不同类型油茶穗条提取液中生根抑制物的研究
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摘要
为给油茶无性繁殖提供理论依据,以油茶新品种‘秋霞’为试验材料,对不同月份(4月、6月)采集的不同类型穗条(8年生嫁接大树、3年生组培苗、3年生家系苗、3年生扦插苗)提取液的酸度与酚类物质的含量进行了检测与分析;并以白菜种子为试材,就不同类型穗条提取液对其发芽的生物效应进行了鉴定试验。测定结果表明:不同月份采集的不同类型穗条提取液的酸度和酚类物质含量均不同。鉴定试验结果表明:白菜种子在不同月份采集的不同类型穗条提取液原液中的发芽率不存在显著差异,但白菜种子在嫁接大树穗条提取液中的发芽率最低,而在家系苗穗条提取液中的发芽率最高。研究结果表明:不同类型穗条提取液中均存在生根抑制物质,且均存在酸类和酚类物质;嫁接大树穗条中生根抑制物质的含量较高,而家系苗穗条中生根抑制物质的含量较低。
In order to provide some references for vegetative propagation in Camellia oleifera, using new variety ‘qiuxia'as test materials, acidities and phenols contents in extracts of different types of scions(8-year-old grafted trees, 3-yearold tissue culture seedlings, 3-year-old family seedlings and 3-year-old cutting seedlings) were determined and analyzed,which were sampled at different periods(April and June). Biological effects of extracts of different types of scions on germination of Chinese cabbage seeds were identified. The results showed that acidities and phenols contents in different types of scions extracts at different periods were different, and germination rates of Chinese cabbage seeds in different types of scion extracts at different periods had no significant difference. Seed germination rate in scion extracts from grafted trees was the lowest, while that in scion extracts from family seedlings was the highest. There were rooting inhibitor in different types of scions extracts, and there were acids and phenols. Content of rooting inhibitor in scions from grafted trees was higher, and that in scions from family seedlings was lower.
In order to provide some references for vegetative propagation in Camellia oleifera, using new variety ‘qiuxia'as test materials, acidities and phenols contents in extracts of different types of scions(8-year-old grafted trees, 3-yearold tissue culture seedlings, 3-year-old family seedlings and 3-year-old cutting seedlings) were determined and analyzed,which were sampled at different periods(April and June). Biological effects of extracts of different types of scions on germination of Chinese cabbage seeds were identified. The results showed that acidities and phenols contents in different types of scions extracts at different periods were different, and germination rates of Chinese cabbage seeds in different types of scion extracts at different periods had no significant difference. Seed germination rate in scion extracts from grafted trees was the lowest, while that in scion extracts from family seedlings was the highest. There were rooting inhibitor in different types of scions extracts, and there were acids and phenols. Content of rooting inhibitor in scions from grafted trees was higher, and that in scions from family seedlings was lower.
引文
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[20]欧阳芳群,付国赞,王军辉,等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,51(3):155-162.
[21]赵敏,王炎,张伟.北五味种子内源抑制物质活性的消长规律[J].东北林业大学报,1999,27(3):44-47.
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[27]CLAIR J B.Juvenility and serial vegetative propagation of Norway spruce clones[J].Silvae Genetica,1985,34(1):42-48.
[2]温如斯,蔡娅,周招娣,等.4种砧木对普通油茶‘岑软3号’生长发育的影响[J].经济林研究,2018,36(4):73-79.
[3]王瑞,陈隆升,彭映赫,等.油茶新品种‘晚霞’嫁接亲和性研究[J].中南林业科技大学学报,2018,38(3):9-12,19.
[4]王湘南,王瑞,陈隆升,等.油茶新品种芽苗砧嫁接砧穗组合的亲和性分析[J].中南林业科技大学学报,2017,37(12):1-6.
[5]朱鹏,徐建民.邓恩桉组织材料生根抑制物研究[J].安徽农业科学,2007,35(32):10236-10238,10560.
[6]梁小春,项东云,姚瑞玲,等.邓恩桉插穗生根抑制物初步研究[J].广西林业科学,2010,39(1):14-16.
[7]黄卓烈,林韶湘,谭绍满.桉树体内的生根抑制物质研究综述[J].林业科学研究,1994,7(3):319-324.
[8]黄卓烈,林韶湘,谭绍满,等.尾叶桉等植物叶提取液对几种植物插条生根和种子萌发的影响[J].林业科学研究,1997,10(5):546-550.
[9]巫光宏,詹福建,黄卓烈,等.桉树LH21无性系生根抑制物初步研究[J].亚热带植物科学,2002,31(3):1-5.
[10]刘卫东,周莹,孙汉洲.桉树扦插生根过程中抑制物的研究[J].经济林研究,1998,16(4):16-19.
[11]詹福建,巫光宏,黄卓烈,等.桉树LH22、U6无性系茎提取液对绿豆插条发根和种子萌发的影响[J].植物研究,2003,23(2):172-177.
[12]程广有.紫杉插穗中生根抑制物的鉴定[J].北华大学学报(自然科学版),2000,1(2):163-166.
[13]季孔庶,王章荣,陈天华,等.马尾松插穗内源抑制物质的研究[J].林业科学,1997,33(2):142-151.
[14]季孔庶,王章荣,陈天华,等.马尾松种源与内源生根抑制物的相关性[J].南京林业大学学报(自然科学版),2002,26(2):24-28.
[15]于海莲,李凤兰,赵翠格,等.南方红豆杉种子发芽抑制物质的初步研究[J].北京林业大学学报,2009,31(5):78-83.
[16]徐程扬,张忠辉,李绍臣.核桃楸枝条、插穗中生根抑制物质的含量[J].吉林林学院学报,1998,14(4):212-215.
[17]魏丽秀,李培旺,张良波,等.穗条中的内含物对山苍子扦插繁殖的影响[J].经济林研究,2017,35(4):141-146.
[18]王芳,谢健,王泽,等.梭梭枝条生根抑制物研究[J].西北农业学报,2016,25(1):136-141.
[19]李永进,丁贵杰.不同家系马尾松插穗内源生根抑制物的分离、纯化及鉴定[J].浙江林学院学报,2010,27(4):507-512.
[20]欧阳芳群,付国赞,王军辉,等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化[J].林业科学,2015,51(3):155-162.
[21]赵敏,王炎,张伟.北五味种子内源抑制物质活性的消长规律[J].东北林业大学报,1999,27(3):44-47.
[22]赵敏.防风种子中内源抑制物质活性的研究[J].中草药,2004,35(4):441-444.
[23]黄卓烈,林韶湘,谭绍满,等.尾叶桉等植物茎提取液对绿豆等植物插条发根和种子萌发的影响[J].华南农业大学学报,1997,11(1):97-102.
[24]彭映赫,唐炜,袁毅,等.品种和穗条类型对油茶穗条中生根抑制物的影响[J].湖南林业科技,2017,44(6):70-74.
[25]王瑞,陈隆升,王湘南,等.4个油茶新品种抑制物质对扦插生根的影响[J].西南林业大学学报,2018,38(1):207-210.
[26]吴啸峰.红豆杉种子抑制物质的初步研究[J].植物生理学通讯,1985(4):23-26.
[27]CLAIR J B.Juvenility and serial vegetative propagation of Norway spruce clones[J].Silvae Genetica,1985,34(1):42-48.