Factorial design and response surface optimization of crude violacein for Chromobacterium violaceum production

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• 作者：Armando S. Mendes ; Jo?o E. de Carvalho ; Marta C. T. Duarte ; Nelson Dur¨¢n and Roy E. Bruns
• 关键词：Chromobacterium violaceum ; experimental design ; fermentation broth ; medium optimization ; violacein
• 刊名：Biotechnology Letters
• 出版年：2001
• 出版时间：2001
• 年：2001
• 卷：23
• 期：23
• 页码：1963-1969
• 全文大小：118 KB

文摘

Initially an eleven variable, sixteen assay 215–11 fractional factorial design, was used to determine the key factors in the production of violacein produced by Chromobacterium violaceum, CCT 3496. Subsequently five and three factor central composite designs were executed to determine response surfaces with the aim of optimizing cellular mass and crude violacein production. The 7.5 g l–1 dry cell mass and 0.17 g l–1 crude violacein productions obtained with our initial culture medium were increased to 21 g l–1 and 0.43 g l–1, respectively, for a medium investigated in the three factor design.