MicroRNA expression profile in exosome discriminates extremely severe infections from mild infections for hand, foot and mouth disease

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• 作者：Hong-Ling Jia (10)
  Chun-Hui He (9)
  Zhuo-Ya Wang (12)
  Yu-Fen Xu (9)
  Gen-Quan Yin (9)
  Li-Jia Mao (9)
  Chao-Wu Liu (11)
  Li Deng (9)
  
  10. Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes ; Institute of Life and Health Engineering ; College of Life Science and Technology ; Jinan University ; Guangzhou ; 510632 ; Guangdong ; China
  9. Guangzhou Women and Children鈥檚 Medical Center ; Guangzhou ; 510120 ; Guangdong ; China
  12. Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances ; School of Basic Courses ; Guangdong Pharmaceutical University ; Guangzhou ; 510006 ; Guangdong ; China
  11. Guangdong Institute of Microbiology/State Key Laboratory of Applied Microbiology Southern China/Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application ; Guangzhou ; 510070 ; Guangdong ; China
  
• 关键词：Exosomal microRNA Profile ; HFMD ; Diagnosis ; Biomarker
• 刊名：BMC Infectious Diseases
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：1,163 KB
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  40. The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-2334/14/506/prepub
  
• 刊物主题：Infectious Diseases; Parasitology; Medical Microbiology; Tropical Medicine; Internal Medicine;
• 出版者：BioMed Central
• ISSN：1471-2334

文摘

Background Changes of miRNAs in exosome have been reported in different disease diagnosis and provided as potential biomarkers. In this study, we compared microRNA profile in exosomes in 5 MHFMD and 5 ESHFMD as well as in 5 healthy children. Methods Different expression of miRNAs in exosomes across all the three groups were screened using miRNA microarray method. Further validated test was conducted through quantitative real-time PCR assays with 54 exosome samples (18 ESHFMD, 18 MHFMD, and 18 healthy control). The judgment accuracy was then estimated by the receiver operating characteristic (ROC) curve analysis; and the specificity and sensitivity were evaluated by the multiple logistic regression analysis. Results There were 11 different miRNAs in exosomes of MHFMD and ESHFMD compared to healthy children, of which 4 were up-regulated and 7 were down-regulated. Further validation indicated that the 4 significant differentially expressed candidate miRNAs (miR-671-5p, miR-16-5p, miR-150-3p, and miR-4281) in exosome showed the same changes as in the microarray analysis, and the expression level of three miRNAs (miR-671-5p, miR-16-5p, and miR-150-3p) were significantly different between MHFMD or ESHFMD and the healthy controls. The accuracy of the test results were high with the under curve (AUC) value range from 0.79 to 1.00. They also provided a specificity of 72%-100% and a sensitivity of 78%-100%, which possessed ability to discriminate ESHFMD from MHFMD with the AUC value of 0.76-0.82. Conclusions This study indicated that the exosomal miRNA from patients with different condition of HFMD express unique miRNA profiles. Exosomal miRNA expression profiles may provide supplemental biomarkers for diagnosing and subtyping HFMD infections.