蓖麻LRR-RKsⅡ家族和LRR-RKsⅩ家族的生物信息学分析
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摘要
本研究基于对蓖麻油菜素内酯信号转导途径中起主要作用的受体BRI1和共受体BAK1所在的2个蛋白家族LRR-RKsⅡ家族和LRR-RKsⅩ家族成员进行生物信息学分析,主要包括:对受体BRI1和BAK1进行蛋白家族成员的鉴定.结构域分析结果表明,这2个家族的蛋白均含有2种起主要作用的结构域,分别为LRR x和Pkinase.受体激酶BRI1所具有的结构域LRR 8和Pkinase可能与蓖麻种子萌发和叶肉细胞伸长、分裂和分化有关,植物体内缺失BRI1导致植株对BR敏感度下降;受体激酶BAK1具有的LRR 2和Pkinase结构域可能与蓖麻胚乳发育和叶子发育有关.系统发育树显示由同一祖先进化而来的具有相同进化距离的氨基酸序列未必会进化为同一种蛋白,且这可能与特定的进化距离相关.这2个家族在蓖麻Ⅱ/Ⅲ期和Ⅴ/Ⅵ期发育阶段的胚乳、叶子、雄花以及萌发的种子这5种组织中的表达量图谱分析均显示,除在蓖麻胚乳Ⅴ/Ⅵ期发育阶段的组织外,在其他组织中表达水平都较高.
Bioinformatics analysis was used to study members of LRR-RKsⅡ and LRR-RKsⅩ families,which play a major role in the signal transduction pathway of Brassinosteroids,including identification of members of the protein family of receptors BRI1 and BAK1. The domain analysis showed that the proteins of these two families both contain two main domains,LRR x and Pkinase. The domains LRR 8 and Pkinase with receptor kinase BRI1 may be related to castor seed germination and elongation,division and differentiation of mesophyll cells,and the absence of BRI1 in plants may lead to decreased sensitivity to BR. The LRR 2 and Pinase domains of receptor kinase BAK1 may be related to castor endosperm development and leaf development. Phylogenetic trees show that amino acid sequences with the same evolutionary distance evolved from the same ancestor may not necessarily evolve into the same protein,and this may be related to a specific evolutionary distance. Analysis of the expression profiles of the two families in the endosperm,leaves,male flowers and germinating seeds of Castor Ⅱ/Ⅲ and Ⅴ/Ⅵ stages shows that the expression levels are higher in other tissues except those in Castor Ⅴ/Ⅵ stages.
Bioinformatics analysis was used to study members of LRR-RKsⅡ and LRR-RKsⅩ families,which play a major role in the signal transduction pathway of Brassinosteroids,including identification of members of the protein family of receptors BRI1 and BAK1. The domain analysis showed that the proteins of these two families both contain two main domains,LRR x and Pkinase. The domains LRR 8 and Pkinase with receptor kinase BRI1 may be related to castor seed germination and elongation,division and differentiation of mesophyll cells,and the absence of BRI1 in plants may lead to decreased sensitivity to BR. The LRR 2 and Pinase domains of receptor kinase BAK1 may be related to castor endosperm development and leaf development. Phylogenetic trees show that amino acid sequences with the same evolutionary distance evolved from the same ancestor may not necessarily evolve into the same protein,and this may be related to a specific evolutionary distance. Analysis of the expression profiles of the two families in the endosperm,leaves,male flowers and germinating seeds of Castor Ⅱ/Ⅲ and Ⅴ/Ⅵ stages shows that the expression levels are higher in other tissues except those in Castor Ⅴ/Ⅵ stages.
引文
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[22]钱文静,刘静,宋丹,等.水稻油菜素内酯相关基因及其功能研究进展[J].河南农业科学,2013,42(9):1-5.
[2]Baldwin B S,Cossar R D.Castor yield in response to planting date at four locations in the south-central United States[J].Industrial Crops and Products,2009,29(2-3):316-319.
[3]任春环,黄桠锋,郭晓飞,等.蓖麻粕的脱毒及其在畜禽生产中的应用[J].中国饲料,2014(16):36-38,41.
[4]刘鹏.蓖麻细胞色素P450基因RNAi植物表达载体构建及遗传转化的研究[D].沈阳:沈阳农业大学,2012.
[5]熊书.油菜素内酯的生物合成及信号转导研究进展[J].教育教学论坛,2015(19):111-112.
[6]Wanyan Xi,Hao Yu.MOTHER OF FT AND TFL1 regulates seed germination and fertility relevant to the brassinosteroid signaling pathway[J].Plant Signaling&Behavior,2010,5(10):1315-1317.
[7]杨鑫鑫,潘秋红.油菜素甾醇分析方法概述及其在果实中的应用[J].热带生物学报,2016,7(1):139-146.
[8]晋育丹,戴绍军.蛋白质磷酸化在油菜素内酯信号通路中的作用[J].现代农业科技,2014(6):222-223.
[9]卫卓赟,黎家.受体激酶介导的油菜素内酯信号转导途径[J].生命科学,2011,23(11):1106-1113.
[10]田荣,杨勇,王晓峰.植物受体激酶BAK1研究进展[J].西北植物学报,2014,34(3):636-644.
[11]宋婷,熊炜,李莹莹,等.一个水稻富亮氨酸重复类受体蛋白激酶的RNAi及表达分析[J].天津师范大学学报(自然科学版),2016,36(1):48-51.
[12]徐宗昌,王萌,孔英珍.油菜素内酯参与初生细胞壁代谢研究进展[J].安徽农业科学,2016,44(32):1-5,8.
[13]Agnes P Chan,Jonathan Crabtree,Qi Zhao,et al.Draft genome sequence of the ricin-producing oilseed castor bean[J].Nature Biotechnology,2010,28(9):951-956.
[14]Brown A P,Kroon J T M,Swarbreck D,et al.Tissue-Specific Whole Transcriptome Sequencing in Castor,Directed at Understanding Triacylglycerol Lipid Biosynthetic Pathways[J].PLOS ONE,2012,7(2):e30100.
[15]Changlong Wen,Qing Cheng,Liqun Zhao,et al.Identification and characterisation of Dof transcription factors in the cucumber genome[J].Scientific RepoRts,2016,6(1):23072.
[16]于洋,李红岩,张士璀.Maf家族基因的系统演化分析[J].鲁东大学学报(自然科学版),2014,30(4):330-335.
[17]邵慧,冉从福,余静,等.小麦地方品种半截芒Glu-B1位点HMW-GS基因的克隆及序列分析[J].西北农业学报,2015,24(6):21-26.
[18]Steven D Clouse.Brassinosteroid Signal Transduction:From Receptor Kinase Activation to Transcriptional Networks Regulating Plant Development[J].The Plant Cell,2011,23(4):1219-1230.
[19]孙彩霞,李明,乔瑞敏,等.猪EGFL6基因生物信息学分析[J].东北农业大学学报,2016,47(4):16-23.
[20]Jinyeong Cheon,Shozo Fujioka,Brian P Dilkes,et al.Brassinosteroids Regulate Plant Growth through Distinct Signaling Pathways in Selaginella and Arabido-psis[J].PLOS ONE,2013,8(12):e81938.
[21]阳治国,谢甜,王浩杰,等.甘蓝型油菜BnBRI1基因过量表达载体和RNA干扰载体的构建及遗传转化[J].四川大学学报(自然科学版),2016,53(3):689-694.
[22]钱文静,刘静,宋丹,等.水稻油菜素内酯相关基因及其功能研究进展[J].河南农业科学,2013,42(9):1-5.
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