Development, characterization and cross-species amplification of new microsatellite primers from an endemic species Cinnamomum kanehirae (Lauraceae) in Taiwan
详细信息 查看全文
- 作者：Kuo-Hsiang Hung (1)
Chia-Hung Lin (2)
Huei-Chuan Shih (3)
Yu-Chung Chiang (4)
Li-Ping Ju (5) - 关键词：Cinnamomum ; Conservation ; Endemic species ; Microsatellite
- 刊名：Conservation Genetics Resources
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：6
- 期：4
- 页码：911-913
- 全文大小：160 KB
- 参考文献：1. Chiang YC, Shih HC, Huang MC, Ju LP, Hung KH (2012) The characterization of microsatellite loci from an endemic tree / Litsea hypophaea (Lauraceae) in Taiwan. Am J Bot 99:e251鈥揺254 CrossRef
2. Excoffier L, Laval G, Schneider S (2007) ARLEQUIN (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47鈥?0
3. Kalendar R, Lee D, Schulman AH (2011) Java web tools for PCR,聽in silico聽PCR, and oligonucleotide assembly and analysis. Genomics 98:137鈥?44 CrossRef
4. Rousset F (2008) GENEPOP鈥?07: a complete reimplementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103鈥?06 CrossRef
5. Shen YC, Wang YH, Chou YC, Chen CF, Lin LC, Chang TT, Tien JH, Chou CJ (2004) Evaluation of the anti-inflammatory activity of zhankuic acids isolated from the fruiting bodies of / Antrodia camphorata. Planta Med 70:310鈥?14 CrossRef - 作者单位：Kuo-Hsiang Hung (1)
Chia-Hung Lin (2)
Huei-Chuan Shih (3)
Yu-Chung Chiang (4)
Li-Ping Ju (5)
1. Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, 912, Taiwan
2. Department of Forestry, Pingtung University of Science and Technology, Pingtung, 912, Taiwan
3. Department of Nursing, Meiho University, Pingtung, 912, Taiwan
4. Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan
5. Botanical Garden Division, Taiwan Forestry Research Institute, Taipei, 100, Taiwan - ISSN：1877-7260
文摘
Cinnamomum kanehirae, an endemic species in Taiwan, has become rare and endangered because of intensive logging and illegal felling performed to obtain medicinal fungus. To facilitate C. kanehirae conservation, 15 novel microsatellites were developed using a magnetic bead enrichment method. Most of these markers were obtained using cross-species amplification. The number of alleles and observed and expected heterozygosities across loci ranged from 2 to 13, 0.000 to 1.000, and 0.152 to 0.897. These novel markers are useful for population and conservation genetic studies on C. kanehirae and for developing appropriate conservation strategies.