Astaxanthin Interacts with Selenite and Attenuates Selenite-Induced Cataractogenesis
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- 作者：Jiahn-Haur Liao† ; ; Chien-Sheng Chen† ; ; Timothy J. Maher‡ ; ; Chiung-Yueh Liu§ ; ; Mei-Hsiang Lin§ ; ; Tzu-Hua Wu*§ ;
- 刊名：Chemical Research in Toxicology
- 出版年：2009
- 出版时间：March 16, 2009
- 年：2009
- 卷：22
- 期：3
- 页码：518-525
- 全文大小：211K
- 年卷期：v.22,no.3(March 16, 2009)
- ISSN：1520-5010
文摘
Selenite, the most commonly encountered toxic form of selenium, in overdose, is used to induce cataracts in rats. This study demonstrated that selenite, but not selenate, would interact with the carotenoid astaxanthin (ASTX), as determined using isothermal titration calorimetry and NMR. The maximum absorption of ASTX decreased with increasing selenite concentration, indicating that the conjugated system of ASTX was changed by selenite. Such interactions between ASTX and selenite were also supported by the attenuation of selenite-induced turbidity by ASTX (0−12.5 μM) in vitro. In vivo experiments also showed that ASTX attenuated selenite-induced cataractogenesis in rats. In summary, this is the first report of a direct interaction of ASTX with selenite. This interaction is supported by an in vitro assay and may be partially responsible for the ASTX observed in vivo protection against selenite-induced cataractogenesis.