40份糜子资源淀粉组成及含量分析
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摘要
利用双波长比色法在606 nm处测定直链淀粉的波长,参比波长为480 nm;在538 nm处测定支链淀粉的波长,参比波长为708 nm,测定来源于不同地区的40份糜子淀粉的组成及含量,为优质糜子品种选育提供理论依据。结果发现,23份糜子材料为糯性,17份为粳性;总淀粉含量介于58.43%～75.45%,直链淀粉含量介于0～20.34%,支链淀粉含量介于46.70%～69.61%;各地区糜子直链淀粉含量差异显著,而淀粉含量差异不显著。
The wavelength of amylose was measured at 606 nm by dual-wavelength colorimetry, the reference wavelength was 480 nm. The wavelength of amylopectin was measured at 538 nm, the reference wavelength was 708 nm, and the composition and content of starch on 40 common millet resources originated from different region were determined. The results showed that among the 40 common millet resources, 23 and 17 were waxy and non-waxy, respectively. The total starch content of all resources varied from 58.43% to 75.45%. The content of amylose and amylopectin ranged from 0-20.34% and 46.70%-69.61%, respectively. The difference of amylose content of all materials among in different regions was significant, while there was almost no difference in starch content.
The wavelength of amylose was measured at 606 nm by dual-wavelength colorimetry, the reference wavelength was 480 nm. The wavelength of amylopectin was measured at 538 nm, the reference wavelength was 708 nm, and the composition and content of starch on 40 common millet resources originated from different region were determined. The results showed that among the 40 common millet resources, 23 and 17 were waxy and non-waxy, respectively. The total starch content of all resources varied from 58.43% to 75.45%. The content of amylose and amylopectin ranged from 0-20.34% and 46.70%-69.61%, respectively. The difference of amylose content of all materials among in different regions was significant, while there was almost no difference in starch content.
引文
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[2]HUNT H V,RUDZINSKI A,JIANG H E,et al.Genetic evidence for a western Chinese origin of broomcorn millet(Panicum miliaceum)[J].The Holocene,2018,28(12):1968-1978.
[3]王星玉,王纶.黍稷种质资源描述规范和黍稷标准[M].北京:中国农业出版社,2006:5-15.
[4]王璐琳,王瑞云,何杰丽,等.糜子特异性SSR标记的开发[J].山西农业科学,2018,46(1):1-4,86.
[5]国家谷子糜子产业技术体系.中国国现代农业产业可持续发展战略研究[M].北京:中国农业出版社,2018:25-30.
[6]WANG R Y,HUNT H V,QIAO Z J,et al.Diversity and cultivation of broomcorn millet(Panicum miliaceum L.)in China:a review[J].Economic Botany,2016,70(3):332-342.
[7]WANG R Y,WANG H G,LIU X H,et al.Waxy allelic diversity in common millet(Panicum miliaceum L.)in China[J].The Crop Journal,2018,6(4):377-385.
[8]薛延桃,陆平,乔治军,等.基于SSR标记的黍稷种质资源遗传多样性及亲缘关系研究[J].中国农业科学,2018,51(15):19-39.
[9]乔治军.糜子产业发展现状与思路[J].作物杂志,2013(5):25-27.
[10]王瑞云.糜子遗传多样性及进化研究进展[M].北京:中国农业出版社,2017:10-117.
[11]申瑞玲,马玉玲,杜文娟,等.糜子的营养生理功能特性及产品开发现状[J].粮食与油脂,2017,30(1):9-12.
[12]马清兰,吴茂江.糜子黄酒的制取[J].现代农业,2001(11):30.
[13]田翔,乔治军,秦慧彬,等.比色法测定糜子中的直链淀粉[J].安徽农业科学,2015,43(9):283-284.
[14]王颖,晁桂梅,杨秋歌,等.粳性糜子淀粉与小米淀粉理化性质的比较[J].福建农林大学学报(自然科学版),2014,43(5):523-529.
[15]张丽珍,冀佩双,罗旭枭,等.糜黍淀粉理化性质及消化特性[J].食品科学,2016,37(19):76-81.
[16]CHAO G M,GAO J F,LIU R,et al.Starch physicochemical properties of waxy proso millet(Panicum miliaceum L.)[J].Starch/St覿rke,2014,66:1005-1012.
[17]胡博文,谷娇娇,贾琰,等.盐胁迫对寒地粳稻籽粒淀粉形成积累及产量的影响[J].华北农学报,2019,34(1):115-123.
[18]张善磊,岳红亮,赵春芳,等.粳稻栽培品种中淀粉合成相关基因的等位变异及群体结构分析[J].华北农学报,2018,33(6):116-122.
[19]姚亚平,田呈瑞,张国权,等.糜子淀粉理化性质的分析[J].中国粮油学报,2009,24(9):45-52.
[20]柴岩.糜子[M].北京:中国农业出版社,1999:162.
[21]王佳.不同稻米加工成脱水方便米饭的适应性及工艺优化[D].长沙:中南林业科技大学,2012.
[22]文君.玉米直链淀粉的酶法制备及其在软糖中的应用研究[D].武汉:华中农业大学,2012.
[23]JEONG H Y,LIM S T.Crystallinity and pasting properties of freeze-thawed high amylose maize starch[J].Starch/St覿rke,2003,55:511-513.
[24]刘襄河,郑丽璇,郑丽勉,等.双波长法测定常用淀粉原料中直链淀粉、支链淀粉及总淀粉含量[J].广东农业科学,2013(18):97-100.
[25]刘芸,王小平.大米直链淀粉含量测定法的应用与分析[J].粮食加工,2018,43(3):28-30.
[26]谭亮,董琦,耿丹丹,等.双波长比色法测定不同产地和品种青稞中直链淀粉和支链淀粉的含量[J].食品工业科技,2015,36(2):79-84.
[27]邵春甫,李长文,王珊,等.近红外光谱应用于高粱中总淀粉、直链淀粉与支链淀粉的定量分析[J].粮油食品科技,2016,24(2):60-64.
[28]党允卓,吕庆云,周坚.糯米制品专用米研究进展[J].粮食与饲料工业,2018(6):23-27.
[29]田翔,乔治军,陈艳霞.双波长比色法测定糜子中直链/支链淀粉含量[J].中国农学报,2015,31(32):76-80.
[30]王瑞云,刘晓欢,申鸽子,等.5个糜子品种的形态指标差异分析[J].山西农业科学,2013,41(8):1167-1169.
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