PEG-mediated transient gene expression and silencing system in maize mesophyll protoplasts: a valuable tool for signal transduction study in maize
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- 作者：Jianmei Cao (1) (2)
Dongmei Yao (1) (2)
Fan Lin (1)
Mingyi Jiang (1) (2) - 关键词：PEG ; mediated ; Transfection ; Protoplast transient expression ; RNAi ; BiFC ; Maize
- 刊名：Acta Physiologiae Plantarum
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：36
- 期：5
- 页码：1271-1281
- 全文大小：1,860 KB
- 参考文献：1. Abel S, Theologis A (1994) Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene-expression. Plant J 5:421-27 CrossRef
2. An CI, Sawada A, Fukusaki E, Kobayashi A (2003) A transient RNA interference assay system using Arabidopsis protoplasts. Biosci Biotechnol Biochem 67:2674-677 CrossRef
3. An CI, Sawada A, Kawaguchi Y, Fukusaki E, Kobayashi A (2005) Transient RNAi induction against endogenous genes in Arabidopsis protoplasts using in vitro-prepared double-stranded RNA. Biosci Biotechnol Biochem 69:415-18 CrossRef
4. Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL et al (2002) MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415:977-83 CrossRef
5. Bart R, Chern M, Park CJ, Bartley L, Ronald PC (2006) A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts. Plant Methods 2:13 CrossRef
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-54 CrossRef
7. Chen SX, Halkier BA (2000) Characterization of glucosinolate uptake by leaf protoplasts of / Brassica napus. J Biol Chem 275:22955-2960 CrossRef
8. Cho JI, Ryoo N, Eom JS, Lee DW, Kim HB et al (2009) Role of the rice hexokinases OsHXK5 and OsHXK6 as glucose sensors. Plant Physiol 149:745-59 CrossRef
9. De Sutter V, Vanderhaeghen R, Tilleman S, Lammertyn F, Vanhoutte I et al (2005) Exploration of jasmonate signalling via automated and standardized transient expression assays in tobacco cells. Plant J 44:1065-076 CrossRef
10. Ding Y, Cao J, Ni L, Zhu Y, Zhang A, Tan M, Jiang M (2013) ZmMPK5 is involved in abscisic acid (ABA)-induced antioxidant defence and functions upstream of ZmMPK5 in abscisic acid signaling in maize. J Exp Bot 64:871-84 CrossRef
11. Gao X, Wheeler T, Li Z, Kenerley CM, He P et al (2011) Silencing GhNDR1 and GhMKK2 compromises cotton resistance to verticillium wilt. Plant J 66:293-05 CrossRef
12. Hachez C, Moshelion M, Zelazny E, Cavez D, Chaumont F (2006) Localization and quantification of plasma membrane aquaporin expression in maize primary root: a clue to understanding their role as cellular plumbers. Plant Mol Biol 62:305-23 CrossRef
13. Hillmer S, Gilroy S, Jones RL (1993) Visualizing enzyme secretion from individual barley ( / Hordeum vulgare) aleurone protoplasts. Plant Physiol 102:279-86 CrossRef
14. Hoang MHT, Nguyen XC, Lee K, Kwon YS, Pham HTT et al (2012) Phosphorylation by AtMPK6 is required for the biological function of AtMYB41 in Arabidopsis. Biochem Biophy Res Commun 422:181-86 CrossRef
15. Hwang I, Sheen J (2001) Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 413:383-89 CrossRef
16. Jefferson RA (1987) Assaying chimeric gene in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387-05 CrossRef
17. Jiang M, Zhang J (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265-273 CrossRef
18. Jones H, Ooms G, Jones MGK (1989) Transient gene-expression in electroporated solanum protoplasts. Plant Mol Biol 13:503-11 CrossRef
19. Kim HY, Cote GG, Crain RC (1993) Potassium channels in samanea-saman protoplasts controlled by phytochrome and the biological clock. Science 260:960-62 CrossRef
20. Lin F, Ding H, Wang J, Zhang H, Zhang A et al (2009) Positive feedback regulation of maize NADPH oxidase by mitogen-activated protein kinase cascade in abscisic acid signalling. J Exp Bot 60:3221-238 CrossRef
21. Liu XM, Nguyen XC, Kim KE, Han HJ, Yoo J et al (2013) Phosphorylation of the zinc finger transcriptional regulator ZAT6 by MPK6 regulates Arabidopsis seed germination under salt and osmotic stress. Biochem Biophy Res Commun 430:1054-059 CrossRef
22. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490-98 CrossRef
23. Nguyen XC, Kim SH, Lee K, Kim KE, Liu XM et al (2012) Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase. Plant Cell Rep 31:737-45 CrossRef
24. Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD (1985) Direct gene-transfer to cells of a graminaceous monocot. Mol Gen Genet 199:183-88 CrossRef
25. Schweizer P, Pokorny J, Schulze-Lefert P, Dudler R (2000) Double-stranded RNA interferes with gene function at the single-cell level in cereals. Plant J 24:895-03 CrossRef
26. Sheen J (1993) Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J 12:3497-505
27. Sheen J (1996) Ca2+ dependent protein kinases and stress signal transduction in plants. Science 274:1900-902 CrossRef
28. Sheen J (2001) Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol 127:1466-475 CrossRef
29. Su CF, Wang YC, Hsieh TH, Lu CA, Tseng TH et al (2010) A novel MYBS3-dependent pathway confers cold tolerance in rice. Plant Physiol 153:145-58 CrossRef
30. Teige M, Scheikl E, Eulgem T, Doczi R, Lchimura K et al (2004) The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis. Mol Cell 15:141-52 CrossRef
31. Terashita K, Kato S, Sata M, Inoue S, Nakamura H et al (2006) Increased endothelin-1 levels of BAL fluid in patients with pulmonary sarcoidosis. Respirology 11:145-51 CrossRef
32. Timmons L, Fire A (1998) Specific interference by ingested dsRNA. Nature 395:854 CrossRef
33. Waadt R, Schmidt LK, Lohse M, Hashimoto K, Bock R, Kudla J (2008) Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J 56:505-16 CrossRef
34. Xu J, Li H-D, Chen L-Q, Wang Y, Liu L-L et al (2006) A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ transporter AKT1 in Arabidopsis. Cell 125:1347-360 CrossRef
35. Yanagisawa S, Sheen J (1998) Involvement of maize Dof zinc finger proteins in tissue-specific and light-regulated gene expression. Plant Cell 10:75-9
36. Yoo SD, Cho YH, Sheen J (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2:1565-572 CrossRef
37. Zhai ZY, Sooksa-nguan T, Vatamaniuk OK (2009) Establishing RNA interference as a reverse-genetic approach for gene functional analysis in protoplasts. Plant Physiol 149:642-52 CrossRef
38. Zhang A, Jiang M, Zhang J, Tan M, Hu X (2006) Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. Plant Physiol 141:475-87 CrossRef
39. Zhang H, Ni L, Liu Y, Wang Y, Zhang A et al (2012) The C2H2-type zinc finger protein ZFP182 is involved in abscisic acid-induced antioxidant defense in rice. J Integr Plant Biol 54:500-10 CrossRef
40. Zhu Y, Zuo M, Liang Y, Jiang M, Zhang J et al (2013) MAP65-1a positively regulates H2O2 amplification and enhances brassinosteroid-induced antioxidant defence in maize. J Exp Bot 64:3787-802 CrossRef - 作者单位：Jianmei Cao (1) (2)
Dongmei Yao (1) (2)
Fan Lin (1)
Mingyi Jiang (1) (2)
1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China
2. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China - ISSN：1861-1664
文摘
Polyethylene glycol (PEG)-mediated transient gene expression and silencing in protoplasts is widely applied in model plants such as Arabidopsis thaliana and rice. Here, we developed an efficient transient gene expression system based on the PEG-mediated method both in etiolated and green maize mesophyll protoplasts. The results showed that both yellow fluorescent protein encoding gene and glucuronidase encoding gene were efficiently expressed in maize protoplasts. More importantly, double-stranded RNAs (dsRNAs) can also be transfected into maize protoplasts by the PEG-mediated method to specifically silence exogenous and endogenous genes. Our results showed that dsRNA can be used to knockdown both exogenous and endogenous gene expression. Furthermore, bimolecular fluorescence complementation system for the detection of protein–protein interactions in maize protoplasts was developed. We also overexpressed and knockdowned the mitogen-activated protein kinase encoding gene ZmMPK5 to investigate the role of ZmMPK5 in abscisic acid (ABA)-induced antioxidant defense in maize protoplasts. This method here we reported will be valuable for signal transduction study in maize.