糖尿病视网膜病变患者ApoE等位基因多态性的研究
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摘要
目的探讨糖尿病视网膜病变患者及糖尿病未伴发视网膜病变患者ApoE等位基因多态性及其之间的关系。方法糖尿病患者根据是否伴发眼底病变分为两组:糖尿病视网膜病变组(DR组)和糖尿病未伴发视网膜病变组(NDR组)。应用PCR-荧光探针法分别检测两组患者的ApoE基因型并对结果进行统计学分析。结果 ApoE基因型在DR组和NDR组间差异无统计学意义(P>0.05),DR组ApoE等位基因频率ε2明显高于NDR组(P<0.05)。结论 ApoE基因型与糖尿病患者是否并发视网膜病变无关,ApoE等位基因ε2可能是糖尿病伴发视网膜病变的危险因素。
Objective To investigate the polymorphism of ApoE allele in the patients with and without diabetic retinopathy and its relationship.Methods The diabetic patients were divided into two groups according to whether complicating fundus lesions:diabetic retinopathy group(DR group)and DN group(NDR group).The ApoE genotypes in the two groups were detected by PCR-fluorescence probe and the results were statistically analyzed.Results There was no statistically significant difference in ApoE genotype between the DR group and NDR group(P>0.05),the ApoE ε2 allele frequency in the DR group was significantly higher than that in the NDR group(P<0.05).Conclusion ApoE genotype has no relation with whether complicating diabetic retinopathy,and ApoE ε2 allele may be a risk factor of diabetes complicating retinopathy.
Objective To investigate the polymorphism of ApoE allele in the patients with and without diabetic retinopathy and its relationship.Methods The diabetic patients were divided into two groups according to whether complicating fundus lesions:diabetic retinopathy group(DR group)and DN group(NDR group).The ApoE genotypes in the two groups were detected by PCR-fluorescence probe and the results were statistically analyzed.Results There was no statistically significant difference in ApoE genotype between the DR group and NDR group(P>0.05),the ApoE ε2 allele frequency in the DR group was significantly higher than that in the NDR group(P<0.05).Conclusion ApoE genotype has no relation with whether complicating diabetic retinopathy,and ApoE ε2 allele may be a risk factor of diabetes complicating retinopathy.
引文
[1]张凤俊,易敬林,李晶明,等.糖尿病视网膜病变发病机制研究进展[J].眼科新进展,2016,36(6):584-587.
[2]TAI L M,THOMAS R,MAROTTOLI F M,et al.The role of ApoE in cerebrovascular dysfunction[J].Acta Neuropathologica,2016,131(5):709-723.
[3]JIANG Y W,MA L,HAN C W,et al.Effects of apolipoprotein E isoforms in diabetic nephropathy of Chinese type 2diabetic patients[J].J Diabetes Res,2017(3):1-6.
[4]王艳梅,王思轩,武曦蔼,等.2型糖尿病周围神经病变与载脂蛋白E基因多态性的相关性研究[J].中国糖尿病杂志,2018,26(1):14-17.
[5]MOOYAART A L,VALK E,VAN ES L A,et al.Genetic associations in diabetic nephropathy:a meta-analysis[J].Diabetologia,2011,54(3):544-553.
[6]中华医学会糖尿病学会.中国2型糖尿病防治指南:2010年版[J].中国糖尿病杂志,2011,19(6):1227-1245.
[7]CUDABACK E,LI X W,MONTINE K S,et al.Apolipoprotein E isoform-dependent microglia migration[J].FASEB J,2011,25(6):2082-2091.
[8]姜永玮,马亮,刘倩,等.ApoE基因多态性与糖尿病肾病的相关性研究进展[J].中华检验医学杂志,2016,39(12):974-977.
[9]KHAN T A,SHAH T,PRIETO D,et al.Apolipoprotein Egenotype,cardiovascular biomarkers and risk of stroke:systematic review and meta-analysis of 14,015stroke cases and pooled analysis of primary biomarker data from up to 60 883individuals[J].Int J Epidemiol,2013,42(2):475-492.
[10]GU L,SU L,CHEN Q,et al.Association between the apolipoprotein E gene polymorphism and ischemic stroke in Chinese populations:new data and meta-analysis[J].Exp Ther Med,2013,5(3):853-859.
[11]吕小林,万腊根,刘淑媛.冠心病患者ApoE基因多态性及其脂质谱相关性研究[J].南昌大学学报(医学版),2017,57(5):20-22.
[12]HUANG Y D.Abeta-independent roles of apolipoprotein E4in the pathogenesis of Alzheimer′s disease[J].Trends Mol Med,2010,16(6):287-294.
[13]TAI L M,GHURA S,KOSTER K P,et al.APOE-modulated Aβ-induced neuroinflammation in Alzheimer′s disease:current landscape,novel data,and future perspective[J].J Neurochem,2015,133(4):465-488.
[14]YANG S H,DOU K F,SONG W J.Prevalence of diabetes among men and women in China[J].N Engl J Med,2010,362(25):2425-2426.
[15]金佩瑶,彭金娟,邹海东,等.上海市新泾社区2型糖尿病居民5年随访的前瞻性调查研究1.糖尿病视网膜病变和糖尿病黄斑水肿的发病率及危险因素[J].中华实验眼科杂志,2016,34(4):363-367.
[16]WANG F H,LIANG Y B,ZHANG F,et al.Prevalence of diabetic retinopathy in rural China:the Handan Eye Study[J].Ophthalmology,2009,116(3):461-467.
[2]TAI L M,THOMAS R,MAROTTOLI F M,et al.The role of ApoE in cerebrovascular dysfunction[J].Acta Neuropathologica,2016,131(5):709-723.
[3]JIANG Y W,MA L,HAN C W,et al.Effects of apolipoprotein E isoforms in diabetic nephropathy of Chinese type 2diabetic patients[J].J Diabetes Res,2017(3):1-6.
[4]王艳梅,王思轩,武曦蔼,等.2型糖尿病周围神经病变与载脂蛋白E基因多态性的相关性研究[J].中国糖尿病杂志,2018,26(1):14-17.
[5]MOOYAART A L,VALK E,VAN ES L A,et al.Genetic associations in diabetic nephropathy:a meta-analysis[J].Diabetologia,2011,54(3):544-553.
[6]中华医学会糖尿病学会.中国2型糖尿病防治指南:2010年版[J].中国糖尿病杂志,2011,19(6):1227-1245.
[7]CUDABACK E,LI X W,MONTINE K S,et al.Apolipoprotein E isoform-dependent microglia migration[J].FASEB J,2011,25(6):2082-2091.
[8]姜永玮,马亮,刘倩,等.ApoE基因多态性与糖尿病肾病的相关性研究进展[J].中华检验医学杂志,2016,39(12):974-977.
[9]KHAN T A,SHAH T,PRIETO D,et al.Apolipoprotein Egenotype,cardiovascular biomarkers and risk of stroke:systematic review and meta-analysis of 14,015stroke cases and pooled analysis of primary biomarker data from up to 60 883individuals[J].Int J Epidemiol,2013,42(2):475-492.
[10]GU L,SU L,CHEN Q,et al.Association between the apolipoprotein E gene polymorphism and ischemic stroke in Chinese populations:new data and meta-analysis[J].Exp Ther Med,2013,5(3):853-859.
[11]吕小林,万腊根,刘淑媛.冠心病患者ApoE基因多态性及其脂质谱相关性研究[J].南昌大学学报(医学版),2017,57(5):20-22.
[12]HUANG Y D.Abeta-independent roles of apolipoprotein E4in the pathogenesis of Alzheimer′s disease[J].Trends Mol Med,2010,16(6):287-294.
[13]TAI L M,GHURA S,KOSTER K P,et al.APOE-modulated Aβ-induced neuroinflammation in Alzheimer′s disease:current landscape,novel data,and future perspective[J].J Neurochem,2015,133(4):465-488.
[14]YANG S H,DOU K F,SONG W J.Prevalence of diabetes among men and women in China[J].N Engl J Med,2010,362(25):2425-2426.
[15]金佩瑶,彭金娟,邹海东,等.上海市新泾社区2型糖尿病居民5年随访的前瞻性调查研究1.糖尿病视网膜病变和糖尿病黄斑水肿的发病率及危险因素[J].中华实验眼科杂志,2016,34(4):363-367.
[16]WANG F H,LIANG Y B,ZHANG F,et al.Prevalence of diabetic retinopathy in rural China:the Handan Eye Study[J].Ophthalmology,2009,116(3):461-467.