The Tendon Injury Response is Influenced by Decorin and Biglycan
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- 作者：Andrew A. Dunkman (1)
Mark R. Buckley (1)
Michael J. Mienaltowski (2)
Sheila M. Adams (2)
Stephen J. Thomas (1)
Lauren Satchell (1)
Akash Kumar (1)
Lydia Pathmanathan (1)
David P. Beason (1)
Renato V. Iozzo (3)
David E. Birk (2)
Louis J. Soslowsky (1) - 关键词：Tendon ; Injury ; Biglycan ; Decorin ; Proteoglycan ; Extracellular matrix ; SLRP ; Healing
- 刊名：Annals of Biomedical Engineering
- 出版年：2014
- 出版时间：March 2014
- 年：2014
- 卷：42
- 期：3
- 页码：619-630
- 全文大小：1,687 KB
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Mark R. Buckley (1)
Michael J. Mienaltowski (2)
Sheila M. Adams (2)
Stephen J. Thomas (1)
Lauren Satchell (1)
Akash Kumar (1)
Lydia Pathmanathan (1)
David P. Beason (1)
Renato V. Iozzo (3)
David E. Birk (2)
Louis J. Soslowsky (1)
1. The McKay Orthopaedic Research Laboratory, University of Pennsylvania, 424 Stemmler Hall, 3450 Hamilton Walk, Philadelphia, PA, 19104, USA
2. Department of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs Blvd, MDC 8, Tampa, FL, 33612, USA
3. Department of Pathology, Anatomy & Cell Biology, Thomas Jefferson University, 1020 Locust Street, Jefferson Alumni Hall, Suite 249, Philadelphia, PA, 19107, USA - ISSN：1573-9686
文摘
Defining the constituent regulatory molecules in tendon is critical to understanding the process of tendon repair and instructive to the development of novel treatment modalities. The purpose of this study is to define the structural, expressional, and mechanical changes in the tendon injury response, and elucidate the roles of two class I small leucine-rich proteoglycans (SLRPs). We utilized biglycan-null, decorin-null and wild type mice with an established patellar tendon injury model. Mechanical testing demonstrated functional changes associated with injury and the incomplete recapitulation of mechanical properties after 6?weeks. In addition, SLRP deficiency influenced the mechanical properties with a marked lack of improvement between 3 and 6?weeks in decorin-null tendons. Morphological analyses of the injury response and role of SLRPs demonstrated alterations in cell density and shape as well as collagen alignment and fibril structure resulting from injury. SLRP gene expression was studied using RT-qPCR with alterations in expression associated with the injured tendons. Our results show that in the absence of biglycan initial healing may be impaired while in the absence of decorin later healing is clearly diminished. This suggests that biglycan and decorin may have sequential roles in the tendon response to injury.