利用秋水仙碱诱导美洲黑杨2n花粉的初步研究
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摘要
为了获得美洲黑杨三倍体植株,本试验采用二因素随机区组试验设计,对利用秋水仙碱诱导美洲黑杨2n花粉技术进行研究,结果表明:(1)水培3 d、4 d,5 d时,利用0.3%、0.5%、0.8%的秋水仙碱对美洲黑杨雄花芽进行处理均能得到一定比例的2n花粉;(2)在水培3 d时,利用0.5%的秋水仙碱处理美洲黑杨雄花芽,得到的2n花粉最多,为23.4%。
In order to get the triploids of P. deltoid,the technique was researched to induce 2 n pollen of P. deltoid by colchicines by two-factor randomized block design. The results indicated that( 1) a certain proportion of 2 n pollen grains could be gotten from the male buds induced by cochicine solution with the concentration of 0. 3%,0. 5% and 0. 8%,under the condition of water culture for 3,4 and 5 days.(2)the proportion of 2 n pollen grains could be the highest when the male buds were treated by the cochicine solution of 0. 5% under the condition of water culture for 3 days,the percentage of A4( d ≧ 50) could be up to 1. 4%,the percentage of 2 n pollen( d ≧ 37. 5) could be up to 23. 4%.
In order to get the triploids of P. deltoid,the technique was researched to induce 2 n pollen of P. deltoid by colchicines by two-factor randomized block design. The results indicated that( 1) a certain proportion of 2 n pollen grains could be gotten from the male buds induced by cochicine solution with the concentration of 0. 3%,0. 5% and 0. 8%,under the condition of water culture for 3,4 and 5 days.(2)the proportion of 2 n pollen grains could be the highest when the male buds were treated by the cochicine solution of 0. 5% under the condition of water culture for 3 days,the percentage of A4( d ≧ 50) could be up to 1. 4%,the percentage of 2 n pollen( d ≧ 37. 5) could be up to 23. 4%.
引文
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[2]LINNAEUS C.Populus.Linn.Sp.Pl.[M].London:Bernard Quaritch Ltd,1753:1034~1035.
[3]李云.杨树三倍体选育研究进展[J].植物学通报,2001,18(4):451~458.
[4]Johnsson H,Eklundh C.Colchicine treatment as a method in breeding hardwood species.Svensk Papp Tidn,1940,43:373~377.
[5]张志毅,李凤兰.白杨染色体加倍技术研究及三倍体育种(Ⅰ)花粉染色体加倍技术.北京林业大学学报,1992,14(增刊3):52~58.
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[11]李开隆,肖静,刘桂丰,等.秋水仙素处理诱导大青杨2n花粉方法的优化[J].核农学报,2006,20(4):282~286.
[12]王君,康向阳,李代丽,等.授粉后施加秋水仙碱处理诱导青杨派异源三倍体[J].北京林业大学学报,2007,29(5):18~21.
[13]祁传磊.大青杨多倍体的诱导.硕士学位论文.东北林业大学.pp1-9.
[14]石乐,张平冬,毛彦科,等.高温处理诱导染色体加倍获得白杨杂种多倍体[J].核农学报,2012,26(8):1118~1123.
[15]李静,李春明,卓泳杉,等.秋水仙碱诱导山杨异源多倍体[J].安徽农业科学,2014,42(4):1098~1100.
[16]朱之悌,康向阳,等.毛白杨天然三倍体选种研究.林业科学,1998,34(4):22~31.
[17]朱之悌,林惠斌,等.毛白杨异源三倍体B301等无性系选育的研究.林业科学,1995,31(6):499~505.
[18]Ehrendorfer F.Polyploidy and distribution[J].Basic Life Sci,1979,13:45~60.
[19]Ramsey J,Schemske D W.Neopolyploidy in flowering plants[J].Ann Rev Ecol Syst,2002,33:589~639.
[20]Johnsson H.Ten years'aspen breeding by the Swedish Forest Tree Breeding Association.Medd Foren Vaxtforadl Skogstrad,No.46,pp.9.Repr Svensk Papp Tidn 1947,50:17~20.
[21]Johnsson H.Two newspaper cuttings on the giant aspen.Skogen,1948,35(9):107.
[22]Johnsson H.Development of triploid and diploid populous tremula during the juvenile period[J].Z Forstgenet,1953,2(4):73~77.
[2]LINNAEUS C.Populus.Linn.Sp.Pl.[M].London:Bernard Quaritch Ltd,1753:1034~1035.
[3]李云.杨树三倍体选育研究进展[J].植物学通报,2001,18(4):451~458.
[4]Johnsson H,Eklundh C.Colchicine treatment as a method in breeding hardwood species.Svensk Papp Tidn,1940,43:373~377.
[5]张志毅,李凤兰.白杨染色体加倍技术研究及三倍体育种(Ⅰ)花粉染色体加倍技术.北京林业大学学报,1992,14(增刊3):52~58.
[6]康向阳,朱之悌.白杨2n花粉生命力测定方法及萌发特征的研究[J].云南植物研究,1997,19(4):402~406.
[7]康向阳,朱之悌,林惠斌.杨树花粉染色体加倍有效处理时期的研究[J].林业科学,1999,39(4):21~24.
[8]康向阳,朱之悌,张志毅.银腺杨与毛新杨正反交三倍体选育[J].北京林业大学学报,2000,22(6):8~11.
[9]康向阳,朱之悌,林惠斌.白杨不同倍性花粉的辐射敏感性及其应用[J].遗传学报,2000,27(1):78~82.
[10]李云,朱之悌,田砚亭,等.秋水仙碱处理白杨雌花芽培育三倍体植株的研究[J].林业科学,2001,37(5):68~74.
[11]李开隆,肖静,刘桂丰,等.秋水仙素处理诱导大青杨2n花粉方法的优化[J].核农学报,2006,20(4):282~286.
[12]王君,康向阳,李代丽,等.授粉后施加秋水仙碱处理诱导青杨派异源三倍体[J].北京林业大学学报,2007,29(5):18~21.
[13]祁传磊.大青杨多倍体的诱导.硕士学位论文.东北林业大学.pp1-9.
[14]石乐,张平冬,毛彦科,等.高温处理诱导染色体加倍获得白杨杂种多倍体[J].核农学报,2012,26(8):1118~1123.
[15]李静,李春明,卓泳杉,等.秋水仙碱诱导山杨异源多倍体[J].安徽农业科学,2014,42(4):1098~1100.
[16]朱之悌,康向阳,等.毛白杨天然三倍体选种研究.林业科学,1998,34(4):22~31.
[17]朱之悌,林惠斌,等.毛白杨异源三倍体B301等无性系选育的研究.林业科学,1995,31(6):499~505.
[18]Ehrendorfer F.Polyploidy and distribution[J].Basic Life Sci,1979,13:45~60.
[19]Ramsey J,Schemske D W.Neopolyploidy in flowering plants[J].Ann Rev Ecol Syst,2002,33:589~639.
[20]Johnsson H.Ten years'aspen breeding by the Swedish Forest Tree Breeding Association.Medd Foren Vaxtforadl Skogstrad,No.46,pp.9.Repr Svensk Papp Tidn 1947,50:17~20.
[21]Johnsson H.Two newspaper cuttings on the giant aspen.Skogen,1948,35(9):107.
[22]Johnsson H.Development of triploid and diploid populous tremula during the juvenile period[J].Z Forstgenet,1953,2(4):73~77.