高寒草甸牧草内生解淀粉芽胞杆菌261MY6生防潜力评价
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摘要
本研究以番茄丁香假单胞叶斑病菌Pseudomonas syringa为指示菌,采用含菌平板抑菌圈测定法,从高寒草甸牧草内生细菌中筛选获取优良拮抗菌株261MY6,结合形态学、生理生化、16SrDNA及gyrB基因序列分析将其鉴定为解淀粉芽胞杆菌(Bacillus amyloliquefaciens)。通过平板对峙法、溶磷法、Salkowski法及盆栽试验等测定其生物功能及室内盆栽防效。结果表明,菌株261MY6对番茄丁香假单胞叶斑病菌的抑菌圈直径达1.20cm,盆栽防效达68.71%。具有溶解无机磷、固氮和分泌IAA的功能,对马铃薯褐腐病菌(Stysanus stemonitis)、马铃薯炭疽病菌(Colletotrichum coccodes)、马铃薯枯萎病菌(Fusarium avenaceum)、小麦根腐病菌(Bipolaris sorokiniana)、黄瓜枯萎病菌(F.oxysporum)、番茄灰霉病菌(Botrytis cinerea)、马铃薯褐腐病菌(Stysanus stemonitis)、孜然根腐病菌(F.Solani)和番瓜根腐病菌(Fusarium sp.)有良好抑制作用。该结果为解淀粉芽胞杆菌261MY6进一步开发利用提供了理论依据。
An antagonistic endophytic bacteria strain 261MY6,after assessing inhibition zones on nutrient media,was identified as having strain antagonistic ability against Pseudomonas Syirnge which causes bacteria spot on tomatoes.This strain was selected from endophytic bacteria strains that isolated from alpine meadow pasture.Based on morphological,physiological and biochemical analysis,plus16S rDNA and gyrB gene sequence analysis,it was identified as Bacillus amyloliquefaciens.The biological characteristics and functions were evaluated by means of plate sputum method,phosphate solubilizing method,Salkowski method and pot experiment.The strain 261MY6 produced a 1.20 cm diameter inhibition zone,and the potting control effect was 68.71%.It has the functions of dissolving nitrogen fixation,phosphorus solubilization,and IAA-producing ability.It also has a good inhibitory effect on the Stysanusstemonitis,Colletotrichum coccodes,Fusarium avenaceum,Bipolaris sorokiniana,F.oxysporum,Botrytis cinerea,Stysanus stemonitis,F.Solani,and Fusarium sp..These results suggest that further development and utilization of this endophytic bacteria strain 261MY6 should be undertaken.
An antagonistic endophytic bacteria strain 261MY6,after assessing inhibition zones on nutrient media,was identified as having strain antagonistic ability against Pseudomonas Syirnge which causes bacteria spot on tomatoes.This strain was selected from endophytic bacteria strains that isolated from alpine meadow pasture.Based on morphological,physiological and biochemical analysis,plus16S rDNA and gyrB gene sequence analysis,it was identified as Bacillus amyloliquefaciens.The biological characteristics and functions were evaluated by means of plate sputum method,phosphate solubilizing method,Salkowski method and pot experiment.The strain 261MY6 produced a 1.20 cm diameter inhibition zone,and the potting control effect was 68.71%.It has the functions of dissolving nitrogen fixation,phosphorus solubilization,and IAA-producing ability.It also has a good inhibitory effect on the Stysanusstemonitis,Colletotrichum coccodes,Fusarium avenaceum,Bipolaris sorokiniana,F.oxysporum,Botrytis cinerea,Stysanus stemonitis,F.Solani,and Fusarium sp..These results suggest that further development and utilization of this endophytic bacteria strain 261MY6 should be undertaken.
引文
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[17]冯中红,王玉琴,杨成德,等.番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价[J].草业学报,2015,24(08):166-173
[18]裴淑兰,刘东,刘慧芹,等.野生酸枣内生细菌筛选、鉴定及其抑菌活性测定[J].植物保护学报,2018,45(04):871-877
[19]乔俊卿,陈志谊,梁雪杰,等.枯草芽孢杆菌Bs916防治番茄青枯病[J].中国生物防治学报,2016,32(02):229-234
[20]谯天敏,张静,冉晓潇,等.解淀粉芽孢杆菌在山茶叶中的定殖及对山茶灰斑病的防效[J].西北农林科技大学学报(自然科学版),2015,43(10):77-84
[21]杨晓云,陈志谊,蒋盼盼,等.解淀粉芽孢杆菌B1619对番茄的促生作用[J].中国生物防治学报,2016,32(03):349-356
[2]Hardoim P R,van Overbeek L S,van Elsas J D.Properties of bacterial endophytes and their proposed role in plant growth[J].Trends in microbiology,2008,16(10):463-471
[3]Bulgarelli D,Schlaeppi K,Spaepen S,et al.Structure and functions of the bacterial microbiota of plants[J].Annual review of plant biology,2013(64):807-838
[4]Rashid S,Charles T C,Glick B R.Isolation and characterization of new plant growth-promoting bacterial endophytes[J].Applied Soil Ecology,2012,61(5):217-224
[5]江绪文,李贺勤,谭勇.藿香内生细菌HX-2的鉴定、耐性及对宿主植物的促生作用[J].草业学报,2018,27(01):161-168
[6]李春鸣,陈淑燕,徐长林,等.东祁连山高寒人工草地土壤种子库大小及物种构成[J].草地学报,2012,20(02):229-235
[7]魏立娟,王玉琴,杨成德,等.青海省海晏县线叶嵩草内生细菌的生物功能鉴定及测定[J].草地学报,2018,26(03):764-769
[8]崔月贞,万志文,冯疆蓉,等.东祁连山高寒草地优势牧草内生细菌产IAA能力的研究[J].草地学报,2016,24(03):618-623
[9]陈玉荣.农药污染现状与环境保护措施探究[J].环境与发展,2018,30(03):75-83
[10]张斯颖,骆焱平,胡坚,等.阴香内生菌YXG2-3的抑菌活性与生长条件优化[J].西北农林科技大学学报(自然科学版),2018,46(07):87-94
[11]郭海,杨成德,姚玉玲,等.高寒草地牧草内生细菌262AG6拮抗功能测定及鉴定[J].西北农业学报,2017,26(10):1529-1536
[12]赵健,兰成忠,陈庆河,等.抗番茄青枯病内生拮抗细菌的筛选及室内防效测定[J].武夷科学,2006(00):49-54
[13]孙崇思,陈晓敏,束长龙,等.对大丽轮枝菌具有拮抗作用的萎缩芽胞杆菌的分离和鉴定[J].植物保护,2014,40(01):30-37
[14]方中达.植病研究方法(第3版)[M].北京:中国农业出版社,1998:196-211
[15]东秀珠,蔡妙英,等.常见细菌鉴定手册[M].科学出版社,2001:370-398
[16]汪汉成,王茂胜,黄艳飞,等.烟草青枯病拮抗菌株X-60的分离鉴定及其表型组学分析[J].植物病理学报,2016,46(03):409-419
[17]冯中红,王玉琴,杨成德,等.番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价[J].草业学报,2015,24(08):166-173
[18]裴淑兰,刘东,刘慧芹,等.野生酸枣内生细菌筛选、鉴定及其抑菌活性测定[J].植物保护学报,2018,45(04):871-877
[19]乔俊卿,陈志谊,梁雪杰,等.枯草芽孢杆菌Bs916防治番茄青枯病[J].中国生物防治学报,2016,32(02):229-234
[20]谯天敏,张静,冉晓潇,等.解淀粉芽孢杆菌在山茶叶中的定殖及对山茶灰斑病的防效[J].西北农林科技大学学报(自然科学版),2015,43(10):77-84
[21]杨晓云,陈志谊,蒋盼盼,等.解淀粉芽孢杆菌B1619对番茄的促生作用[J].中国生物防治学报,2016,32(03):349-356