)
[22] 韦树谷,曾华兰,叶鹏盛,等.茄子品种抗黄萎病性状与SRAP标记的关联性分析[J].西南农业学报,2015,28(6):2511-2515.WEI S G,ZENG H L,YE P S,et al.Association analysis of verticillium wilt resistant traits with SRAP markers in eggplant varieties[J].Southwest China Jounal of Agricultural Sciences,2015,28(6):2511-2515.(in Chinese)
[23] 严林俊,陈宏,房兴堂,等.中国荷斯坦牛微卫星基因座遗传多态性研究[J].西北农林科技大学学报(自然科学版),2007,35(7):23-27.YAN L J,CHEN H,FANG X T,et al.Polymorphism distributions of microsatellite loci in Chinese Holstein cattle[J].Journal of Northwest A & F University(Natural Science Edition),2007,35(7):23-27.(in Chinese)
[24] 王遵宝,赵宗胜,吴洪宾,等.绵羊皮肤源EST-SSR标记与羊毛性状的关联性分析[J].农业生物技术学报,2011,19(6):1056-1062.WANG Z B,ZHAO Z S,WU H B,et al.Correlation analysis of ovine skin derived EST-SSR markers with wool traits[J].Journal of Agricultural Biotechnology,2011,19(6):1056-1062.(in Chinese)
[25] 王志新,梁海鹰,杜晓东,等.马氏珠母贝热休克蛋白HSP60基因的克隆与表达分析[J].广东海洋大学学报,2013,33(6):14-23.WANG Z X,LIANG H Y,DU X D,et al.Cloning and experess characters of HSP60 gene from Pinctada martensii[J].Journal of Guangdong Ocean University,2013,33(6):14-23.(in Chinese)
[26] 王斌,昝林森,杜宝民,等.宣汉牛微卫星DNA与生长发育性状的关联分析[J].农业生物技术学报,2018,26(7):1174-1185.WANG B,ZAN L S,DU B M,et al.Association analysis of microsatellite DNA with growth and development traits of Xuanhan cattle (Bos taurus)[J].Journal of Agricultural Biotechnology,2018,26(7):1174-1185.(in Chinese)
[27] 汪聪勇,高腾云,祁兴磊,等.夏南牛体尺性状与微卫星DNA的相关分析[J].西南大学学报(自然科学版),2010,32(2):44-51.WANG C Y,GAO T Y,QI X L,et al.Correlation analysis of microsatellite DNA marker and body size traits in Xianan cattle[J].Journal of Southwest University (Natural Science Edition),2010,32(2):44-51.
[2] 张力,朱玲玲,王彩莲,等.早胜牛资源现状及发展建议[J].甘肃农业科技,2017,11:83-86.ZHANG L,ZHU L L,WANG C L,et al.Current situation and development suggestions of Zaosheng cattle resources[J].Gansu Agricultural Science and Technology,2017,11:83-86.(in Chinese)
[3] 徐建峰,容维中,王珂,等.早胜牛种质资源现状调查与分析[J].中国牛业科学,2016,41(2):69-72.XU J F,RONG W Z,WANG K,et al.Investigation and analysis on current situation of germplasm resources in Zaosheng cattle[J].China Cattle Science,2016,41(2):69-72.(in Chinese)
[4] 王晓静,霍生东,陈士恩,等.‘早胜牛’微卫星基因位点遗传多样性分析[J].中国农学通报,2015,31(2):1-7.WANG X J,HUO S D,CHEN S E,et al.The analysis of genetic diversity of microsatellite loci in ‘Zaosheng cattle’[J].Chinese Agricultural Science Bulletin,2015,31(2):1-7.(in Chinese)
[5] 王洪程,昝林森,宋付标,等.秦川牛微卫星基因位点遗传多样性分析[J].中国农学通报,2011,27(11):10-14.WANG H C,ZAN L S,SONG F B,et al.The analysis of genetic diversity of microsatellite loci in Qinchuan cattle[J].Chinese Agricultural Science Bulletin,2011,27(11):10-14.(in Chinese)
[6] 李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答[J].作物学报,2010,36(6):918-931.LI H H,ZHANG L Y,WANG J K,Analysis and answers to frequently asked questions in quantitative trait locus mapping[J].Acta Agronomica Sinica,2010,36(6):918-931.(in Chinese)
[7] TóTH G,GáSPáRI Z,JURKA J.Microsatellites in different eukaryotic genomes:Survey and analysis[J].Genome Research,2000,10(7):967-981.
[8] SRINIVASA J,ARUN B,MISHRA V K,et al.Zinc and iron concentration QTL mapped in a Triticum spelta×T.aestivum[J].Theoretical and Applied Genetics,2014,127(7):1643-1651.
[9] GOLDSTEIN D B,LINARES A R,CAVALLI-SFORZA L L,et al.An evaluation of genetic distances for use with microsatellite loci[J].Genetics,1995,139(1):463-471.
[10] NAPOLITANO F,LEONE P,PUPPO S,et al.Exploitation of microsatellites as genetic markers of beef-performance traits in Piemontese×Chianina crossbred cattle[J].Journal of Animal Breeding & Genetics,1996,113(1-6):157-162.
[11] 曹红鹤,王雅春,陈幼春,等.南阳、皮埃蒙特及其杂交牛的血液生化遗传标记与生长性状关系[J].畜牧兽医学报,1999,30(6):496-503.CAO H H,WANG Y C,CHEN Y C,et al.Association of biochemical genetic marker with performance traits in Nanyang,Piemontese and their hybrid[J].Acta Veterinaria et Zooteachnica Sinica,1999,30(6):496-503.(in Chinese)
[12] BARON B,POIRIER C,SIMONCHAZOTTES D,et al.A new strategy useful for rapid identification of microsatellites from DNA libraries with large size inserts[J].Nucleic Acids Research,1992,20(14):3665-3669.
[13] 赵宗胜,郭继刚,王遵宝.7个微卫星标记与中国荷斯坦奶牛产奶性状的关联性研究[J].黑龙江畜牧兽医,2010,10:19-22.ZHAO Z S,GUO J G,WANG Z B.The correlated research between seven microsatellite markers and latation trait of Holstein[J].Heilongjiang Animal Science and Veterinary Medicine,2010,10:19-22.(in Chinese)
[14] 成述儒,王华,樊庆山,等.甘肃高山细毛羊DRB1基因内含子2微卫星多态性与生长速度的关联分析[J].浙江农业学报,2016,28(12):2021-2027.CHENG S R,WANG H,FAN Q S,et al.Correlation analysis of DRB1 gene intron 2 microsatellite polymorphism and growth rate on Gansu Alpine Merino[J].Acte Agriculturae Zhejiangensis,2016,28(12):2021-2027.(in Chinese)
[15] 张子军,凌英会,任春环,等.甘肃5个肉用绵羊品种的微卫星遗传多样性及其与生产性能的关联性分析[J].西北农林科技大学学报(自然科学版),2013,41(8):31-37.ZHANG Z J,LING Y H,REN C H,et al.Microsatellite markers genetic diversity and growth traits correlation of five meat sheep breeds in Gansu[J].Journal of Northwest A & F University(Natural Science Edition),2013,41(8):31-37.(in Chinese)
[16] ARRANZE J J,BAYóN Y,SAN P F.Comparison of protein markers and microsatellites in differentiation of cattle populations[J].Animal Genetics,2010,27(6):415-419.
[17] YANG Y,LIU N,ZUO Q,et al.The screening of microsatellite DNA loci for the identification of individual Tibetan foxes,using copro-DNA samples[J].Acta Theriologica Sinica,2014,34(4):387-392.
[18] 王斌,昝林森,杜宝民,等.西镇牛微卫星DNA与生长发育性状的关联性分析[J].西北农林科技大学学报(自然科学版),2017,45(3):7-16.WANG B,ZAN L S,DU B M,et al.Association of microsatellite DNA with growth traits of Xizhen cattle[J].Journal of Northwest A & F University(Natural Science Edition),2017,45(3):7-16.(in Chinese)
[19] 赵梦然,陈强,张金霞,等.IGS2-RFLP、SCoT和ISSR在白灵侧耳遗传多样性分析中的比较研究[J].菌物学报,2013,32(4):682-689.ZHAO M R,CHEN Q,ZHANG J X,et al.Comparison studies of genetic diversity of Pleurous eryngii var.tuoliensis by IGS2-RFLP,SCoT and ISSR markers[J].Mycosystema,2013,32(4):682-689.(in Chinese)
[20] 汪桂玲,袁一鸣,李家乐.中国五大湖三角帆蚌群体遗传多样性及亲缘关系的SSR分析[J].水产学报,2007,31(2):152-158.WANG G L,YUAN Y M,LI J L.SSR analysis of genetic diversity and phylogenetic relationships among different populations of Hyriopsis cumingii from the five lakes of China[J].Journal of Fisheries of China,2007,31(2):152-158.(in Chinese)
[21] 陈腾,张琳琳,赖江华,等.中国东乡族9个STR基因座遗传多态性研究[J].遗传,2002,24(6):247-250.CHEN T,ZHANG L L,LAI J H,et al.Genetic polymorphisms of 9 STR loci in Dongxiang ethnic group of China[J].Hereditas,2002,24(6):247-250.(in Chinese)
[22] 韦树谷,曾华兰,叶鹏盛,等.茄子品种抗黄萎病性状与SRAP标记的关联性分析[J].西南农业学报,2015,28(6):2511-2515.WEI S G,ZENG H L,YE P S,et al.Association analysis of verticillium wilt resistant traits with SRAP markers in eggplant varieties[J].Southwest China Jounal of Agricultural Sciences,2015,28(6):2511-2515.(in Chinese)
[23] 严林俊,陈宏,房兴堂,等.中国荷斯坦牛微卫星基因座遗传多态性研究[J].西北农林科技大学学报(自然科学版),2007,35(7):23-27.YAN L J,CHEN H,FANG X T,et al.Polymorphism distributions of microsatellite loci in Chinese Holstein cattle[J].Journal of Northwest A & F University(Natural Science Edition),2007,35(7):23-27.(in Chinese)
[24] 王遵宝,赵宗胜,吴洪宾,等.绵羊皮肤源EST-SSR标记与羊毛性状的关联性分析[J].农业生物技术学报,2011,19(6):1056-1062.WANG Z B,ZHAO Z S,WU H B,et al.Correlation analysis of ovine skin derived EST-SSR markers with wool traits[J].Journal of Agricultural Biotechnology,2011,19(6):1056-1062.(in Chinese)
[25] 王志新,梁海鹰,杜晓东,等.马氏珠母贝热休克蛋白HSP60基因的克隆与表达分析[J].广东海洋大学学报,2013,33(6):14-23.WANG Z X,LIANG H Y,DU X D,et al.Cloning and experess characters of HSP60 gene from Pinctada martensii[J].Journal of Guangdong Ocean University,2013,33(6):14-23.(in Chinese)
[26] 王斌,昝林森,杜宝民,等.宣汉牛微卫星DNA与生长发育性状的关联分析[J].农业生物技术学报,2018,26(7):1174-1185.WANG B,ZAN L S,DU B M,et al.Association analysis of microsatellite DNA with growth and development traits of Xuanhan cattle (Bos taurus)[J].Journal of Agricultural Biotechnology,2018,26(7):1174-1185.(in Chinese)
[27] 汪聪勇,高腾云,祁兴磊,等.夏南牛体尺性状与微卫星DNA的相关分析[J].西南大学学报(自然科学版),2010,32(2):44-51.WANG C Y,GAO T Y,QI X L,et al.Correlation analysis of microsatellite DNA marker and body size traits in Xianan cattle[J].Journal of Southwest University (Natural Science Edition),2010,32(2):44-51.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |