Soluble Epoxide Hydrolase Inhibitor Suppresses the Expression of Triggering Receptor Expressed on Myeloid Cells-1 by Inhibiting NF-kB Activation in Murine Macrophage
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- 作者：Liang Dong ; Yong Zhou ; Zhao-Qiong Zhu ; Tian Liu ; Jia-Xi Duan ; Jun Zhang…
- 关键词：KEY WORDStriggering receptor expressed on myeloid cells ; 1 ; epoxyeicosatrienoic acids ; soluble epoxide hydrolase inhibitor ; macrophage ; inflammation
- 刊名：Inflammation
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：40
- 期：1
- 页码：13-20
- 全文大小：
- 刊物类别：Medicine
- 刊物主题：Rheumatology; Internal Medicine; Pharmacology/Toxicology; Pathology;
- 出版者：Springer US
- ISSN：1573-2576
- 卷排序：40
文摘
Triggering receptors expressed on myeloid cell-1 (TREM-1) is a superimmunoglobulin receptor expressed on myeloid cells. TREM-1 amplifies the inflammatory response. Epoxyeicosatrienoic acids (EETs), the metabolites of arachidonic acid derived from the cytochrome P450 enzyme, have anti-inflammatory properties. However, the effects of EETs on TREM-1 expression under inflammatory stimulation remain unclear. Therefore, inhibition of soluble epoxide hydrolase (sEH) with a highly selective inhibitor [1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea, TPPU] was used to stabilize EETs. LPS was intratracheally injected into mice to induce pulmonary inflammation, after TPPU treatment for 3 h. Histological examination showed TPPU treatment-alleviated LPS-induced pulmonary inflammation. TPPU decreased TREM-1 expression, but not DAP12 or MyD88 expression. Murine peritoneal macrophages were challenged with LPS in vitro. We found that TPPU reduced LPS-induced TREM-1 expression in a dose-dependent manner, but not DAP12 or MyD88 expression. TPPU also decreased downstream signal from TREM-1, reducing pro-inflammatory cytokine TNF-α and IL-1β mRNA expression. Furthermore, TPPU treatment inhibited IkB degradation in vivo and in vitro. Our results indicate that the inhibition of sEH suppresses LPS-induced TREM-1 expression and inflammation via inhibiting NF-kB activation in murine macrophage.