Protective effect of carvacrol from Thymus quinquecostatus Celak against tert-butyl hydroperoxide-induced oxidative damage in Chang cells

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• 作者：Yon-Suk Kim (1)
  Jin-Woo Hwang (1)
  Si-Heung Sung (4)
  Sun-Joo Park (2)
  Yong-Tae Kim (3)
  Eun-Kyung Kim (4)
  Sang-Ho Moon (4)
  Byong-Tae Jeon (4)
  Pyo-Jam Park (1) (4)
  
  1. Department of Biotechnology ; Konkuk University ; Chungju ; Chungbuk ; 380-701 ; Korea
  4. Nokyong Research Center ; Konkuk University ; Chungju ; Chungbuk ; 380-701 ; Korea
  2. Department of Chemistry ; Pukyoung National University ; Busan ; 608-737 ; Korea
  3. Department of Food Science and Biotechnology ; Kunsan National University ; Gunsan ; Jeonbuk ; 573-701 ; Korea
  
• 关键词：carvacrol ; antioxidant activity ; tert ; butyl hydroperoxide ; Chang cell ; reactive oxygen species
• 刊名：Food Science and Biotechnology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：24
• 期：2
• 页码：735-741
• 全文大小：394 KB
• 参考文献：1. Loguercio, C, Federico, A (2003) Oxidative stress in viral and alcoholic hepatitis. Free Radical Bio. Med. 34: pp. 1-10 CrossRef
  2. Arteel, GE, Kadiiska, MB, Rusyn, I, Bradford, BU, Mason, RP, Raleigh, JA, Thurman, RG (1999) Oxidative stress occurs in perfused rat liver at low oxygen tension by mechanisms involving peroxynitrite. Mol. Pharmacol. 55: pp. 708-715
  3. Hoek, JB, Cahill, A, Pastorino, JG (2002) Alcohol and mitochondria: A dysfunctional relationship. Gastroenterology 122: pp. 2049-2063 CrossRef
  4. Kevin, LG, Camara, AK, Riess, ML, Novalija, E, Stowe, DF (2003) Ischemic preconditioning alters real-time measure of O2 radicals in intact hearts with ischemia and reperfusion. Am. J. Physiol.-Heart C. 284: pp. H566-H574 CrossRef
  5. Kakkar, P, Singh, BK (2007) Mitochondria: A hub of redox activities and cellular distress control. Mol. Cell. Biochem. 305: pp. 235-253 CrossRef
  6. Kim, JA, Kang, YS, Kim, YO, Lee, SH, Lee, YS (1998) Role of Ca2+ influx in the tert-butyl hydroperoxide-induced apoptosis of HepG2 human hepatoblastoma cells. Exp. Mol. Med. 30: pp. 137-144 CrossRef
  7. Dumont, P, Burton, M, Chen, QM, Gonos, ES, Frippiat, C, Mazarati, JB, Eliaers, F, Remacle, J, Toussaint, O (2000) Induction of replicative senescence biomarkers by sublethal oxidative stresses in normal human fibroblast. Free Radical Bio. Med. 28: pp. 361-373 CrossRef
  8. Wang, CJ, Wang, JM, Lin, WL, Chu, CY, Chou, FP, Tseng, TH (2000) Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem. Toxicol. 38: pp. 411-416 CrossRef
  9. Lin, WL, Wang, CJ, Tsai, YY, Liu, CL, Hwang, JM, Tseng, TH (2000) Inhibitory effect of esculetin on oxidative damage induced by t-butyl hydroperoxide in rat liver. Arch. Toxicol. 74: pp. 467-472 CrossRef
  10. Minotti, G, Borrello, S, Palombini, G, Galeotti, T (1986) Cytochrome P-450 deficiency and resistance to t-butyl hydroperoxide of hepatoma microsomal lipid peroxidation. Biochim. Biophys. Acta 876: pp. 220-225 CrossRef
  11. Zeytinoglu, H, Incesu, Z, Baser, KH (2003) Inhibition of DNA synthesis by carvacrol in mouse myoblast cells bearing a human N-RAS oncogene. Phytomedicine 10: pp. 292-299 CrossRef
  12. Kirimer, N, Baser, KHC, T眉men, G (1995) Carvacrol rich plants in Turkey. Chem. Nat. Compd. 31: pp. 37-41 CrossRef
  13. Liang, WZ, Chou, CT, Lu, T, Chi, CC, Tseng, LL, Pan, CC, Lin, KL, Kuo, CC, Jan, CR (2013) The mechanism of carvacrol-evoked [Ca2+]i rises and non-Ca2+-triggered cell death in OC2 human oral cancer cells. Toxicology 303: pp. 152-161 CrossRef
  14. Ultee, A, Smid, EJ (2001) Influence of carvacrol on growth and toxin production by Bacillus cereus. Int. J. Food Microbiol. 64: pp. 373-378 CrossRef
  15. Oussalah, M, Caillet, S, Saucier, L, Lacroix, M (2007) Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control 18: pp. 414-420 CrossRef
  16. Kim, YS, Lee, SJ, Hwang, JW, Kim, EK, Kim, SE, Kim, EH, Moon, SH, Jeon, BT, Park, PJ (2012) In vitro protective effects of Thymus quinquecostatus Celak extracts on t-BHP-induced cell damage through antioxidant activity. Food Chem. Toxicol. 50: pp. 4191-4198 CrossRef
  17. Hotta, M, Nakata, R, Katsukawa, M, Hori, K, Takahashi, S, Inoue, H (2010) Carvacrol, a component of thyme oil, activates PPAR伪 and 纬, and suppresses COX-2 expression. J. Lipid Res. 51: pp. 132-139 CrossRef
  18. Arunasree, KM (2010) Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231. Phytomedicine 17: pp. 581-588 CrossRef
  19. Guimar茫es, AG, Oliveira, GF, Melo, MS, Cavalcanti, SC, Antoniolli, AR, Bonjardim, LR, Silva, FA, Santos, JP, Rocha, RF, Moreira, JC, Ara煤jo, AA, Gelain, DP, Quintans-J煤nior, LJ (2010) Bioassay-guided evaluation of antioxidant and antinociceptive activities of carvacrol. Basic Clin. Pharmacol. 107: pp. 949-957 CrossRef
  20. Melo, FH, Moura, BA, Sousa, DP, Vasconcelos, SM, Macedo, DS, Fonteles, MM, Viana, GS, Sousa, FC (2011) Antidepressant-like effect of carvacrol (5-isopropyl-2-methylphenol) in mice: Involvement of dopaminergic system. Fund. Clin. Pharmacol. 25: pp. 362-367 CrossRef
  21. Melo, FH, Ven芒ncio, ET, Sousa, DP, Franca Fonteles, MM, Vasconcelos, SM, Viana, GS, Sousa, FC (2010) Anxiolytic-like effect of Carvacrol (5-isopropyl-2-methylphenol) in mice: Involvement with GABAergic transmission. Fund. Clin. Pharmacol. 24: pp. 437-443 CrossRef
  22. Aydin, Y, Kutlay, O, Ari, S, Duman, S, Uzuner, K, Aydin, S (2007) Hypotensive effects of carvacrol on the blood pressure of normotensive rats. Planta Med. 73: pp. 1365-1371 CrossRef
  23. Aristatile, B, Al-Numair, KS, Veeramani, C, Pugalendi, KV (2009) Antihyperlipidemic effect of carvacrol on d-galactosamine-induced hepatotoxic rats. J. Basic Clin. Physiol. Pharmacol. 20: pp. 15-27 CrossRef
  24. Kim, EK, Je, JY, Lee, SJ, Kim, YS, Hwang, JW, Sung, SH, Moon, SH, Jeon, BT, Kim, SK, Jeon, YJ, Park, PJ (2012) Chitooligosaccharides induce apoptosis in human myeloid leukemia HL-60 cells. Bioorg. Med. Chem. Lett. 22: pp. 6136-6138 CrossRef
  25. Zamzami, N, Maisse, C, Metivier, D, Kroemer, G (2007) Measurement of membrane permeability and permeability transition of mitochondria. Method. Cell Biol. 80: pp. 327-340 CrossRef
  26. Kamal, AA, Gomaa, A, Khafif, M, Hammad, AS (1989) Plasma lipid peroxides among workers exposed to silica or asbestos dusts. Environ. Res. 49: pp. 173-180 CrossRef
  27. Zhang, JH, Xu, M (2000) DNA fragmentation in apoptosis. Cell Res. 10: pp. 205-211 CrossRef
  28. Kung, G, Konstantinidis, K, Kitsis, RN (2011) Programmed necrosis, not apoptosis, in the heart. Circ. Res. 108: pp. 1017-1036 CrossRef
  29. Janero, DR (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Bio. Med. 9: pp. 515-540 CrossRef
  30. Wu, SJ, Ng, LT (2007) Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia linn, var abbreviata Ser.) in Taiwan. LWT-Food Sci. Technol. 41: pp. 323-330 CrossRef
  31. Rhee, SG, Chae, HZ, Kim, K (2005) Peroxiredoxins: A historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling. Free Radical. Bio. Med. 38: pp. 1543-1552 CrossRef
  32. Higuchi, Y (2004) Glutathione depletion-induced chromosomal DNA fragmentation associated with apoptosis and necrosis. J. Cell Mol. Med. 8: pp. 455-464 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  
• 出版者：The Korean Society of Food Science and Technology in co-publication with Springer
• ISSN：2092-6456

文摘

Carvacrol is a monoterpenic phenol present in Thymus quinquecostatus Celak. The protective effects of carvacrol against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage were investigated in human Chang cells. Cells treated with carvacrol extracts promoted Chang cell survival and protection was associated with stabilization of the mitochondrial membrane potential (MMP), prevention of oxidative stress-triggered reactive oxygen species (ROS), and lipid peroxidation (MDA production). In addition, Annexin V/PI, observed using Hoechst staining, indicated that carvacrol inhibited t-BHP-induced cell damage and stimulated the antioxidant capability of Chang cells due to elevation of glutathione (GSH) levels, which were reduced by t-BHP treatment. Carvacrol prevented oxidative stress-induced Chang cell damage via suppression of ROS and MDA levels, and increased GSH levels.