Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco

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• 作者：Chen Lijuan ; Guo Huiming ; Lin Yi ; Cheng Hongmei
• 关键词：Chalcone synthase ; Eupatorium adenophorum ; Gene expression ; Salt stress
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：34
• 期：5
• 页码：885-894
• 全文大小：2,633 KB
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• 作者单位：Chen Lijuan (1) (2)
  Guo Huiming (1)
  Lin Yi (2)
  Cheng Hongmei (1)
  
  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
  2. School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message EaCHS1 functions in the tolerance of plantlets to salinity stress by maintaining ROS homeostasis. Abstract Chalcone synthase (CHS) is an essential enzyme in the biosynthesis of flavonoids. Expression of CHS is governed by a wide range of environmental stimuli, including UV light, pathogen attack, and circadian clocks. However, little research exists on the relationship between CHS and salinity stress. In this work, we constructed separate overexpression and RNA interference vectors of EaCHS1, and transferred them into tobacco. Overexpression of EaCHS1 increased the production of downstream flavonoids and the expressions of related genes in the phenylpropanoid pathway. It also improved resistance to salinity stress during seed germination and root development. In contrast, heterologous silencing of endogenous CHS in tobacco by a conserved EaCHS1 fragment had opposite effect. Together, our results indicated that changing the expression level of EaCHS1 in plants alters the accumulation of flavonoids and regulates plantlet tolerance to salinity stress by maintaining ROS homeostasis.