HPLC法测定木瓜中齐墩果酸和熊果酸的含量

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英文篇名：Determination of oleanolic acid and ursolic acid in Chaenomelis Fructus by HPLC 作者：代琪 ; 罗霄 ; 叶俏波 英文作者：Dai Qi;Luo Xiao;Ye Qiaobo;Chengdu Institute of Food and Drug Inspection;Department of Formulas, Chengdu University of Traditional Chinese Medicine; 关键词：木瓜 ; 齐墩果酸 ; 熊果酸 ; HPLC 英文关键词：Chaenomelis Fructus;;Oleanolic Acid;;Ursolic Acid;;HPLC 中文刊名：YTCT 英文刊名：Asia-Pacific Traditional Medicine 机构：成都市食品药品检验研究院;成都中医药大学方剂学教研室; 出版日期：2019-06-05 出版单位：亚太传统医药 年：2019 期：v.15;No.214 语种：中文; 页：YTCT201906027 页数：5 CN：06 ISSN：42-1727/R 分类号：87-91

摘要

目的:使用《中国药典》2015版第一部木瓜含量测定项下方法,测定齐墩果酸和熊果酸时发现存在齐墩果酸和熊果酸的分离效果不佳,流动相在210 nm波长下测定基线有波动,会对峰面积造成影响等问题,故重新建立木瓜中齐墩果酸、熊果酸的含量测定方法。方法:色谱柱为C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%氨水溶液(用磷酸调节pH=7.5)(70∶30);体积流量1.0 mL/min;检测波长210 nm。结果:齐墩果酸和熊果酸对照品均在一定浓度范围内与峰面积呈良好的线性关系;齐墩果酸和熊果酸的加样回收率分别为101.76%、103.02%,RSD分别为1.67%、1.57%。结论:该方法简便、准确、重现性好,齐墩果酸和熊果酸色谱峰分离度在2.0以上,可用于测定木瓜中齐墩果酸和熊果酸的含量。
        Objective:The separation effect of oleanolic acid and ursolic acid was poor when oleanolic acid and ursolic acid were determined by the method of Chaenomelis Fructus content determination in the first volumn of Chinese Pharmacopoeia 2015. The fluctuant baseline of the mobile phase at 210 nm will affect the peak area. Therefore, a new method for determination of oleanolic acid and ursolic acid in Chaenomelis Fructus was established. Methods:The chromatographic column was C_(18)(200 mm×4.6 mm, 5 μm),and the mobile phase was acetonitrile-0.1% a mmonia solution(pH was adjusted to 7.5 with phosphoric acid)(70: 30), volume flow rate was 1.0 mL/min,detection wavelength was 210 nm. Results: Both oleanolic acid and ursolic acid had good linear relationship with peak area within a certain concentration range. The recoveries of oleanolic acid and ursolic acid were 101.76% and 103.02%. RSD was 1.67% and 1.57%. Conclusion:The method is simple, accurate and reproducible. The chromatographic peaks of oleanolic acid and ursolic acid are above 2.0. It can be used for the determination of oleanolic acid and ursolic acid in Chaenomelis Fructus.

引文

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