黄连和葛根联合抗糖尿病的药效学研究
|
摘要
目的:研究"B-P合剂"的降血糖活性(由黄连和葛根按1∶5配伍组成),为其临床应用提供实验依据。方法:以高脂高糖饲料饲养并结合链脲佐霉素(STZ)致高血糖SD大鼠为研究对象,对造模成功的大鼠分别灌胃给药高、中、低剂量的"B-P合剂",对照组和模型组给予等量的生理盐水,通过检测大鼠的体质量、胰岛素水平及胰岛素抵抗指数、血脂四项、空腹血糖和耐糖量、以及肝、肾功能指标检测和病理学检查,探究"B-P合剂"的降血糖活性。结果:"B-P合剂"可显著降低糖尿病模型大鼠的体质量、胰岛素抵抗指数、空腹血糖,回调血脂四项,改善大鼠的耐糖量,并且对肝、肾功能无明显影响。结论:"B-P合剂"具有较强的降血糖、降血脂活性,且安全无毒,值得大力开发应用为全新降糖药。
Objective:To study the hypoglycemic activity of B-P mixture(Coptidis Rhizoma and Pueraria lobata by 1∶5) to provide experimental basis for its clinical application. Methods: High-fat and high-sugar diets fed with streptozotocin( STZ)-induced hyperglycemia in Sprague-Dawley rats were used as study objects. High,medium,and lowdose BP mixtures were administered intragastrically to the successful rats. The control group and the model group were given the same amount of physiological saline. The body weight,insulin level,insulin resistance index,four lipids,fasting blood glucose and glucose tolerance,liver and kidney function tests,and pathological examination were measured to explore the hypoglycemic activity of BP mixture. Results: B-P mixture can significantly reduce the body weight,insulin resistance index,fasting blood glucose,and blood lipids of diabetic rats,improve the glucose tolerance of rats,and has no significant effect on liver and kidney function. Conclusion: B-P mixture has a strong hypoglycemic,hypolipidemic activity,safe and non-toxic. It is worth developing and applying as a new hypoglycemic agent.
Objective:To study the hypoglycemic activity of B-P mixture(Coptidis Rhizoma and Pueraria lobata by 1∶5) to provide experimental basis for its clinical application. Methods: High-fat and high-sugar diets fed with streptozotocin( STZ)-induced hyperglycemia in Sprague-Dawley rats were used as study objects. High,medium,and lowdose BP mixtures were administered intragastrically to the successful rats. The control group and the model group were given the same amount of physiological saline. The body weight,insulin level,insulin resistance index,four lipids,fasting blood glucose and glucose tolerance,liver and kidney function tests,and pathological examination were measured to explore the hypoglycemic activity of BP mixture. Results: B-P mixture can significantly reduce the body weight,insulin resistance index,fasting blood glucose,and blood lipids of diabetic rats,improve the glucose tolerance of rats,and has no significant effect on liver and kidney function. Conclusion: B-P mixture has a strong hypoglycemic,hypolipidemic activity,safe and non-toxic. It is worth developing and applying as a new hypoglycemic agent.
引文
[1] 时政,刘月.黄连素和二甲双胍二联用药治疗二型糖尿病的Meta分析[J].成都大学学报,2017,36(3):238-242.
[2] Wang LM,Gao P,Zhang M,et al. Prevalence and ethnic pattern of diabetes and prediabetes in China in 2013[J]. JAMA,2017,317(24):2515.
[3] 李骋,何金枝.黄连素调控胰岛素抵抗相关 2 型糖尿病的研究进展[J].中国中药杂志,2017,42(12):2254-2260.
[4] Tai N,Wong FS,Wen L. The role of gut microbiota in the de-velopment of type 1,type 2 diabetes mellitus and obesity[J].Rev Endocr Metab Disord,2015,16(1):55.
[5] International Diabetes Federation. IDF Diabetes Atlas 6th Edition [EB/OL]. [2016-01-01]. http://www.Idf.org/sites/default/files/EN-6E-Atlas-Full-0.pdf.
[6] Li M,Shu X,Xu H,et al. Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds[J]. J Transl Med,2016,14(1):237.
[7] Yang Y T,Liu S Y,He Y L,et al. Effect of Longzhang gargle on biofilm formation and acidogenicity of Streptococcus mutans in vitro[J]. Biomed Res Int,2016,20(16):582-585.
[8] 胡诚毅,莫志贤.黄连素的药理作用及机制研究进展[J]. 中国实验方剂学杂志,2017,23(20):213-219.
[9] Lan J. Meta-analysis of the effect and safety of berberine in the treatment of type 2 diabetes mellitus,hyperlipemia and hypertension[J].J Ethnopharmacol,2015,161:69-81.
[10] Li M,Shu X,Xu H,et al. Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds[J]. J Transl Med,2016,14(1):237.
[11] Sun H,Wang N,Cang Z,et al. Modulation of microbiota-gut-brain axis by berberine resulting in improved metabolic status in high-fat diet-fed rats[J]. Obes Facts,2016,9(6):365.
[12] 刘瑶,李伟.葛根素治疗糖尿病肾病的研究进展[J].中草药,2018,49(4):981-986.
[13] 朱水兰, 黎宇, 严奉东, 等. 油酸诱导胰岛素抵抗HepG2 细胞模型优化及小檗碱、黄芩苷、葛根素和甘草苷的体外降糖作用研究 [J]. 药物评价研究, 2017,40(10): 1402-1407.
[14] Sharen G, Bao L, Ma R, et al. Hypolipidemic effect of puerarin and underlying mechanism investigation [J]. Cell Biol, 2016, 4(6): 40-48.
[15] Han EB,Chang BY,Kim DS,et al.Melanogenesis inhibitory effect of aerial part of Pueraria thunbergiana in vitro and in vivo[J].Archives of Dermatological Research,2015,307(1):57-72.
[16] 潘欣萍,邢自力.茶多糖降糖活性中心 AN 蛋白组分的制备筛选及体内外降血糖实验研究[J]. 中国中药杂志,2018,43(4):736-742.
[17] 丘琴,陈明伟.匙羹藤果水提液降血糖作用及其作用机制[J].中国实验方剂学杂志,2017,23(14):158-163.
[18] HAHM SW, PARK J, SON YS. Opuntia humifusa stems lower bloodglucose and cholesterol levels in streptozotocin-induced diabetic rat[J].Nutrition Research, 2011, 31(6): 479-487.
[19] 卢福恭,王汉卿.消渴病历代学术考证[J]. 辽宁中医药大学学报,2018,20(5):20-22.
[20] 郭霭春,吴仕骥.黄帝内经素问校注[M].北京:人民卫生出版社,2013.
[21] 张韫,赵宗江.黄连素对 2 型糖尿病大鼠脂肪肝的治疗作用及机制研究[J]. 中日友好医院学报,2018,32(1):23-27.
[22] 胡娟,王旖.石斛三七降糖散对 KK- Ay 小鼠的降糖降脂作用研究[J]. 中药药理与临床, 2018,34(1):155-158.
[23] 翁思颖,柴可夫,周建扬.2型糖尿病合并非酒精性脂肪肝中医体质分布规律与基因多态性关系研究 [J]. 中华中医药杂志, 2017, 32(5):2216-2219.
[2] Wang LM,Gao P,Zhang M,et al. Prevalence and ethnic pattern of diabetes and prediabetes in China in 2013[J]. JAMA,2017,317(24):2515.
[3] 李骋,何金枝.黄连素调控胰岛素抵抗相关 2 型糖尿病的研究进展[J].中国中药杂志,2017,42(12):2254-2260.
[4] Tai N,Wong FS,Wen L. The role of gut microbiota in the de-velopment of type 1,type 2 diabetes mellitus and obesity[J].Rev Endocr Metab Disord,2015,16(1):55.
[5] International Diabetes Federation. IDF Diabetes Atlas 6th Edition [EB/OL]. [2016-01-01]. http://www.Idf.org/sites/default/files/EN-6E-Atlas-Full-0.pdf.
[6] Li M,Shu X,Xu H,et al. Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds[J]. J Transl Med,2016,14(1):237.
[7] Yang Y T,Liu S Y,He Y L,et al. Effect of Longzhang gargle on biofilm formation and acidogenicity of Streptococcus mutans in vitro[J]. Biomed Res Int,2016,20(16):582-585.
[8] 胡诚毅,莫志贤.黄连素的药理作用及机制研究进展[J]. 中国实验方剂学杂志,2017,23(20):213-219.
[9] Lan J. Meta-analysis of the effect and safety of berberine in the treatment of type 2 diabetes mellitus,hyperlipemia and hypertension[J].J Ethnopharmacol,2015,161:69-81.
[10] Li M,Shu X,Xu H,et al. Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds[J]. J Transl Med,2016,14(1):237.
[11] Sun H,Wang N,Cang Z,et al. Modulation of microbiota-gut-brain axis by berberine resulting in improved metabolic status in high-fat diet-fed rats[J]. Obes Facts,2016,9(6):365.
[12] 刘瑶,李伟.葛根素治疗糖尿病肾病的研究进展[J].中草药,2018,49(4):981-986.
[13] 朱水兰, 黎宇, 严奉东, 等. 油酸诱导胰岛素抵抗HepG2 细胞模型优化及小檗碱、黄芩苷、葛根素和甘草苷的体外降糖作用研究 [J]. 药物评价研究, 2017,40(10): 1402-1407.
[14] Sharen G, Bao L, Ma R, et al. Hypolipidemic effect of puerarin and underlying mechanism investigation [J]. Cell Biol, 2016, 4(6): 40-48.
[15] Han EB,Chang BY,Kim DS,et al.Melanogenesis inhibitory effect of aerial part of Pueraria thunbergiana in vitro and in vivo[J].Archives of Dermatological Research,2015,307(1):57-72.
[16] 潘欣萍,邢自力.茶多糖降糖活性中心 AN 蛋白组分的制备筛选及体内外降血糖实验研究[J]. 中国中药杂志,2018,43(4):736-742.
[17] 丘琴,陈明伟.匙羹藤果水提液降血糖作用及其作用机制[J].中国实验方剂学杂志,2017,23(14):158-163.
[18] HAHM SW, PARK J, SON YS. Opuntia humifusa stems lower bloodglucose and cholesterol levels in streptozotocin-induced diabetic rat[J].Nutrition Research, 2011, 31(6): 479-487.
[19] 卢福恭,王汉卿.消渴病历代学术考证[J]. 辽宁中医药大学学报,2018,20(5):20-22.
[20] 郭霭春,吴仕骥.黄帝内经素问校注[M].北京:人民卫生出版社,2013.
[21] 张韫,赵宗江.黄连素对 2 型糖尿病大鼠脂肪肝的治疗作用及机制研究[J]. 中日友好医院学报,2018,32(1):23-27.
[22] 胡娟,王旖.石斛三七降糖散对 KK- Ay 小鼠的降糖降脂作用研究[J]. 中药药理与临床, 2018,34(1):155-158.
[23] 翁思颖,柴可夫,周建扬.2型糖尿病合并非酒精性脂肪肝中医体质分布规律与基因多态性关系研究 [J]. 中华中医药杂志, 2017, 32(5):2216-2219.