Exploring valid internal-control genes in Porphyra yezoensis (Bangiaceae) during stress response conditions

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• 作者：Wenlei Wang (1)
  Xiaojie Wu (1)
  Chao Wang (2)
  Zhaojun Jia (1)
  Linwen He (2)
  Yifan Wei (2)
  Jianfeng Niu (2) (3)
  Guangce Wang (1) (2)
  
• 关键词：constitutive expression gene ; housekeeping gene ; Porphyra yezoensis Ueda ; real ; time quantitative PCR ; stress responding
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：32
• 期：4
• 页码：783-791
• 全文大小：586 KB
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• 作者单位：Wenlei Wang (1)
  Xiaojie Wu (1)
  Chao Wang (2)
  Zhaojun Jia (1)
  Linwen He (2)
  Yifan Wei (2)
  Jianfeng Niu (2) (3)
  Guangce Wang (1) (2)
  
  1. College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China
  2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
  3. Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong, 226006, China
  
• ISSN：1993-5005

文摘

To screen the stable expression genes related to the stress (strong light, dehydration and temperature shock) we applied Absolute real-time PCR technology to determine the transcription numbers of the selected test genes in P orphyra yezoensis, which has been regarded as a potential model species responding the stress conditions in the intertidal. Absolute real-time PCR technology was applied to determine the transcription numbers of the selected test genes in P orphyra yezoensis, which has been regarded as a potential model species in stress responding. According to the results of photosynthesis parameters, we observed that Y(II) and F v/F m were significantly affected when stress was imposed on the thalli of P orphyra yezoensis, but underwent almost completely recovered under normal conditions, which were collected for the following experiments. Then three samples, which were treated with different grade stresses combined with salinity, irradiation and temperature, were collected. The transcription numbers of seven constitutive expression genes in above samples were determined after RNA extraction and cDNA synthesis. Finally, a general insight into the selection of internal control genes during stress response was obtained. We found that there were no obvious effects in terms of salinity stress (at salinity 90) on transcription of most genes used in the study. The 18S ribosomal RNA gene had the highest expression level, varying remarkably among different tested groups. RPS8 expression showed a high irregular variance between samples. GAPDH presented comparatively stable expression and could thus be selected as the internal control. EF-1α showed stable expression during the series of multiple-stress tests. Our research provided available references for the selection of internal control genes for transcripts determination of P. yezoensis.