Enhanced extracellular production of recombinant proteins in Escherichia coli by co-expression with Bacillus cereus phospholipase C
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- 作者：Lingqia Su ; Qi Jiang ; Lingang Yu ; Jing Wu
- 关键词：Bacillus cereus phospholipase C ; Co ; expression ; Extracellular protein production ; Membrane permeability ; Foam formation
- 刊名：Microbial Cell Factories
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：16
- 期：1
- 全文大小：1770KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Applied Microbiology; Biotechnology; Microbiology; Microbial Genetics and Genomics; Enzymology; Genetic Engineering;
- 出版者：BioMed Central
- ISSN：1475-2859
- 卷排序：16
文摘
BackgroundOur laboratory has reported a strategy for improving the extracellular production of recombinant proteins through co-expression with Thermobifida fusca cutinase, which increases membrane permeability via its phospholipid hydrolysis activity. However, the foam generated by the lysophospholipid product makes the fermentation process difficult to control in a fermentor. Phospholipase C (PLC) catalyzes the hydrolysis of phospholipids to produce sn1,2-diacylglycerides and organic phosphate, which do not induce foam formation. Therefore, co-expression with Bacillus cereus PLC was investigated as a method to improve the extracellular production of recombinant proteins.