An Unbiased Approach To Identify Endogenous Substrates of 鈥淗istone鈥?Deacetylase 8
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- 作者：David E. Olson ; Namrata D. Udeshi ; Noah A. Wolfson ; Carol Ann Pitcairn ; Eric D. Sullivan ; Jacob D. Jaffe ; Tanya Svinkina ; Ted Natoli ; Xiaodong Lu ; Joshiawa Paulk ; Patrick McCarren ; Florence F. Wagner ; Doug Barker ; Eleanor Howe ; Fanny Lazzaro ; Jennifer P. Gale ; Yan-Ling Zhang ; Aravind Subramanian ; Carol A. Fierke ; Steven A. Carr ; Edward B. Holson
- 刊名：ACS Chemical Biology
- 出版年：2014
- 出版时间：October 17, 2014
- 年：2014
- 卷：9
- 期：10
- 页码：2210-2216
- 全文大小：449K
- ISSN：1554-8937
文摘
Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction with sophisticated proteomics methods to identify novel non-histone nuclear substrates of HDAC8, including the tumor suppressor ARID1A. These newly discovered substrates of HDAC8 are involved in diverse biological processes including mitosis, transcription, chromatin remodeling, and RNA splicing and may help guide therapeutic strategies that target the function of HDAC8.