Molecular Identification and Characterization of a Gene Associated with the Onset of Tapping Panel Dryness (TPD) Syndrome in Rubber Tree (Hevea?brasiliensis Muell.) by mRNA Differential Display

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• 作者：Perumal Venkatachalam (1) (2)
  Arjunan Thulaseedharan (2)
  Kashchandra Raghothama (1)
  
• 关键词：mRNA differential display ; Translocase of the outer mitochondrial membrane ; Latex biosynthesis ; Cloning ; Healthy rubber tree ; High yielding clones
• 刊名：Molecular Biotechnology
• 出版年：2009
• 出版时间：January 2009
• 年：2009
• 卷：41
• 期：1
• 页码：42-52
• 全文大小：1869KB
• 参考文献：1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. / Nucleic Acids Research, / 25, 3389-402. doi:10.1093/nar/25.17.3389 . CrossRef
  2. Annamalainathan, K., Krishnakumar, R., & Jacob, J. (2001). Tapping induced changes in respiratory metabolism, ATP production and reactive oxygen species scavenging in / Hevea. / Journal of Rubber Research, / 4, 245-54.
  3. Anonymous. (2002). Mean yield during the fourth year of tapping. RRII Annual Report, pp. 23.
  4. Carginale, V., Maria, G., Capasso, C., Ionata, E., La Cara, F., Pastore, M., et al. (2004). Identification of genes expressed in response to phytoplasma infection in leaves of / Prunus?armeniaca by messenger RNA differential display. / Gene, / 332, 29-4. doi:10.1016/j.gene.2004.02.030 . CrossRef
  5. Chen, S., Peng, S., Huang, G., Wu, K., Fu, X., & Chen, Z. (2003). Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in / Hevea brasiliensis. / Plant Molecular Biology, / 51, 51-8. doi:10.1023/A:1020719420867 . CrossRef
  6. Chrestin, H. (1989). Biochemical aspects of bark dryness induced by overstimulation of rubber tree with Ethrel. In J. d’Auzac, L. Jacob & H. Chrestin (Eds.), / Physiology of rubber tree latex (pp. 341-41). Boca Raton, FL: CRC Press.
  7. Chrestin, H., Gidrol, X., & Kush, A. (1997). Towards a latex molecular diagnostic of yield potential and the genetic engineering of the rubber tree. / Euphytica, / 96, 77-2. doi:10.1023/A:1002950300536 . CrossRef
  8. Coupé, M., & Chrestin, H. (1989). Physico-chemical and biochemical mechanisms of hormonal (ethylene) stimulation. In J. d’Auzac, L. Jacob & H. J. Chrestin (Eds.), / Physiology of the rubber tree latex (pp. 295-19). Boca Raton, FL: CRC Press.
  9. d’Auzac, J., & Jacob, J. L. (1989). The composition of latex from / Hevea brasiliensis as a laticiferous cytoplasm. In J. d’Auzac, L. Jacob & H. Chrestin (Eds.), / Physiology of the rubber tree latex (pp. 59-6). Boca Raton, FL: CRC Press.
  10. de Fa?, E., Ebant, Ch., & Jacob, J. L. (1989). Cytology and cytochemistry of the laticiferous system. In J. d’Auzac, L. Jacob & H. Chrestin (Eds.), / Physiology of the rubber tree latex (pp. 15-9). Boca Raton, FL: CRC Press.
  11. de Fa?, E., & Jacob, J. L. (1989). Anatomical organization of the laticiferous system in the bark of / Hevea brasiliensis. In J. d’Auzac, L. Jacob & H. Chrestin (Eds.), / Physiology of the rubber tree latex (pp. 3-4). Boca Raton, FL: CRC Press.
  12. Dian, K., Sangare, A., & Diopoh, J. K. (1995). Evidence for specific variation of protein pattern during tapping panel dryness condition development in / Hevea brasiliensis. / Plant Science, / 105, 207-16. CrossRef
  13. Fan, X. W., & Yang, S. Q. (1994). Cause of disease and hypothesis on tapping panel dryness of / Hevea brasiliensis. / Chinese Journal of Tropical Crops Research, / 18, 43-8.
  14. Goulao, L. F., & Oliveira, C. M. (2007). Molecular identification of novel differentially expressed mRNAs up-regulated during ripening of apples. / Plant Science, / 172, 306-18. CrossRef
  15. Han, K. H., Shin, D. H., Yang, J., Kim, I. J., Oh, S. K., & Chow, K. S. (2000). Genes expressed in the latex of / Hevea brasiliensis. / Tree Physiology, / 20, 503-10.
  16. Heins, L., & Schmitz, U. K. (1996). A receptor for protein import into potato mitochondria. / Plant Journal, / 9, 829-39. CrossRef
  17. Krishnakumar, R., Annamalainathan, K., & Jacob, J. (2001). Tapping panel dryness syndrome in / Hevea increases dark respiration but not ATP status. / Indian Journal of Natural Rubber Research, / 14, 14-9.
  18. Krishnakumar, R., Rittu, M., Sreelatha, S., & Jacob, J. (2006). Endogenous ethylene and oxidative stress in / Hevea brasiliensis. In J. Jacob, R. Krishnakumar & M. M. Mathew (Eds.), / Tapping panel dryness of rubber trees (pp. 116-24). Kottayam: Rubber Research Institute of India.
  19. Krishnakumar, R., Sreelatha, S., Thomas, M., Gopalakrishnan, J., Jacob, J., & Sethuraj, M. R. (1999). Biochemical composition of soft bark tissues in / Hevea affected by tapping panel dryness. / Indian Journal of Natural Rubber Research, / 12, 92-9.
  20. Kyte, J., & Doolittle, R. (1982). A simple method for displaying the hydropathic character of a protein. / Journal of Molecular Biology, / 157, 105-32. CrossRef
  21. Lang, P., Zhang, C., Ebel, R. C., Dane, F., & Dozier, W. A. (2005). Identification of cold acclimated genes in leaves of / Citrus unshiu by mRNA differential display. / Gene, / 359, 111-18. CrossRef
  22. Liang, P., & Pardee, A. B. (1992). Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. / Science, / 257, 2541-543. CrossRef
  23. Lister, R., Chew, O., Lee, M. N., Heazlewood, J. L., Clifton, R., Parker, K. L., et al. (2004). Transcriptomic and proteomic characterization of the / Arabidopsis mitochondrial protein import apparatus and its response to mitochondrial mysfunction. / Plant Physiology, / 134, 777-89. CrossRef
  24. Pellegrin, F., Duran-Vila, N., Van Munster, M., & Nandris, D. (2007). Rubber tree ( / Hevea brasiliensis) trunk phloem necrosis: Aetiological investigations failed to confirm any biotic causal agent. / Forest Pathology, / 37, 9-1. CrossRef
  25. Sookmark, U., Kongsawadworakul, P., Narangajavana, J., & Chrestin, H. (2006). Studies on oxidative stress in rubber tree latex and its relation to panel dryness. In J. Jacob, R. Krishnakumar & M. M. Mathew (Eds.), / Tapping panel dryness of rubber trees (pp. 106-15). Kottayam: Rubber Research Institute of India.
  26. Sookmark, U., Pujade-Renaud, V., Chrestin, H., Lacotem, R., Naiyanetr, C., Seguin, M., et al. (2002). Characterization of polypeptides accumulated in the latex cytosol of rubber trees affected by the tapping panel dryness syndrome. / Plant and Cell Physiology, / 43, 1323-333. CrossRef
  27. Sweetlove, L. J., Heazlewood, J. L., Herald, V., Holtzapffel, R., Day, D. A., Leaver, C. J., et al. (2002). The impact of oxidative stress on / Arabidopsis mitochondria. / Plant Journal, / 32, 891-04. CrossRef
  28. Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. / Nucleic Acids Research, / 22, 4673-680. CrossRef
  29. Torres, G. A., Pflieger, S., Corre-Menguy, F., Mazubert, C., Hartmann, C., & Lelandais-Brière, C. (2006). Identification of novel drought-related mRNAs in common bean roots by differential display RT-PCR. / Plant Science, / 171, 300-07. CrossRef
  30. Venkatachalam, P., Thanseem, I., & Thulaseedharan, A. (1999). A rapid and efficient method for isolation of RNA from bark tissues of / Hevea brasiliensis. / Current Science, / 77, 101-03.
  31. Venkatachalam, P., Thulaseedharan, A., & Raghothama, K. G. (2007). Identification of expression profiles of tapping panel dryness (TPD) associated genes from the latex of rubber tree ( / Hevea brasiliensis Muell. Arg.). / Planta, / 226, 499-15. CrossRef
  32. Werhahn, W., Jansch, L., & Braun, H.-P. (2003). Identification of novel subunits of the TOM complex from / Arabidopsis thaliana. / Plant Physiology & Biochemistry, / 41, 407-16. CrossRef
  33. Werhahn, W., Niemeyer, A., Jansch, L., Kruft, V., Schmitz, U. K., & Braun, H.-P. (2001). Purification and characterisation of the preprotein translocase of the outer mitochondrial membrane from / Arabidopsis: identification of multiple forms of Tom20. / Plant Physiology, / 125, 943-54. CrossRef
  34. Zamzami, N., Hirsch, T., Dallaporta, B., Petit, P. X., & Kroemer, G. (1997). Mitochondrial implication in accidental and programmed cell death: apoptosis and necrosis. / Journal of Bioenergetics and Biomembrane, / 29, 185-93. CrossRef
  35. Zhu, L. T., & Altmann, S. W. (2005). mRNA and 18S RNA coapplication-reverse transcription for quantitative gene expression analysis. / Analytical Biochemistry, / 345, 102-09. CrossRef
  
• 作者单位：Perumal Venkatachalam (1) (2)
  Arjunan Thulaseedharan (2)
  Kashchandra Raghothama (1)
  
  1. Plant Molecular Biology Lab, Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907, USA
  2. Biotechnology Division, Rubber Research Institute of India, Kottayam, 86 009, Kerala, India
  

文摘

In rubber tree (Hevea?brasiliensis), tapping panel dryness (TPD) syndrome is considered as a complex physiological disorder which affects latex biosynthesis. To identify differentially expressed genes between healthy and TPD-affected trees, mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) analysis was performed. We isolated 10 differentially expressed cDNA fragments of which one cDNA encoding a putative TOM20 like protein was identified. The cDNA (1,024?bp), corresponding to the HbTOM20 gene (H evea b rasiliensis Translocase of the Outer Mitochondrial Membrane), contained an open reading frame to code for 202 amino acid protein with a theoretical pI value of 9.5 and the calculated protein M W was 23.5?kDa. The predicted amino acid sequence contained conserved domains of TOM20 like proteins in the N-terminal. The protein HbTOM20 has 32% and 27% similarity to Populus TOM20 and Solanum TOM20, respectively. Both semi-quantitative RT-PCR and Northern blot results revealed that the HbTOM20 expression was significantly down-regulated in TPD-affected trees compared to healthy one. Accumulation of HbTOM20 mRNA transcripts was significantly higher in the bark tissues collected from healthy region than that of partially affected by TPD (partially dried) while barely detectable in completely TPD-affected area. Differential expression pattern was noticed in three rubber clones representing various degrees of TPD tolerance. These results suggest that down-regulation of HbTOM20 in TPD-affected trees may play an important role in alteration of mitochondrial metabolism resulting in impaired latex biosynthesis.