三色堇种子活力测定方法研究
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摘要
研究三色堇种子活力适宜的测定方法,为选择高质量的三色堇种子提供技术支持。以‘P-1001’为材料,测定标准发芽试验、冷浸发芽试验及温室穴盘播种试验的种子萌发与种苗生长指标和不同浸泡时间的相对电导率,并研究各指标与温室穴盘播种试验的实际发芽指标之间的相关性。结果表明,冷浸发芽试验发芽率与温室穴盘播种发芽率以及种苗长满穴盘时计算的种子活力极显著相关;种苗长满穴盘时根长与标准发芽试验的苗长极显著相关;不同浸泡时间的三色堇种子的相对电导率可以用来预测温室穴盘播种的发芽率、种苗长满穴盘时计算的种子活力和根长、以及种子发芽率不变化时种子的活力和根长。建立了回归方程对温室发芽率、温室发芽的活力进行预测。
This research is to explore the method for testing the seed vigor of Viola tricolor L. so as to provide technical support for screening quality Viola tricolor L. seeds."P-1001 "was chosen as the experiment material. The standardgermination test(SGT),submerged cold test(SCT) and seed tray germination in greenhouse were conducted,seedling growth indexes recorded,the relative electrical conductivity(REC) examined,based upon which,correlation analysis was made,comparing indexes in SGT and SCT with the seed tray germination indexes. The results showed that the germination rate in SCT had a significant correlation to germination rate in greenhouse and to seed vigor evaluated when the seedlings were full of hole. The root length grown in greenhouse had a significant correlation with the seedling length with a maximum correlation level. The REC of seeds under different submerging time could predict the germination status in green house,including the vigor and the root length evaluated both at the times when the seedlings were full of hole with the germination rate reaching maximum level,and when the germination rate remained constant. Furthermore,regression equations were built to predict seed germination rate and vigor in green house.
This research is to explore the method for testing the seed vigor of Viola tricolor L. so as to provide technical support for screening quality Viola tricolor L. seeds."P-1001 "was chosen as the experiment material. The standardgermination test(SGT),submerged cold test(SCT) and seed tray germination in greenhouse were conducted,seedling growth indexes recorded,the relative electrical conductivity(REC) examined,based upon which,correlation analysis was made,comparing indexes in SGT and SCT with the seed tray germination indexes. The results showed that the germination rate in SCT had a significant correlation to germination rate in greenhouse and to seed vigor evaluated when the seedlings were full of hole. The root length grown in greenhouse had a significant correlation with the seedling length with a maximum correlation level. The REC of seeds under different submerging time could predict the germination status in green house,including the vigor and the root length evaluated both at the times when the seedlings were full of hole with the germination rate reaching maximum level,and when the germination rate remained constant. Furthermore,regression equations were built to predict seed germination rate and vigor in green house.
引文
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[12]于福来,刘凤波,王文全,等.甘草种苗质量分级标准研究[J].中国现代中药,2012(12):36-39.
[13]赵光武,王建华.甜玉米种子活力测定及其田间成苗能力的评估[J].植物生理学通讯,2005,41(4):444-448.
[14]叶凤林,李琳,杨丽明,等.应用机械视觉技术筛选射干种子精选指标的研究[J].中国农业大学学报,2016,21(8):119-124.
[15]叶凤林,李琳,杨丽明,等.基于机械视觉的黄芩种子精选技术研究[J].种子,2016,35(11):100-104.
[2]陈蕾太,孙爱清,杨敏,等.基于小麦种子发芽逆境抗逆指数的种子活力评价[J].应用生态学报,2016,27(9):2968-2974.
[3]杨冬风,尹淑欣,姜丽,等.玉米种子活力近红外光谱只能检测方法研究[J].核农学报,2013,27(7):957-961.
[4]唐强,宋自力,徐雯.枫杨种子活力测定方法研究[J].湖南林业科技,2011,38(2):14-16.
[5]邓飞,祁享年,赵光武.基于计算机图像处理技术评估杉木和马尾松种子活力[J].农业工程学报,2012,28(S2):274-279.
[6]边子星,颜彩燕,姚肖健,等.濒危华石斛种子活力测定方法研究[J].热带作物学报,2017,38(3):403-407.
[7]王开冻,魏跃.矮牵牛、三色堇种子发芽特性研究[J].种子,2007,26(2):89-91.
[8]王涛,张西西.低能氮离子注入三色堇、角堇种子的生物学效应[J].种子,2012,31(4):43-45.
[9]刘振威,贾文庆,孙丽,等. LED光源不同光源对三色堇种子萌发及幼苗生长的影响[J].贵州农业科学,2017,45(1):103-106.
[10]李瑞杰,陈垣,郭凤霞,等.素花党参种苗质量分级标准研究[J].中国中药杂志,2012,37(20):3041-3046.
[11]唐玲,张丽霞,王云强,等.金钗石斛种苗分级质量标准研究[J].中药材,2012,35(1):12-15.
[12]于福来,刘凤波,王文全,等.甘草种苗质量分级标准研究[J].中国现代中药,2012(12):36-39.
[13]赵光武,王建华.甜玉米种子活力测定及其田间成苗能力的评估[J].植物生理学通讯,2005,41(4):444-448.
[14]叶凤林,李琳,杨丽明,等.应用机械视觉技术筛选射干种子精选指标的研究[J].中国农业大学学报,2016,21(8):119-124.
[15]叶凤林,李琳,杨丽明,等.基于机械视觉的黄芩种子精选技术研究[J].种子,2016,35(11):100-104.