SERS Study of Histamine by Using Silver Film over Nanosphere Structure
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- 作者：Wen-Chi Lin (1) (2)
Tsong-Ru Tsai (1)
Hsiang-Lin Huang (1)
Chyuan Yuan Shiau (3)
Hai-Pang Chiang (1) (4) (5) - 关键词：Surface ; enhanced Raman scattering (SERS) ; Nanosphere lithography (NSL) ; Silver film over nanosphere (AgFON) ; Histamine
- 刊名：Plasmonics
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：7
- 期：4
- 页码：709-716
- 全文大小：445KB
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Tsong-Ru Tsai (1)
Hsiang-Lin Huang (1)
Chyuan Yuan Shiau (3)
Hai-Pang Chiang (1) (4) (5)
1. Institute of Optoelectronic Sciences, National Taiwan Ocean University, No. 2 Pei-Ning Rd., 202, Keelung, Taiwan
2. Holistic Medical Device Development Center, Chung Yuan Christian University, Chung-Li, Taiwan
3. Department of Food Sciences, National Taiwan Ocean University, Keelung, Taiwan
4. Institute of Physics, Academia Sinica, Taipei, Taiwan
5. Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu, Taiwan - ISSN：1557-1963
文摘
Optical properties of histamine and l-histidine have been analyzed by using surface-enhanced Raman scattering (SERS). A silver film over nanosphere (AgFON) structure with 120-nm-thick silver film on polystyrene nanospheres 1,000?nm in diameter is fabricated by nanosphere lithography to enhance the Raman signal excited at the laser wavelength of 532?nm. Normal Raman spectrum and the SERS spectrum of histamine and l-histidine were compared. Further, vibration modes of these molecules were calculated by using density functional method. In the SERS experiment, we were able to measure the Raman spectrum with a histamine concentration as less as 100?pM. This sensitivity is higher than that from high-performance liquid chromatography.