Characterization of Thermolide Biosynthetic Genes and a New Thermolide from Sister Thermophilic Fungi

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• 作者：Xuemei Niu ; Li Chen ; Qun Yue ; Baile Wang ; Junxian Zhang ; Chunyan Zhu ; Keqin Zhang ; Gerald F. Bills ; Zhiqiang An
• 刊名：Organic Letters
• 出版年：2014
• 出版时间：July 18, 2014
• 年：2014
• 卷：16
• 期：14
• 页码：3744-3747
• 全文大小：254K
• ISSN：1523-7052

文摘

Prior chemical analysis of obligate thermophilic fungus Talaromyces thermophilus led to the discovery of thermolides A鈥揊, six previously undescribed members of the lactam-bearing macrolactone class. A combination of chemical screening, genome analyses, and genetic manipulation led to the identification of the thermolide biosynthetic genes from sister thermophilic fungi T. thermophilus and Thermomyces lanuginosus and a new thermolide. The biosynthetic locus for the thermolides鈥?mixed polyketide/amino acid structure encodes a hybrid polyketide synthase鈥搉onribosomal peptide synthetase (PKS鈥揘RPS). Our results reveal the first fungal hybrid iterative PKS鈥揘RPS genes involved in the biosynthesis of bacterial-like hybrid macrolactones instead of typical fungal tetramic acids-containing metabolites. The finding provides an insight into the convergent biosynthetic end products that bridge the gap between the modular and iterative PKS鈥揘RPS hybrids.