Box-Behnken响应面法结合多指标综合评分法优选柴胡安心胶囊的水提工艺
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摘要
目的:优化柴胡安心胶囊的水提工艺。方法:以提取液中没食子酸、绿原酸、葛根素、大豆苷、芦丁、肉桂酸、槲皮素的含量和出膏率的综合评分值为考察指标,以加液倍数、浸泡时间、回流时间、提取次数为因素,在单因素试验基础上运用Box-Behnken响应面法对柴胡安心胶囊的水提工艺进行优化并进行验证试验。结果:最优提取工艺为加11倍水、浸泡10 h,提取2次,每次回流1.5 h;验证试验中3批样品提取后的综合评分值(91.37%)与预测值(91.38%)相比较,相对误差为1.87%(RSD<2%,n=3)。结论:优选的提取工艺方法简单、结果稳定,可用于后续柴胡安心胶囊的制剂生产。
OBJECTIVE: To optimize the water extraction technology of Chaihu anxin capsules. METHODS: Taking comprehensive scoring value of the contents of gallic acid,chlorogenic acid,puerarin,glycoside,rutin,cinnamic acid,quercetin and the yield of extract as investigation index,using multiple of adding liquid,soaking time,reflux time and extraction times as factors,water extraction technology of Chaihu anxin capsule was optimized by Box-Behnken response surface method based on single factor test. Validation test was conducted. RESULTS:The optimal extraction technology of Chaihu anxin capsules was adding 11 times of water,soaking for 10 h,extracting for 2 times,refluxing for 1.5 h each time. In validation test,the relative deviation of comprehensive scoring value to predicted value was 1.87% for 3 batches of samples(RSD<2%, n=3).CONCLUSIONS:The optimal extraction technology is simple,stable and suitable for further production of Chaihu anxin capsules.
OBJECTIVE: To optimize the water extraction technology of Chaihu anxin capsules. METHODS: Taking comprehensive scoring value of the contents of gallic acid,chlorogenic acid,puerarin,glycoside,rutin,cinnamic acid,quercetin and the yield of extract as investigation index,using multiple of adding liquid,soaking time,reflux time and extraction times as factors,water extraction technology of Chaihu anxin capsule was optimized by Box-Behnken response surface method based on single factor test. Validation test was conducted. RESULTS:The optimal extraction technology of Chaihu anxin capsules was adding 11 times of water,soaking for 10 h,extracting for 2 times,refluxing for 1.5 h each time. In validation test,the relative deviation of comprehensive scoring value to predicted value was 1.87% for 3 batches of samples(RSD<2%, n=3).CONCLUSIONS:The optimal extraction technology is simple,stable and suitable for further production of Chaihu anxin capsules.
引文
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[3]冯玉兰,柴薇薇,李健和,等.葛根素PEG-PE纳米胶束的制备及在急性心肌缺血模型小鼠体内的组织分布[J].中国药学杂志,2017,52(21):1918-1923.
[4]ZHANG X,XIONG J,LIU S,et al.Puerarin protects dopaminergic neurons in Parkinson’s disease models[J].Neuroscience,2014,280(7):88-98.
[5]WANG N,ZHANG YM,WU L,et al.Puerarin protected the brain from cerebral ischemia injury via astrocyte apoptosis inhibition[J].Neuropharmacology,2014.DOI:10.1016/j.neuropharm.2013.12.004.
[6]钱珺,谢凡,石燕红,等.HPLC法同时测定白芍配方颗粒中5种成分[J].中成药,2018,40(1):117-120.
[7]崔运浩,梁茂新,初杰.白芍潜在功能发掘与利用[J].中华中医药学刊,2016,34(5):1240-1243.
[8]刘冲,刘荫贞,乐智勇.桂枝饮片标准汤剂质量标准研究[J].中草药,2017,48(8):1577-1583.
[9]刘萍,张丽萍.桂枝化学成分及心血管药理作用研究[J].辽宁中医杂志,2012,39(10):1926-1927.
[10]皮胜玲,张凯强,胡玉珍,等.野生与栽培夏枯草5种有效成分的HPLC测定[J].中草药,2017,48(8):1666-1670.
[11]刘亚敏,栗俞程,李寒冰,等.夏枯草水提物抗抑郁作用研究[J].中药新药与临床药理,2017,28(4):1577-1583.
[12]单丽芳,杨红梅,曹蕾,等.多指标综合评分法优选参芪复方颗粒提取工艺及其热稳定性考察[J].中国实验方剂学杂志,2016,22(7):24-27.
[13]张志,李听弦,姚楠,等.多指标正交试验优化大黄的湿纸煨制工艺[J].中国药房,2018,29(7):964-967.
[14]范孟雪,秦昆明,丁斐,等.Box-Behnken响应面法结合多指标综合加权法优选升麻中酚酸类成分的提取工艺[J].中国药房,2016,27(13):1835-1838.
[15]陈恒文,田盼盼,何轩辉,等.多指标综合评分法优化稳心汤提取工艺[J].中国实验方剂学杂志,2018,24(20):25-31.
[16]王萍,王宇鹤,辛爱洁,等.Box-Behnken响应面法优化粉葛配方颗粒提取工艺[J].中成药,2017,39(6):1293-1296.