Effects of Di-n-butyl Phthalate and Diethyl Phthalate on Acetylcholinesterase Activity and Neuro-related Gene Expression in Embryonic Zebrafish
- 作者：Hai XuXiaoling ShaoZhen ZhangYanmin ZouXiangyang WuLiuqing Yang
- 会议时间：2013-12-01
- 关键词：Di-n-butyl phthalate ; Diethyl phthalate ; Developmental neurotoxicity ; Nervous system ; Zebrafish
- 作者单位：Hai Xu,Xiaoling Shao,Zhen Zhang,Yanmin Zou,Xiangyang Wu(School of the Environment,Jiangsu University,Xuefu Rd.301,Zhenjiang 212013,Jiangsu,China)Liuqing Yang(School of Chemistry and Chemical Engineering,Jiangsu University,Xuefu Rd.301,Zhenjiang 212013,Jiangsu,China)
- 母体文献：2013年水资源生态保护与水污染控制研讨会论文集
- 会议名称：2013年水资源生态保护与水污染控制研讨会
- 会议地点：哈尔滨
- 主办单位：中国环境科学学会
- 语种：chi
摘要
In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture.Four-hour post-fertilization (hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture (DBP-DEP) until 96 hpf.The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed.The results showed that transcripts of growth associated protein 43 (gap43), embryonic lethal abnormal vision-like 3 (elavl3), glial fibrillary acidic protein (gfap), myelin basic protein (mbp), al-tubulin and neurogeninl (ngnl) were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure.In addition, acetylcholinesterase (AChE) activity was significantly inhibited in the embryos.These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.