Novel drought-responsive regulatory coding and non-coding transcripts from Oryza Sativa L.
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- 作者：Seon-Ju Shin ; Hongryul Ahn ; Inuk Jung ; Sungmin Rhee ; Sun Kim…
- 关键词：Rice ; Drought ; Coding and noncoding transcripts ; NGS ; Bioinformatics
- 刊名：Genes & Genomics
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：38
- 期：10
- 页码：949-960
- 全文大小：1,309 KB
- 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
- 出版者：Springer Netherlands
- ISSN：2092-9293
- 卷排序：38
文摘
Drought-stress can cause major economic loss and is a serious issue to address in agriculture. Defining the molecular pathways in how a plant responds to drought-stress may prove valuable in developing new drought-resistant plants. In this study, we identified several novel drought-responsive regulatory coding and noncoding transcripts in rice, Oryza sativa L., using the next generation sequencing (NGS) technique and bioinformatics analyses. We produced comprehensive NGS RNA sequencing data for mRNA, small RNA, and long noncoding RNA at the experimental conditions of aeration without watering for 1 and 6 h in compared with the 0 h control. We performed bioinformatics analysis to identify novel drought-responsive transcription factors (TFs), novel drought-responsive micro RNA (miRNA), and novel drought-responsive long noncoding RNAs (lncRNAs). These transcripts were validated by quantitative real-time PCR (qRT-PCR). We identified 18 TFs, ten lncRNAs and one miRNA as being novel drought-responsive regulatory transcripts. Computational analysis using a gene regulatory network showed that these transcripts were related to regulation of response to drought-stress.