含血管内皮生长因子缓释微粒的合成与鉴定
摘要
目的:合成含血管内皮生长因子缓释微粒,并检测其体外缓释作用。方法:本实验使用可降解高分子聚乳酸/乙醇酸共聚物,采用乳化分散法,对血管内皮生长因子进行包裹,制成可控制释放的微粒。测定VEGF体外释放情况并描绘成曲线。结果:制得VEGF的缓释微粒大小约80～120nm。体外释放检测开始时为快速释放期,随后为缓慢持续释放。结论:此实验证实成功构建含血管内皮生长因子缓释微粒。
引文
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[2] Park K. Delivery of definable numbers of PLGA microparticles within embryoid bodies[J]. Control Release,2013,168(1):103.
[3] Herrán E, Ruiz-Ortega JA, Aristieta A, et al. In vivo administration of VEGF-and GDNF-releasing biodegradable polymeric microspheres in a severe lesion model of Parkinson's disease[J]. Eur J Phart Biopharm,2013,85(3 Pt B):1183-11190.
[4] Simón-Yarza T, Tamayo E, Benavides C, et al. Functional benefits of PLGA particulates carrying VEGF and CoQ1O in an animal of myocardial ischemia[J]. Int J Pharm,2013,454(2):784-790.
[5] White LJ, Kirby GT, Cox HC, et al. Accelerating protein release from microparticles for regenerative medicine applications[J]. Mater Sci Eng C Mater Biol Appl,2013,33(5):2578-2583.