刮痧对健康个体皮肤及系统免疫功能的调节研究
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摘要
目的从免疫学的角度研究和探讨刮痧治疗作用的机理。方法在Balb/c小鼠背部皮肤进行刮痧处理后,使用组织染色法观察皮肤组织形态的变化,用流式细胞术和ELISA分别测定局部免疫相关细胞的比例和局部及全身重要细胞因子的含量变化,并通过测定疫苗接种后的抗体滴度,来评估刮痧处理后机体的免疫响应能力。结果刮痧后的皮肤组织毛细血管扩张,红细胞外渗,局部微循环旺盛,免疫活性细胞比例增加,促炎性细胞因子上调,调节性细胞因子下降,表明刮痧能引起局部和全身性反应;并且刮痧处理能诱导机体产生约3倍高的抗原特异性抗体滴度,导致免疫应答倾向于Th1型。结论刮痧可以增强皮肤的微循环,加强其免疫响应的能力,增强和调节先天性免疫及适应性免疫功能,是其产生治疗效果的部分原因。
OBJECTIVE In this study,we investigate Gua Sha's therapeutic mechanism from immunological approaches.METHODS Gua Sha was performed on the back of Balb/c mice and the effects on the morphology of the skin tissue were observed using histological staining.Flow cytometry and ELISA were employed to determine the proportions of local immune active cells and local and systemic cytokine levels,respectively.Antigen-specific subtype antibody titers against intradermal and intranasal vaccines were utilized to assess Gua Sha treatment on the immunological functions of the skin locally and systematically.RESULTS Blood vessel expansion,erythrocyte extravasation and increased ratios of immune active cells were observed in the skin tissue following treatment,indicating an enhanced microcirculation.Pro-inflammatory cytokines were found up-regulated,and immunosuppressive cytokines downregulated,in both treated and untreated skin areas.These showed that Gua Sha can cause local and systemic effects.Intradermal delivery of a model antigen following Gua Sha treatment induced about threefold higher IgG titers with a more Th1-biased antibody subtype profile.CONCLUSION Gua Sha treatment can boost skin microcirculation,enhance and regulate the innate and adaptive immune functions of the skin,which contributes partially to its therapeutic effects.
OBJECTIVE In this study,we investigate Gua Sha's therapeutic mechanism from immunological approaches.METHODS Gua Sha was performed on the back of Balb/c mice and the effects on the morphology of the skin tissue were observed using histological staining.Flow cytometry and ELISA were employed to determine the proportions of local immune active cells and local and systemic cytokine levels,respectively.Antigen-specific subtype antibody titers against intradermal and intranasal vaccines were utilized to assess Gua Sha treatment on the immunological functions of the skin locally and systematically.RESULTS Blood vessel expansion,erythrocyte extravasation and increased ratios of immune active cells were observed in the skin tissue following treatment,indicating an enhanced microcirculation.Pro-inflammatory cytokines were found up-regulated,and immunosuppressive cytokines downregulated,in both treated and untreated skin areas.These showed that Gua Sha can cause local and systemic effects.Intradermal delivery of a model antigen following Gua Sha treatment induced about threefold higher IgG titers with a more Th1-biased antibody subtype profile.CONCLUSION Gua Sha treatment can boost skin microcirculation,enhance and regulate the innate and adaptive immune functions of the skin,which contributes partially to its therapeutic effects.
引文
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[17]杨进岗,袁德培.土家以酒为介质刮痧疗法对“感冒”模型大鼠IL-2及TNF-α含量的影响[J].中国民族民间医药,2015,24(2):2-3.
[2]贾曼,丰芬.刮痧疗法及其应用[J].河南中医,2011,31(12):1368-1370.
[3]王钰婷.刮痧配合外敷蜂胶治疗黄褐斑的临床研究[D].广州:中医药大学,2013.
[4]KWONG KK,KLOETZER L,WONG KK,et al.Bioluminescence imaging of heme oxygenase-1upregulation in the Gua Sha procedure[J].J Vis Exp,2009(30):e1385-e1385.
[5]LAUCHE R,WUBBELING K,LUDTKE R,et al.Randomized controlled pilot study:pain intensity and pressure pain thresholds in patients with neck and low back pain before and after traditional East Asian"gua sha"therapy[J].Am J Chin Med,2012,40(5):905-917.
[6]NIELSEN A.Gua Sha research and the language of integrative medicine[J].J Bodywork Movement Ther,2009,13(1):63-72.
[7]曾上劼.经络刮痧前后白细胞变化分析[J].黑龙江中医药,2003(1):41-42.
[8]DING Z,VERBAAN FJ,BIVAS-BENITA M,et al.Microneedle arrays for the transcutaneous immunization of diphtheria and influenza in BALB/c mice[J].J Control Releas,2009,136(1):71-78.
[9]NIELSEN A,KNOBLAUCH NT,DOBOS GJ,et al.The effect of Gua Sha treatment on the microcirculation of surface tissue:apilot study in healthy subjects[J].J Sci Heal,2007,3(5):456-466.
[10]WANG Y,OLIVER G.Current views on the function of the lymphatic vasculature in health and disease[J].Genes Develop,2010,24(19):2115-2126.
[11]MCFADYEN JD,KAPLAN ZS.Platelets are not just for clots[J].Transfusion Med Rev,2015,29(2):110-119.
[12]AFONSO LC,SCHARTON TM,VIEIRA LQ,et al.The adjuvant effect of interleukin-12in a vaccine against Leishmania major[J].Science,1994,263(5144):235-237.
[13]SINGH V,JAIN S,GOWTHAMAN U,et al.Co-administration of IL-1+IL-6+TNF-αwith Mycobacterium tuberculosis infected macrophages vaccine induces better protective T cell memory than BCG[J].PLoS ONE,2011,6(1):438-438.
[14]TOVEY MG,LALLEMAND C.Adjuvant Activity of Cytokines[M].Clifton:Humana Press,2010:287-309.
[15]EVANS SS,REPASKY EA,FISHER DT,et al.Fever and the thermal regulation of immunity:the immune system feels the heat[J].Nat Rev Immunol,2015,15(6):335-349.
[16]苏荣华,刘颖,王光义,等.通阳刮痧疗法对佐剂性关节炎大鼠TNF-α及T淋巴细胞亚群的影响[J].贵阳医学院学报,2015,40(9):919-921.
[17]杨进岗,袁德培.土家以酒为介质刮痧疗法对“感冒”模型大鼠IL-2及TNF-α含量的影响[J].中国民族民间医药,2015,24(2):2-3.